PubMed Health⌕ Search

Biomedical subjects

R Baba

Publications and source records attributed to R Baba.

45 records · Page 3Linked to original sources
PubMed1981

Physical and functional mapping of Tn2603, a transposon encoding ampicillin, streptomycin, sulfonamide, and mercury resistance.

A map of cleavage sites for restriction endonuclease EcoRI, BamHI, HindIII, and SalI on Tn2603, a transposon encoding resistance to ampicillin, streptomycin, sulfonamide, and mercury, was constructed by an analysis of restriction cleavage patterns of plasmid pMK1.::Tn2603 and its deletion derivative. By cloning the fragments generated from pMK1.::Tn2603 with these restriction endonucleases to a pACYC184 plasmid vehicle, the regions necessary for expression of resistance were located on the restriction cleavage map of Tn2603. Ampicillin, streptomycin, and sulfonamide-resistance genes were mapped in a cluster on the region between the center and the right and the mercury-resistance gene was located to the left of the map. The final functional map of Tn2603 was compared with those of Tn4 and Tn21 and the evolutional relationships between them were discussed.

Chromosome Mapping↗
PubMedDate not supplied

Inhalation of low-dose nitric oxide to evaluate pulmonary vascular reactivity in children with congenital heart disease.

The objective of this study was to investigate the efficacy of low-dose nitric oxide (NO). The study used fifteen consecutive Japanese preoperative patients (7 males and 8 females) with congenital heart disease and pulmonary hyptertension (mean pulmonary arterial pressure >30 mmHg), 6 of these patients had Down's syndrome. Hemodynamic measurements were taken in room air, 100% oxygen, 5 and 40 parts per million NO (NO5 and NO40) by inhalation. The differences between two observations within the same group were determined by the two-tailed paired t-test. A pulmonary vascular resistance (Rp) regression curve was constructed by using linear regression analysis. The percentage change in pulmonary arterial pressure per systemic arterial pressure (Pp/Ps) with NO40 (Pp/Ps-40) exceeded that of Pp/Ps-5 (p < 0.0001). The percentage change for the Rp with NO40 (Rp-40) was larger than that for the Rp-5 (p = 0.0003). The percentage change of Pp/Ps-5 and that with oxygen were similar (p = 0.266). The relationship between Rp-5 and Rp-40 was linear. In conclusion, the effects of NO5 were equivalent to 100% oxygen but less than NO40. NO5 should initially be used to test pulmonary reactivity. If there is no response, patients should still be given NO40.

Administration, Inhalation↗
PubMedDate not supplied

The oxygen uptake efficiency slope and its value in the assessment of cardiorespiratory functional reserve.

Oxygen uptake efficiency slope is a new index of cardiorespiratory functional reserve derived from the logarithmic relation between oxygen uptake (VO(2)) and minute ventilation during incremental exercise. The oxygen uptake efficiency slope represents how effectively oxygen is extracted and taken into the body from the air, and then ventilated. The physiologic backgrounds of the index are based on: 1) the development of metabolic acidosis that is controlled by the distribution of blood to the skeletal muscles; 2) the physiologic dead space that is affected by the perfusion to the lungs; and 3) arterial carbon dioxide partial pressure. One of the greatest advantages of the oxygen uptake efficiency slope is that it can be calculated by exercise data of submaximal levels. Another advantage is that it is mathematically determined from a set of gas analysis data and, therefore, is completely an objective measurement. Moreover, the oxygen uptake efficiency slope is shown to be highly reproducible. Clinical applications of the oxygen uptake efficiency slope have been reported initially from a population of pediatric patients with heart disease, then from adult patients with chronic heart failure, and finally from elite endurance athletes. Further applications are expected with the use of this sophisticated index. (c)2000 by CHF, Inc.

Journal Article↗