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Biomedical subjects

R B Staigmiller

Publications and source records attributed to R B Staigmiller.

At least 37 records · Page 2Linked to original sources
PubMed1987

Pregnancy rates of beef heifers bred either on puberal or third estrus.

The objective of this study was to determine if pregnancy rates (PR) differed between beef heifers bred to fertile bulls on either their puberal (E1, n = 89) or third (E3, n = 67) estrus. Heifers were obtained from two lactations (Manhattan, L1; and Miles City, L2), and the experiment was conducted at Miles City. Heifers were assigned randomly within location to either E1 or E3. Heifers were fed to gain .56 kg.head-1 X d-1 and observed twice daily for estrus. After exhibiting first estrus (puberty) and breeding, each heifer in E1 was palpated rectally on d 6, 9 and 12 +/- 1 d (estrus = d 0) for the presence of a corpus luteum, and a venous blood sample was collected for assay of progesterone by radioimmunoassay. Heifers in E3 were palpated and bled on the same schedule as heifers in E1 after first estrus and after being bred to a fertile bull at third estrus. Pregnancy rates were determined by rectal palpation at approximately 38 d post-breeding. Location of origin did not affect (P greater than .10) weight at puberty or weight at breeding; however, heifers from L1 were younger (P less than .05) than heifers from L2 at puberty and breeding. Pregnancy rates were 57 and 78% for heifers in E1 and E3, respectively (P less than .05). Weight at breeding did not influence (P greater than .10) pregnancy rates. The probability of heifers in E1 becoming pregnant increased (P less than .05) with increasing age, while age was not a factor (P greater than .10) for heifers in E3. These results indicated that fertility of puberal estrus in beef heifers is lower than third estrus. Higher fertility of third estrus may be related to maturational changes associated with cycling activity.

Animals↗
PubMed1987

Progesterone concentrations in beef heifers bred at puberty or third estrus.

Peripheral serum progesterone concentrations were evaluated in beef heifers following breeding collected on d 6 +/- 1, 9 +/- 1 collected on d 6 +/- 1, 9 +/- 1 and 12 +/- 1 (estrus = d 0) after the puberal estrus of all heifers and after the third estrus of E3 heifers. Progesterone concentrations were higher (P less than .05) for heifers in E1 compared with heifers in E3 on d 6, 9 and 12 after breeding to a fertile bull. Progesterone concentrations on d 6, 9 and 12 did not differ (P greater than .10) between pregnant heifers in E1 and E3; however, non-pregnant heifers in E1 had higher (P less than .05) concentrations of progesterone compared with non-pregnant heifers in E3 on each day. Concentrations of progesterone did not differ (P greater than .10) between non-pregnant heifers in E1 and heifers of E3 during their puberal cycle. Pregnant heifers in E1 and E3 had higher (P less than .05) concentrations of progesterone on each day than non-pregnant heifers in their respective treatments. There were no interactions (P greater than .10) between treatment, pregnancy status and day-of-estrous cycle for concentrations of progesterone. Results of this study indicated that luteal function differed between heifers that failed to conceive at their puberal estrus and heifers that failed to conceive at third estrus. However, concentrations of progesterone did not differ between heifers that conceived at puberal or third estrus. The relationship of changes in luteal function from the puberal through the third estrous cycle and pregnancy is not clear.

Animals↗
PubMed1986

Fetal growth of beef calves. I. Effect of prepartum dietary crude protein on birth weight, blood metabolites and steroid hormone concentrations.

Fifty-nine crossbred heifers (427 kg) bred to one Hereford sire were randomly assigned at 75 d prepartum to two diets. Heifers were individually fed, and diets were isocaloric but contained either a low (LP = 81% NRC, .56 kg/d) or high (HP = 141% NRC, .98 kg/d) level of crude protein. Jugular vein cannulae were inserted into 16 LP and 16 HP heifers at 10 prepartum. Daily preprandial blood samples that were collected until parturition were analyzed for serum estradiol-17 beta (E2), progesterone (P4), glucose (G) and urea nitrogen (UN). Heifers fed LP gained slower than HP-fed heifers before calving (.73 vs 1.02 kg/d; P less than .01); immediate post-calving weights and condition scores were 418 vs 444 kg (P less than .01) and 5.4 vs 6.1 (P less than .01; LP vs HP, respectively). Calf birth weights (35.3 vs 36.1 kg), average calving difficulty score (1.6 vs 1.6) and percent assisted births (35.5 vs 35.7%) did not differ (P greater than .10; LP vs HP, respectively). Prepartum concentrations of UN (6.2 vs 13.5 mg/dl) and G (52.9 vs 58.2 mg/dl) were lower (P less than .05) and P4 (5.94 vs 4.26 ng/ml) was higher (P approximately equal to .07) in LP heifers. Prepartum concentration profiles were related to calving difficulty score (CD, 1 = no assistance to 3 = hard pull) for E2 (CD1 vs CD2 + CD3, P less than .01; CD2 vs CD3, P approximately equal to .01), P4 (CD1 vs CD2 + CD3, P less than .05), G (CD1 vs CD2 + CD3, P less than .05) and UN (CD2 vs CD3, P less than .05). After calving, all dams were maintained together on pasture and supplemented with alfalfa hay and grain mix until adequate range forage was available to maintain weight gains. Dams that were fed LP prepartum gained faster than HP dams during this period (.49 vs .15 kg/d; P less than .01). Prebreeding weights (443 vs 453 kg; LP vs HP) and condition scores (5.1 vs 5.1) did not differ, nor was the postpartum interval affected (44 vs 40 d; LP vs HP). There was no effect of dietary protein on dystocia or postpartum interval, although there were diet-induced differences in body weight and condition of the dams at calving. Results indicate that differences in prepartum profiles of serum steroid hormones and metabolites may be related to dystocia, in addition to relative fetal oversize.

Animals↗
PubMed1986

Fetal growth of beef calves. II. Effect of sire on prenatal development of the calf and related placental characteristics.

Fifteen Hereford and 47 crossbred heifers were allotted by breed and body weight to be artificially inseminated to one of two Angus sires selected for progeny birth weights (L = low; H = high). Forty-two of the heifers were randomly assigned to be slaughtered at 200, 215, 230, 245 or 260 d of gestation for measurement of fetal and placental characteristics. Twenty heifers were allowed to go to term and five calves from each sire group were randomly assigned to be euthanized and dissected within 24 h after birth. Sire differences in birth weight (BW) and dystocia score (32.9 vs 35.4 kg; 1.8 vs 3.1, L vs H sires, respectively) existed (P less than .01), and there was a sire effect (P less than .01) for fetal calf weights (FW) and eviscerated calf weights (EW). However, there was a sire X calf sex interaction for BW (P less than .05), EW (P less than .01), FW (P less than .01), femur length (P less than .05), heart weight (P less than .05), kidney weight (P less than .01) and pituitary weight (P less than .01). Weight differences suggested these interactions were a result of the relationship of the organ weights to fetal body weights and the interaction effects on calf weights resulted from limitations in the maternal environment which restricted growth of H-sired male calves in utero. Sire X fetal age interaction effects were all nonsignificant (P greater than .10) except for cerebrum weight. This finding indicates that fetus and calf growth rates were parallel for the L and H sires. A sire effect was found for biceps (P less than .01) and liver weights (P less than .01), but not for cerebrum weights (P greater than .10). Increasing weight due to fetal age was attributed to hypertrophy for the cerebrum (P less than .05) and liver (P approximately equal to .01), while the biceps increased (P less than .05) by both hypertrophy and hyperplasia, as determined from deoxyribonucleic acid and protein analyses. All measured fetal organ weights except heart, when expressed as a ratio with EW, decreased (P less than .05) with increasing fetal age. Brain (cerebrum + cerebellum):liver weight ratios were higher (P less than .01) in L-sired calves (.32 vs. .28) than in H-sired calves. Total placentome weight (b' = 91; P less than .01) and placental fluid volume (b' = .32; P less than .01) were highly associated with FW, accounting for 84% of the variation in FW.(ABSTRACT TRUNCATED AT 400 WORDS)

Animals↗
PubMed1985

Sexual development in beef bulls following zeranol implants.

Two trials were conducted to study the effect of zeranol implants on growth and sexual development of bull calves. Trial 1 compared the effects of implanting with 72 mg of zeranol at 48 d of age (branding), at 215 d of age, or at both times with nonimplanted control bulls. Implanting at branding resulted in decreased scrotal circumference, testicle weight and proportion of bulls that could produce an ejaculate at 14 mo of age (P less than .01). Implanting at 215 d of age had no effect on any of these traits. Growth rate was not increased by implanting at either time but was decreased (P less than .02) in animals implanted at both times when compared with control bulls. In trial 2, both bulls and steers were implanted with zeranol and compared with nonimplanted control bulls and steers. Thirty-six-milligram implants were given at 21, 103, 260 and 343 d of age. Scrotal circumference, testicle weight and serum testosterone concentrations decreased (P less than .01) and the occurrence of penis abnormalities increased (P less than .01) in implanted bulls compared with control bulls. By the time of slaughter, however, testosterone concentrations were equal in control and implanted bulls; and the difference in scrotal circumference was diminishing. This is interpreted as evidence that as the bulls get older, they can overcome the effect of the implants. Carcass weights were heavier in implanted steers than in control steers but were lighter in implanted bulls than in control bulls (P less than .02). Carcasses of implanted bulls had higher quality scores and more marbling than control bulls, but carcasses of implanted steers had lower quality scores and less marbling than control steers (both interactions, P less than .01). Implanting bulls with zeranol at an early age resulted in restricted sexual development but not in total sterility. Repeated zeranol implants throughout the growing and finishing phase enhanced carcass quality in bulls slaughtered at 14 to 16 mo of age.

Animals↗
PubMed1985

Nonpuberal estrus and mature cow influences on growth and puberty in heifers.

This experiment had two objectives. The first was to test a hypothesis that the presence of mature cows can influence the growth rate and physiological maturation rate of growing heifers. The second objective was to define a phenomenon termed nonpuberal estrus (NPE). Nonpuberal estrus in a prepuberal heifer is a behavioral estrus that is not followed by ovulation and formation of a corpus luteum. Two years of data were collected. All heifers were crossbred and were sired by Hereford, Charolais and Tarentaise bulls in yr 1 (n = 153) and by the same three breeds plus Jersey, Shorthorn, Brahman and Longhorn in yr 2 (n = 207). All heifers were born in the spring and weaned in October when approximately 180 d old. After weaning, heifers were assigned at random (within breed of sire) to control or mature cow (MC) treatment pens. Control pens contained 25 or 26 heifers and a sterile marker bull, while MC pens contained 25 or 26 heifers and a sterile marker bull plus four nonpregnant, mature cows. The presence of the mature cows did not (P greater than .10) affect rate of gain or yearling weight. Sire breed did (P less than .01) influence weight on test and average daily gain during the test in both years and affected (P less than .01) yearling weight during yr 2 but not (P greater than .10) during yr 1. A sire breed X treatment interaction effect on puberal traits was significant (P less than .01) in yr 1 but not in yr 2.(ABSTRACT TRUNCATED AT 250 WORDS)

Age Factors↗
PubMed1984

Relationship between pituitary responsiveness to Gn-RH and number of Gn-RH-binding sites in pituitary glands of beef cows.

Changes in the ability of Gn-RH to induce gonadotrophin release with time after synchronization of oestrus was determined in 4 groups of 6 cows each. Cows were given Gn-RH at 40-min intervals for 6 h beginning at -24, 0, 18 or 36 h (time 0 = removal of progestagen implant). Changes in concentration (ng/ml) of serum LH after Gn-RH averaged 2.9, 6.2, 6.4 and 33.4, whereas serum FSH averaged 25.7, 35.8, 35.8 and 97.3. Thus the responsiveness of the pituitary to Gn-RH had increased by 36 h after implant removal. Other groups of cows subjected to the same synchronization scheme were slaughtered at 0 h, 24 h or at various times after onset of oestrous behaviour. Gn-RH binding to crude pituitary membrane preparations was assessed. There was no apparent change in the affinity constant of Gn-RH-binding sites with time after synchronization. The number of Gn-RH-binding sites remained unchanged until the period of oestrus when a significant decline with time was detected. We conclude that the increase in pituitary responsiveness to Gn-RH that occurs before the preovulatory gonadotrophin surge was not directly associated with changes in number or affinity of pituitary Gn-RH-binding sites in crude pituitary membrane preparations.

Animals↗
PubMed1984

Negative feedback control of luteinizing hormone secretion in prepubertal beef heifers at 60 and 200 days of age.

Prepubertal beef heifers at 60 and 200 d of age, born in the fall or spring, were assigned randomly to one of three treatment groups: (1) intact = 1; (2) bilateral ovariectomy (OVX); or (3) OVX plus estradiol-17 beta(E2) administered in silastic implants (OVX + E2). Luteinizing hormone (LH) was measured in serum samples collected at 20-min intervals for 4 h from heifers on -1, +7, +21, +35 and +49 d after OVX. Luteinizing hormone concentrations increased in the serum by 7 d after OVX in heifers at both 60 and 200 d of age (P less than .001; time X treatment). Prior to OVX, the LH patterns were characterized by low levels and infrequent episodic pulses. By 49 d after OVX, the mean LH concentrations increased and the pattern changed to one of rhythmic LH pulses with a periodicity of 1 h (P less than .001; time X treatment). Estradiol-treated OVX heifers did not exhibit a postovariectomy rise in serum LH concentrations. Serum E2 concentration 49 d after OVX in OVX heifers was threefold greater than in 1 or OVX heifers, thus demonstrating that E2 exerted negative feedback on pituitary LH secretion in prepubertal heifers. There was no measurable difference in serum E2 concentrations between I and OVX heifers; however, the contrast in the concentration and pattern of serum LH between the two groups was dramatic and suggested gonadal factors in addition to E2 are involved in controlling LH secretion.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1983

Estimating the impact of management decisions on the occurrence of puberty in beef heifers.

Age and weight at puberty in beef heifers were hypothesized to be jointly dependent random variables with the joint distribution conditional upon values of exogenous variables such as breed, date of birth and postweaning rate of gain. A statistical model was specified that tested the joint dependency hypothesis and measured the impact of variation in the explanatory variables on puberty. Model parameters were estimated by two-stage least-squares procedures from data on 556 beef heifers: 190 raised at the Northern Agricultural Research Center (NARC), Havre, Montana and 366 raised at the Livestock and Range Research Station (LARRS), Miles City, Montana. Results did not support the hypothesis that age and weight at puberty were jointly dependent; weight at puberty did depend upon age at puberty, but age at puberty did not depend upon weight at puberty when postweaning level of nutrition was known. Thus, the appropriate probability distribution was a conditional univariate probability distribution on age. Results were consistent with past research in that breed and breed-cross differences existed, and heifers heavier at weaning and fed higher levels of postweaning nutrition reached puberty at an earlier age and heavier weight.

Age Factors↗
PubMed1983

Growth and reproductive traits in beef heifers implanted with zeranol.

Weaned replacement heifers were implanted with zeranol at 8 and 11 mo of age in two trials to evaluate growth and reproductive traits. Approximately 100 beef heifers were used in each trial, and the heifers were divided into categories of heavier than average (H) and lighter than average (L) weaning weight. Weight categories were further divided into control (HC and LC) and zeranol-implanted (HI and LI) groups. Heifers were kept in drylot from weaning to just before the start of the breeding season and were fed to reach a predetermined body weight by the start of the breeding season and were fed to reach a predetermined body weight by the start of the breeding season. Rates of gain in the drylot were greater in implanted than in control heifers in both trial 1 (.53 vs .48 kg/d; P less than .09) and trial 2 (.70 vs .63 kg/d; P less than .01). Pelvic areas were greater (P less than .01) in implanted than in control heifers of both trial 1 (175 vs 159 cm2) and trial 2 (175 vs 164 cm2). This increase in pelvic size was still present at the end of the summer pasture period, which followed the drylot period. Zeranol had no major effect (P greater than .10) on age of weight at puberty. Fall pregnancy rate was 16% lower in implanted heifers than in control heifers in trial 1 (62 vs 78%, P = .08) but did not differ in trial 2 (88 vs 87%, I and C, respectively).

Animals↗
PubMed1982

Relationship of growth and puberty in beef heifers fed monensin.

An experiment was designed to determine the effects of dietary monensin on age and weight at puberty in Bos taurus crossbred beef heifers. One hundred and forty heifers were allotted to two weight classes: heavy (H) and light (L) groups, consisting of heifers above and below the average weaning weight, respectively. Heifers within each weight class were assigned to one of three diets during a 203-d winter feeding period: (1) R, 80% roughage: 20% concentrate; (2) M1, R diet plus monensin (200 mg/head daily) with feed intake restricted to produce average daily gains (ADG) similar to R and (3) M2, R diet plus monensin (200 mg/head daily). ADG was higher (P less than .001) for M2 heifers than for R and M1 heifers, which performed similarly. Age and weight at puberty were similar across treatments in L heifers. However, in the H group, HM1 and HM2 heifers were younger (P less than .07) at puberty than HR heifers and this difference was not removed by covariate adjustment of either ADG or body weight. Weigh at puberty was greater (P less than .01) for HM2 heifers than for HM1 or HR heifers; however, this difference was removed by covariate adjustment of either ADG or body weight. Ninety-one percent of LM2 heifers and 100% of all other groups had reached puberty before the breeding season. Pregnancy rates did not differ significantly between treatment groups. Supplementation of a high roughage diet with monensin decreased age at puberty of heifers in the heavy weight class and this decrease was not due to increased body weight or ADG. Perhaps heifers with above average weaning weight possess a greater inherent growth potential than heifers below the average weaning weight and hence are more capable of utilizing monensin to an advantage.

Acetates↗
PubMed1981

Synchronization of estrus in gilts with allyl trenbolone: fecundity after natural service and insemination with frozen semen.

The synthetic progestogen, allyl trenbolone, was fed daily to 60 gilts at 15 mg/gilt for 18 days. Sixty-eight gilts that came into estrus the same week as treated gilts served as controls. Gilts were checked for estrus twice daily and were artificially inseminated with frozen semen or bred by natural service. All but two treated gilts returned to estrus between 4 and 7 days after withdrawal of allyl trenbolone (mean = 5.6; SD = .82). The farrowing rate among gilts inseminated with frozen semen was significantly lower than that among gilts bred by natural service (52.5 vs 89.6%). The farrowing rate among synchronized gilts was similar to that among untreated gilts (70.7 vs 73.5%). Average total and live litter sizes at birth and litter size at weaning were significantly smaller for untreated gilts inseminated with frozen semen than for synchronized gilts inseminated with frozen semen or those of untreated gilts bred by natural service. Average litter size for synchronized gilts bred by natural service was not significantly larger than that for synchronized gilts inseminated with frozen semen or that of untreated gilts bred by natural service.

Animals↗
PubMed1979

Multiple linear and nonlinear regression analyses of factors causing calving difficulty.

Calving difficulty (CD) was analyzed as the dependent variable from observations on 592 first-calf, 2-year-old heifers that were either Angus X Hereford (AH), Charolais X AH or Simmental X AH. Independent variables were birth weight (BW), pelvic area (PA), BW.(33), PA.(5), cow weight (CW), cow condition score (CS) and sex of calf (S). CD was analyzed as score (1 = no difficulty through 4 = extreme difficulty) CD-I, % CD (1 vs 2 + 3 + 4 , CD-II) or % high CD (1 + 2 vs 3 + 4 , CD-III). R(2) values from CD-II and CD-III multiple correlation (R) analyses were consistently lower (.10 to .13) than from the CD-I analysis. When only BW and PA were included in the model, R(2) values for CD-I were .36, .39 and .39 for the linear, quadratic and cubic analyses, respectively. When BW, PA, CW, CS and S were included in the model, the R(2) values for CD-I were .40 and .42 for the linear and quadratic analyses (cubic effects were not tested). In the linear analysis with BW.(33) and PA.(5), the R(2) for CD-I was .36. In all analyses, the order of importance of the factors studied was BW and PA, with a much lower contribution from CW and S. CS did not account for a significant increase in R(2) in any analysis. CD remained below 50% whenever the PA BW ratio exceeded 8.4. This ratio is a convenient practical method to relate PA and BW to CD. Conclusions are that the linear effects of BW and PA account for the majority of the identified variation in CD (R(2)) and that small increases in R(2) can be obtained by adding CW, CS and S to the model and/or by expanding the model to include quadratic effects.

Journal Article↗