PubMed HealthSearch

Biomedical subjects

R A Sitdykov

Publications and source records attributed to R A Sitdykov.

3 recordsLinked to original sources

[An electrochemical method of determining peroxidase in saliva].

Electrochemical method has been developed for measuring human salivary peroxidase. Salivary peroxidase levels were measured in donors in various hours of the day, in girls in various periods of the cycle, and in myocardial infarction patients. The results may be used to develop rapid methods for assessment of ovarian functional activity and for prediction of myocardial infarction outcome. Electrochemical analysis productivity is 360 samples per hour, the minimal peroxidase concentration detectable is 5.10(-12) M.

Adult

[A highly sensitive immunoenzyme analysis using electrochemical detection].

The authors have developed a method for improving the sensitivity of electrochemical enzyme immunoassay, based on binding the enzymatic reaction volatile component to a complexone, followed by destruction of the complex in a flow-type amperometric detector. The lowest limit of the measured levels of blood serum antibodies and antigens is 10(-11)-10(-12) M. The method was tried in enzyme immunoassay of the blood serum IgE in patients with asthma and chronic obstructive bronchitis. Reduction of IgE level after therapy was found to be a regular feature; the authors claim that it may be considered as one of the criteria indicating the treatment efficacy.

Adult