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R A Ludwig

Publications and source records attributed to R A Ludwig.

33 records · Page 2Linked to original sources
PubMed1984

Rhizobium sp. strain ORS571 ammonium assimilation and nitrogen fixation.

Among rhizobia studied, Rhizobium sp. strain ORS571 alone grew unambiguously on N2 as sole N source. In ORS571 , only the glutamine synthetase (GS)-glutamate synthase ( GOGAT ) pathway assimilated ammonium. However, ORS571 exhibited two unique physiological aspects of this pathway: ORS571 had only GS I, whereas all other Rhizobiaceae studied had both GS I and GS II, and both NADPH- and NADH-dependent GOGAT activities were present. ORS571 GS-affected and NADPH- GOGAT -affected mutant strains were defective in both ammonium assimilation (Asm-) and N2 fixation (Nif-) in culture and in planta ; NADH- GOGAT mutants were Asm- but Nif+. "Bacteroid" GS activity was essentially nil, suggesting symbiotic ammonium export. Physiological studies on effects of glutamine, ammonium, methionine sulfoximine, and diazo-oxo-norleucine on nitrogenase induction in culture implied a regulatory role for the intracellular glutamine pool.

Ammonia↗
PubMed1980

Regulation of Rhizobium nitrogen fixation by the unadenylylated glutamine synthetase I system.

Rhizobium sp. 32H1 glutamine auxotrophs have a complex phenotype: a highly adenylylated glutamine synthetase [GS; L-glutamate:ammonia ligase (ADP-forming), EC 6.3.1.2] I and an undetectable GS II (GS II(-)). They are defective in the adenylylation cascade system for GS I. Prototrophic revertants are of two classes: those (3204 type) which retain the adenylylated GS I phenotype but become GS II(+), and those (3205 type) which acquire a constitutive unadenylylated GS I but remain GS II(-). Like the parent auxotroph, 3204 remains incapable of nitrogen fixation both in culture and in root nodules of Macroptileum atropurpureum. In contrast, 3205 is nitrogenase constitutive. This implies that GS I or associated adenylylation proteins are involved in the control of Rhizobium 32H1 nif gene expression and that GS II is not so involved. Normally, rhizobia fix atmospheric N(2) only during symbiosis and, in so doing, only transiently synthesize nitrogenase. Moreover, whereas wild-type strains export ammonium, constitutive strains can assimilate ammonium produced by nitrogenase. This phenotype allows direct selection of nitrogen fixation-defective mutants in Rhizobium.

Journal Article↗
PubMed1980

Physiological roles of glutamine synthetases I and II in ammonium assimilation in Rhizobium sp. 32H1.

The two glutamine synthetases of Rhizobium sp. 32H1 appear to be structurally and functionally distinct. Glutamine synthetase I was reversibly adenylylated, and its synthesis was repressed only twofold by ammonium. When in the unadenylylated configuration, it was the enzyme which allowed the organism to grow, albeit marginally, on ammonium as a nitrogen source. There is no evidence to suggest that the second enzyme, glutamine synthetase II, is regulated by adenylylation. However, this enzyme was repressed at least 50-fold by even low amounts of ammonium. Glutamine synthetase II does not seem to function in ammonium assimilation but rather in purine biosynthesis.

Adenine↗
PubMed1979

Further examination of presumptive Rhizobium trifolii mutants that nodulate Glycine max.

Two recent reports described the isolation of derivatives of a Rhizobium trifolii strain that had gained the ability to nodulate Glycine max and Vigna radiata and that had demonstrated altered patterns of carbon source utilization, free-living nitrogen fixation, and hydrogen uptake. More extensive characterization of these strains now supports the conclusion that these strains are R. japonicum and are not derived from the putative parent R. trifolii.

Journal Article↗
PubMed1978

Control of ammonium assimilation in Rhizobium 32H1.

The symbiotic, nitrogen-fixing bacterium Rhizobium sp. 32H1 is a specialized ammonium producer during symbiosis. However, during free-living growth, Rhizobium 32H1 assimilates ammonium very poorly. Two pathways of ammonium assimilation exist in enteric bacteria. One is mediated by glutamate dehydrogenase, and the other is mediated by glutamine synthetase-glutamate synthase. The former pathway is altogether inoperative in Rhizobium 32H1; the latter pathway operates at a slow rate and is under strict negative control by ammonium itself. Rhizobium 32H1 glutamine synthetase activity is modulated by both repression-derepression and reversible adenylylation. For a biochemical process lacking an alternative pathway, such a regulatory pattern exacerbates the very process. This suggests that Rhizobium 32H1 restricts its own ammonium assimilation to maximize the contribution of fixed nitrogen to the host plant during symbiosis.

Adenine Nucleotides↗
PubMed1978

Time reporting within a health systems agency.

It is a truism that good management is a prerequisite to successful health planning and that essential to good management is reliable data on what is taking place. Health Systems Agencies (HSAs) have, in general, not taken advantage of management information systems (MIS) which might assist them in obtaining such data, perhaps because of cost or because of fear of burdening professionals with too much record keeping. Yet, performance categories mandated for HSAs provide an excellent base on which to build a simple, flexible MIS. The HSA of Western New York (HSAWNY) has installed an MIS, one component of which is a time reporting system for professional activity. This article discusses the purposes of the system, the analysis leading to its design, the input coding requirements, the output reports, uses for the output, the developmental and operational costs, the difficulties with system development and operation, and suggestions for further action.

Budgets↗