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Biomedical subjects

R Azuma

Publications and source records attributed to R Azuma.

At least 55 records · Page 3Linked to original sources
PubMed1981

Occurrence of bovine dermatophilosis in the southernmost islands of Japan.

Such cutaneous symptoms as characteristic incrustation and alopecia were noticed in 25 calves of the indigenous Japanese Black breed grazing on subtropical islands in Japan over a period of April, 1978 to February, 1980. These islands were Ishigaki, Kuro, Yonaguni and Tarama belonging to the Sakishima Islands. Microbiological and pathological examination on three of these calves revealed that the calves were affected with dermatophilosis caused by Dermatophilus congolensis. The disease in these calves seemed to be the same as that reported previously in other countries, since it attacked young calves in a humid district with an abundant rainfall. It broke out first on Ishigaki Island and subsequently on the other islands in 3 years. Discussion was made on factors inducing these outbreaks.

Actinomycetales↗
PubMed1979

Occurrence of free ceramides in Bacteroides fragilis NCTC 9343.

The occurrence of free ceramides at high concentrations was demonstrated in the chloroform-methanol extractable lipids of Bacteroides fragilis NCTC 9343. The long-chain bases were isolated from the free ceramides and identified as branched and normal saturated dihydroxy bases with carbon chains consisting of 17, 18, and 19 atoms. The major fatty acid was 3-hydroxy 15-methylhexadecanoic acid. The major molecular species of the ceramides were identified by gas chromatography-mass spectrometry and gas chromatography of the cleaved products as LCB-d-iso17: 0-3-OH iso17: 0 FA, LCB-d-anteiso17: 0-3-OH iso17: 0 FA, LCB-d-iso18: 0-3-OH iso17: 0 FA, and LCB-d-anteiso19: 0-3-OH iso17: 0 FA.

Bacteroides fragilis↗
PubMed1979

Diagnosis of Corynebacterium pyogenes infection in pigs by immunodiffusion test with protease antigen.

A protease antigen was prepared from the culture supernatant of Corynebacterium pyogenes by concentrating with a flash evaporator and ultrafiltration. It was adjusted to the concentration of 32 units by the single radial immunodiffusion with a tentative standard serum. In the immunodiffusion test, the antigen of 4 units reacted enough with sera having an antibody titer ranging from 1 to 128. As a result, it was decided that the antigen of 4 units should be used in the immunodiffusion test for the detection of protease antibody. By the immunodiffusion test, protease antibody was demonstrated in about 35% of 443 sera from pigs collected at random. The antibody titer showed the distribution of 2 peaks. The summits of the two peaks were seen at 4 and 32 of antibody titer, respectively. The valley between the two peaks was seen at 16 of titer. From the result, a diagnostic criterion of the immunodiffusion test was decided provisionally as follows: above 16 of antibody titer is positive, 1 to 8 suspect, and less than 1 negative. On the other hand, protease antibody was demonstrated in sera from 13 of 14 pigs carrying abscesses from which C. pyogenes had been isolated. Its titer was 8 (in 2 pigs), 16 (in 1), 32 (in 3), 64 (in 6), and 128 (in 1). From these results, it was proposed that the immunodiffusion test with protease antigen be used for the diagnosis of C. pyogenes infection in pigs.

Animals↗
PubMed1979

Isolation of Selenomonas spp. from lesions and non-digestive organs of cows, pigs and man.

Selenomonas spp. were isolated for the first time from lesions and non-digestive organs which were apparently normal in 2 cows, 6 pigs, and 1 human being. Identification as Selenomonas was based firstly on electron microscopical observation and secondly on fermentation products. They were divided into 3 major groups by biological properties, as well as by the patterns of these products. It has been confirmed that the habitats of organisms of the genus Selenomonas are generally digestive organs, including the rumen of the ruminant, the cecum of the guinea pig, and the oral cavity of man. The existence of these organisms in lesions and non-digestive organs in such animals and man, however, has been unknown as yet. Moreover, it has been completely unknown about the habitat of these organisms in swine. The findings obtained suggested the possibility of invasion of Selenomonas into other parts than the digestive organ in some animals and the presumable existence of the organism in the swine digestive organs. The role of Selenomonas as a secondary invader into some animals was proposed.

Animals↗
PubMed1977

Precipitating antibody against protease of Corynebacterium pyogenes in pigs.

Antibody in sera from pigs carrying an abscess associated with Corynebacterium pyogenes and healthy pigs was examined by the agar gel diffusion test. In the test, the concentrated culture fluid containing the protease of C. pyogenes was used as antigen. As a result, precipitating antibody was demonstrated in sera from 25 of 30 abscessed pigs and a few of the healthy pigs. When the relationship between precipitating antibody and protease was examined by the immunoelectrophoresis and gel filtration of the concentrated culture fluid, the antibody was shown in the same position as the protease. From the result, it was clear that the precipitating antibody was against the protease of C. pyogenes. All the proteases produced by 27 strains of C. pyogenes of porcine and bovine origin were serologically identical with one another. They were, however, serologically different from those of Staphylococcus aureus, Bacillus cereus, and B. subtilis. In the inhibition test, the proteolytic activity of C. pyogenes was inhibited by the serum of the abscessed pig. It was also inhibited by healthy pig serum. From the results, it seems that the determination of precipitating antibody may be useful for the diagnosis of C. pyogenes infection.

Abscess↗