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Biomedical subjects

R B Mitchell

Publications and source records attributed to R B Mitchell.

47 records · Page 3Linked to original sources
PubMed1981

Hydrocortisone effects on nucleic acid and nuclear protein content and protein metabolism in aging WI-38 cells.

Confluent late passage WI-38 human diploid fibroblast cultures grown in the presence or absence of hydrocortisone for fifteen population doublings were measured cytochemically for DNA, nuclear RNA, basic nuclear protein and acidic nuclear protein content during a 12-hour interval following a medium-change stimulation. Radioisotope tracer studies were utilized to assess the effects of prolonged hydrocortisone treatment on protein synthesis and degradation in aging confluent and logarithmically growing cultures. Hydrocortisone-treated cultures exhibited an increased Feulgen--DNA stainability, no difference in basic nuclear protein content, a significantly larger initial decrease followed by an increase in acidic nuclear protein content, and elevated levels of nuclear RNA as compared to untreated controls. Hydrocortisone treatment also resulted in increased incorporation of leucine into protein and decreased breakdown of pre-labeled protein in both confluent and logarithmically growing cultures. These results indicate that many of the metabolic changes associated with proliferation are stimulated by prolonged hydrocortisone treatment of late passage WI-38 fibroblast cells.

Cell Division↗
PubMed1981

Age-related changes in brain RNA of noise stressed mice.

Correlative data were obtained on RNA levels of cerebrocortical neurons, adrenal fasciculate cell RNA content and serum corticosterone levels in 10, 40 and 100 week old male C57BL/6J mice immediately following a 30 interval of continuous noise stimulation (100 dB re 2 x 10(-5) N/m2, 0.35-20 kHz) and at 1 hr and 4 hr recovery intervals. Quantification of cellular RNA levels entailed use of scanning-integrating microdensitometry of azure B stained tissue sections. Hormonal analyses were done by radioimmunoassay. Noise stimulation induced a mild to moderate adrenocortical activation and a moderate elevation of serum corticosterone levels in all age groups. Noise exposure also resulted in an increase in the RNA content of cerebrocortical neurons. However, maximal neuronal RNA levels proved to be considerably lower (32-36%) in 100 week old mice as compared to both 10 and 40 week old mice during the post-exposure intervals. The overall data support the existence of a lower neuronal capacity for sensory induced RNA synthesis in 100 week old mice. The data also suggest that age dependent differences in neuronal responsiveness are not directly related to the extent of adrenal RNA activation or to a lower availability of circulating glucocorticoids.

Adrenal Cortex↗
PubMed1976

Cytophotometric identification of tetraploid Purkinje cells in young and aged rats.

DNA content of cerebellar basket and Purkinje cells of four month, one year, and two year old albino rats was determined by Feulgen cytophotometry. DNA content per nucleus remained essentially constant during aging although there was a slight shift to lower Feulgen-DNA values occurring in the one year age group. Purkinje cells of all three age groups were found to contain tetraploid amounts of DNA as compared to diploid basket cells.

Aging↗
PubMed1966

Microorganisms of the upper atmosphere. I. Instrumentation for isokinetic air sampling at altitude.

An aircraft-mounted air-sampling system for the collection of microorganisms from the upper air is described. The system is highly suitable for use in upper air sampling from an aircraft because (i) it is an isokinetic system, (ii) it is simple to operate, (iii) it employs a dry filter, (iv) the volume sampled can be easily determined, and (v) it operates efficiently at all altitudes and ambient temperatures.

Air Microbiology↗
PubMed1966

Microorganisms of the upper atmosphere. II. Microorganisms in two types of air masses at 690 meters over a city.

The viable micropopulation found, at altitude over a city, in a land air mass was significantly higher than that found in a marine-influenced air mass. The percentage distribution of bacteria and fungi was approximately equal in both types of air masses. This indicates that, under the conditions of the experiment, the marine air mass was influenced by the land area over which it traveled during passage from its source to the sampling area. Activities taking place within the city significantly increased the micropopulation at altitude. This increase was quantitatively so small that it was not identifiable when the micropopulation moving into the city was high-as in a land air mass-but was recognizable when the micropopulation was low-as in a marine-influenced air mass. The modification of the micropopulation at altitude by temperature inversions was shown.

Air Microbiology↗