Biomedical subjects

R A Rockerbie

Publications and source records attributed to R A Rockerbie.

12 recordsLinked to original sources

PubMed1995

Computer simulation analysis of blood alcohol.

A mathematical model using an iterative algorithm based on the Widmark formula is used to simulate a continuous spectrum of the expected blood alcohol concentration from start of alcohol dosage to time of specific event in numerical and graphic form. Absorption is based on flexible first order rates with provision for an optional good-fit model of the delaying effect of food. Elimination is based initially on selectable zero order rates followed by first order kinetics at low blood alcohol concentrations. A correlation coefficient of 0.94 was obtained in comparisons of projected to observed blood alcohol concentrations. A measurement of the area under the curve is provided. The software program utilizing the algorithm is suitable for forensic and clinical applications.

Journal Article↗

PubMed1979

Effect of dietary supplementation of vitamin E on serum lipids and lipoproteins in rabbits fed a cholesterolemic diet.

The effect of dietary supplementation of vitamin E on serum cholsterol, triglycerides, lipoproteins and lipoprotein composition was studied in rabbits fed a one per cent cholesterol diet for a period of twelve weeks. Vitamin E supplemented animals were found to maintain significantly lower concentrations of serum cholesterol, triglycerides and very low density lipoproteins (d less than 1.006). The difference in the serum lipid content was mainly in the very low density lipoproteins of d less than 1.006. The possibility of an increased clearance of chylomicron remnants and decreased inhibition of extrahepatic lipoprotein lipase in hypercholesterolemic rabbits as a result of vitamin E supplementation is discussed in the light of the results obtained.

Animals↗

PubMed1979

Gas-chromatographic analysis of patterns of fatty acids of cholesteryl esters and phosphatidylcholine.

We have developed a quick, reliable method for compositional analysis of the C14 to C20 fatty acids of cholesteryl esters, based on the use of a double extraction-solvent system and whisker-walled capillary columns with a new polar phase. Fatty acids of phosphatidylcholine, after separation by thin-layer chromatography, were determined on the same column. Our normal values are in good agreement with those published previously. We found marked changes in the fatty acid composition of both cholesteryl esters and phosphatidylcholine in cord blood serum, in various disease states, and in post-mortem specimens. The previously reported correlation between oleic (18:1) and linoleic (18:2) acids in serum cholesteryl esters was confirmed for normal adults, but no such correlation exists in cord blood specimens. We also found a significant correlation between the relative amounts of fatty acids in cholesteryl esters and phosphatidylcholine for all unsaturated fatty acids and for palmitate in all groups of subjects.

Adult↗

PubMed1978

Effect of specimen storage and preservation on toxicological analyses of urine.

1. The stability of nineteen classes of drugs or their metabolites in urine specimens, for the purpose of toxicological screening has been studied for periods of up to thirty-six weeks. 2. The effect of specimen preservation by refrigation and by the addition of boric acid, chloroform, sodium fluoride, mercuric chloride, and buffers, has been assessed. 3. With the exception of flurazepam, glutethimide, and secobarbital, specimens may be retained at room temperature for periods in excess of six weeks without deterioration. 4. Preservation by the addition of sodium fluoride (5 g/l) followed by freezing, thawing, and filtration significantly prevents specimen deterioration, and may be used as the method of choice.

Central Nervous System Agents↗

PubMed1977

A random survey of drug screening proficiency.

There is little evidence of uniformity of standards of performance as influenced by technical capability and priority of concern for drug-screening proficiency. The objective of this quality-control survey was to illustrate the degree of error associated with toxicological analyses and encourage the use of controls to decrease the possibility of reporting erroneous findings.

Adolescent↗

PubMed1976

Patterned relationships between IgM and blast cells in peripheral blood in a child with acute leukemia.

1. Serum IgM determinations and blast cell counts were performed at regular frequent intervals in a case of acute lymphoblastic leukemia in a child. 2. An inverse relationship tended to exist between the concentration of IgM in serum and blast cells in blood during the course of relapses and remissions. 3. High dosage immunosuppressive chemotherapy caused a marked reduction in serum IgM. 4. A relationship appears to exist between IgM and blast cells which may support the concept that impaired ability to produce antibody to leukemia antigens may be an intrinsic component of this disease.

Blastocyst↗

PubMed1975

Semi-automated colorimetric determination of triglycerides.

1. A semi-automated procedure for the determination of triglycerides based on the manual procedure of Fletcher and the semi-automated procedure of Kessler and Lederer has been developed. 2. Reaction conditions and flow-rates have been optimized to result in a trouble-free procedure which produces 99+ per cent recovery on added recovery tests, and a standard deviation of 4.8 on specimens in the low normal range. 3. An inexpensive scale expansion device is used to increase accuracy by improving chart readability. 4. Analysis of commercial control sera produced results which correlated well with stated values.

Autoanalysis↗

PubMed1974