Miniature electrophoresis for speed and productivity.
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Animals↗
Biomedical subjects
R A Zeineh
Publications and source records attributed to R A Zeineh.
Measurement of radial immunodiffusion plates with a scanning densitometer.
Measurement of the diameters of the precipitation rings in radial immunodiffusion plates with a scanning densitometer is described. Data obtained by scanning were compared with a conventional visual method of measurement and although the differences were statistically significant, the differences were small and there was no basis for determining which method was a more accurate estimation of concentration. It was concluded that the scanning method, which uses equipment present in most clinical pathology laboratories, is an acceptable alternative method for measuring radial immunodiffusion.
Densitometry↗
The metabolism of transferrin in burned patients.
The metabolism of transferrin was studied in 17 selected burned patients. Transferrin metabolism also was studied in 5 of the patients after healing. The concentration of plasma transferrin in burned patients, measured by radial immunodiffusion, was reduced to 300 mg% for the first week following injury. Thereafter it rose steadily reaching the normal concentration of 350-360 mg% 35 days after injury. Traces of transferrin were present in urine a week after injury. Transferrin was labeled by injecting 14C-glucosamine intravenously following injury and again after healing was complete. Labeled transferrin was isolated from serum by specific immunoprecipitation. The specific activity of transferrin reached a peak 3-6 hr after injury and 9-12 hr after healing. The half-life time was 4.5 days during injury and 9.6 days after healing. The data indicated that both synthesis and catabolism of transferrin were accelerated in injury.
Burns↗
Chromatographic differences in serum amylase among species.
Chromatographic analysis of amylase in human, dog, rabbit, rat and chicken sera were performed and species differences recorded. Iodide, rather than chloride, requirement for optimal activity was demonstrated for one amylase fraction separated from rabbit sera.
Adult↗
Core isoelectrofocusing.
An improved method of isoelectrofocusing is described. A plastic rod is centered in a tube and the lumen is filled with gel. After separation the rod is withdrawn and replaced by staining solution. Staining and destaining are quick and simple. The gel remains stabilized in the tube allowing no distortion or breakage and permitting accurate localization of separated bands.
Blood Proteins↗