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Biomedical subjects

R B Forney

Publications and source records attributed to R B Forney.

At least 91 records · Page 5Linked to original sources
PubMedDate not supplied

Extraction, identification and quantitation of succinylcholine in embalmed tissue.

Succinylcholine, a bis-quaternary ammonium compound, was extracted from embalmed tissues as an ion-pair with hexanitrodiphenylamine in methylene chloride. The evaporated ion-pair residue is demethylated with sodium benzenethiolate. The tertiary amine formed is identified and quantitated by gas chromatography/mass spectrometry (GC/MS) utilizing a 25 meter glass capillary column coated with SE 52. Identification is accomplished by retention time and mass spectrometry. Quantitation is performed after the addition of deuterated succinylcholine as an internal standard by focusing the mass spectrometer on m/z 58 (for demethylated succinylcholine) and m/z 62 (for the internal standard). The method is applied to the quantitation of succinylcholine from the embalmed kidney, liver, and muscle of rats injected i.m. with 10-200 mg/kg. After six months of storage, the succinylcholine can still be identified and quantitated with highest concentrations found in the muscle injection site. Concentrations as low as 5 ng/g are easily detected.

Animals↗
PubMedDate not supplied

Use of limited laboratory-acquired therapeutic drug monitoring data in determining individualized drug requirements.

Routinely acquired therapeutic drug monitoring (TDM) data from 220 patients were used to estimate patient-specific pharmacokinetic parameters for the following drugs: aminoglycoside antibiotics (gentamicin and tobramycin), digoxin, theophylline, carbamazepine, procainamide, phenobarbital, and quinidine. A microcomputer based set of algorithms operating on two relatively unconstrained TDM values estimated pharmacokinetic parameters with which future TDM levels were forecast. Mean prediction errors (mpe) and root mean squared errors (rmse) were used as measures of predictive performance. Values of mpe deviated from zero by less than one microgram per l for digoxin and by less than one microgram per l for all other drugs. Values of rmse were also small when viewed in the context of the respective therapeutic plasma concentration ranges.

Adolescent↗