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Biomedical subjects

R Allner

Publications and source records attributed to R Allner.

At least 19 recordsLinked to original sources
PubMed1986

[A new method for rapid and simple adsorption of rheumatoid factors from serum].

It is well known that autoantibodies directed against human gamma-globulin, the so called rheumatoid factor (Rheumafaktor), show interference with immunochemical methods. Especially the enzyme-linked immunoassays for detecting the early immune reaction against virus-infections (e.g. rubella, measles, and others) show irregular positive results, if the tests are performed in samples containing IgM rheumatoid factor. We describe a new method for the rapid adsorption and elimination of rheumatoid factor from rheumatoid factor containing serum samples, using a suspension of glutaraldehyde-cross-linked, aggregated IgG in a combined solid- and liquid-phase technique. The bound rheumatoid factor can be subsequently desadsorbed at acid pH so that after washing in neutral-buffer or physiological saline the adsorption-material can be used again. Specific antibodies were not adsorbed.

Adsorption↗
PubMed1985

[Fibrinogen as a source of interference in heterogeneous enzyme immunoassays].

Fibrinogen interferes in heterogeneous enzyme immunological in vitro tests. The effect on the test result is variable and depends on the test principle (competitive binding analysis or sandwich technique). In the tests for digoxin, cortisol, alpha 1-fetoprotein and acid prostate phosphatase, the use of plasma as sample material resulted in varying degrees of interference. In the case of the digoxin determination, using the Enzymun-Test Digoxin, it is possible to abolish interference by fibrinogen by thermal coagulation (plasma is heated at 56 degrees C for 30 min) and removal of the fibrinogen. Alternatively, addition of urea to the antigen-antibody reaction mixture decreases the interference caused by fibrinogen. The added quantity of urea and the time of incubation must be optimized for each sample material.

Acid Phosphatase↗
PubMed1985

[ENT surgery and laboratory medicine--the modern aspects of cooperation].

The value and application of a multiphase screening programme of preoperative laboratory investigations on ENT patients is described and assessed. Appropriate laboratory investigation should be chosen. The methods of investigation should provide information about the patient's general condition, should identify disease which has escaped clinical assessment, which is not connected with the underlying disease, but which could severely handicap a patient undergoing surgery. They are also designed to assist in the medical screening of the general population. The programme includes tests of organ function and of metabolism. The frequency of abnormal laboratory values (less than 2.5% at to longer end of the normal distribution curve or above 5% at the other) was 7.8% for blood sugar, 2.9% for serum creatinine, 4.5% for urea, 11.6% for uric acid in men and 4.8% in women, 9.8% for serum cholesterol, 2.9% for SGOT in men and 20.8% for triglycerides. Tests included in the haemostasiological study were the basic coagulation testing programme (PTT, Prothrombin Time, Thrombin Time and Fibrinogen). Inherited and acquired blood disorders are detected through the full coagulation test programme and assay of the specific coagulation factors and fibrin degradation products. We diagnosed 11 inherited blood disorders in 10,000 patients put through the above screening programme. The problems of diagnosis and therapy of antibody haemophilia are demonstrated by an example of antibody haemophilia of factor VIII.

Blood Chemical Analysis↗
PubMed1984

[Rheumatoid factors and immunoglobulins as interfering factors in a heterogeneous enzyme immunoassay].

Enzymun-Test Digoxin was used to analyse model samples. The matrix of the model samples consisted of either phosphate-buffered 0.15 mol/l sodium chloride solution pH 7.2 with addition of albumin or immunoglobulins, or of various ratios of albumin and immunoglobulins, most of which had been characterized immunoelectrophoretically. Increasing amounts of albumin or immunoglobulins in the model samples caused a simulated increase in the measured amount of digoxin. Apparent digoxin values also showed corresponding false increases when immunoglobulins of certain specificities were increased in the model samples, even when total protein was kept constant.

Digoxin↗
PubMed1979

[Influencing the maternal energy metabolism via infusion of xylite during childbirth (author's transl)].

During parturition, there is an increase in the glucose, pyruvate and lactate levels in the maternal venous blood, as well as an increase in the concentrations of free fatty acids, d-(-)-3-hydroxybutyrate and acetoacetate. Peripartal acetonaemia in the mother can be reduced by infusing solutions which contain xylite. No essential increase in blood lactate levels was seen following in fusion of 0.4 g xylite/kg body weight. The acetone bodies reach the foetal blood via the placenta. It remains to be clarified as to how far they are an important source of energy during the newborn period.

Energy Metabolism↗
PubMed1976

[Serum acid phosphatase levels following prostatic massage (author's transl)].

We find an elevation of serum acid phosphatase levels 5 min after prostatic massage in only 10% of patients with prostatic adenoma. This increase is caused by stored prostatic secretion being pressed into the blood vessels. 60 min later these serum levels decrease. With some other patients a slower increase of phosphatase levels occurs; this increase, however, lasts for hours. This kind of increase is caused by prostatic fluid being forced into the interstitium where it is slowly absorbed. A combination of both kinds leads to a curve with two peaks; this could be demonstrated in two cases. No difference was seen in reaction of total acid phosphatase and prostatic phosphatase levels. The increase of phosphatase levels following prostatic massage was no sign of prostatic carcinoma.

Acid Phosphatase↗
PubMed1976

[Specificity of 3 alpha, 20 beta-hydroxysteroid: nad+ -oxidoreductase (author's transl)].

The enzyme 3 alpha, 20 beta-hydroxysteroid:NAD+ oxidoreductase (EC 1.1.1.53) catalyses the stoichiometric reduction of 20-oxo groups of C-21 steroids by NADH, and the non-stoichiometric oxidation of 3 alpha-hydroxy groups of 5 alpha-androstane derivatives by NAD. The activity of the enzyme towards the most important C-18, C-19 and C-21 steroids of human urine was tested in the presence of NAD and NADH. The rate of reduction of 20-oxo steroids is influenced by substituents at various positions of the steroid skeleton. These studies are intended as the basis of an eventual clinical chemical application.

Androstenes↗
PubMed1975

[A method for quantitative dertermination of 20-keto steroids in urine during the use of a 3 alpha-, 20 beta-hydroxysteroid: NAD oxidoreductase (author's transl)].

A method is described for the quantitative determination of 20-ketosteroids in urine during the application of a 3alpha, 20beta-hydroxysteroid: NAD oxidoreductase. In the presence of NADH H+ the enzyme catalyses in an "equimolar" developing reaction the reduction of 20-keto groups of C-21 steroids giving 20 beta-alcohols. The measurable NADH H+ consumption is a measure for the quantity of those 20-ketosteroids present in the reaction mixture. The 3alpha-hydroxysteroid: NAD oxidoreductase activity of the enzyme does not influence the specificity of the method to determine the 20-ketosteroids because,-under the present extraction and purification conditions-those 3alpha-hydroxy-5alpha-androstan derivatives are not reaching the reaction mixture of determination. The demands towards the criterions of reliability (correctness and exactitude) come to be fulfilled by this method.

Adolescent↗