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Biomedical subjects

R Baur

Publications and source records attributed to R Baur.

At least 37 records · Page 2Linked to original sources
PubMed1986

[Normal proximal ureter of the human. Methodology of histomorphometry and results].

A reliable method of histomorphometric evaluation was developed for comparative investigations on cross sections of normal and pathologically altered human ureters. The reproducibility of the measurements with our rating method was good. In addition to the total area of proximal ureteral cross sections, the proportion of lumen, epithelium, submucosa, and muscularis was determined. Connective tissue, musculature, and edema in the ureteral wall were differentiated by staining. This differentiation, like the counting of inflammatory infiltrates, was done in predefined sectors. Measuring results were summarized in one table. The procedure was illustrated in two diagrams.

Connective Tissue↗
PubMed1986

[Proximal ureter of the human in reflux nephropathy and in chronic, nonreflux-induced pyelonephritis. Histomorphometric study].

The total area and the proportion of lumen, epithelium, submucosa and muscularis as well as connective tissue, musculature, and edema were determined by histologic and morphometric evaluation in cross sections of 30 segments of undilatated proximal ureter. The conditions in 8 reflux ureters from children were almost identical to those in 12 reflux ureters from adults. No statistically significant increase in proliferation of connective tissue within the submucosa and muscularis could be established. The primary difference between the reflux ureters and the 10 proximal ureters in reflux-unrelated chronic pyelonephritis was extensive inflammatory edema in the submucosa and muscularis in the latter. Pathophysiologic and pathogenetic aspects are discussed.

Adult↗
PubMed1984

Biological activity of lymphocytotoxic antibodies in Graves' disease and Hashimoto's thyroiditis.

Sera of 48 patients with Graves' disease (GD) and 23 with Hashimoto's thyroiditis (HT) were tested for lymphocytotoxic (LCT), granulocytotoxic (GCT) and monocytotoxic (MCT) activity. In GD, 12 patients (25%) had cold-reacting LCT and 13 patients (27%) had warm-reacting LCT. LCT were cytotoxic to both B and T cells but the majority of sera with cold-reacting LCT and eluates from lymphocytes were more cytotoxic to B lymphocytes. Warm-reacting LCT were directed exclusively against B cells. LCT did not correlate with peripheral lymphocyte counts, antithyroglobulin or antimicrosomal antibodies, sex, age, pregnancies, thyroid status or medication. However the mean duration of the disease was 15 months in LCT positive group and 55 months in LCT negative group (p less than 0.01). Weak GCT were found in 8 of 35 sera (23%). Six of 33 sera (18%) contained cold-reacting MCT and 9 (27%) had warm-reacting MCT. Some cytotoxins were directed against several types of cells as evidenced by cytotoxicity of eluates from lymphocytes against PMN and/or monocytes. Of 23 patients with HT, 11 (48%) had cold-reacting LCT. None had warm-reacting LCT. Sera and eluates from lymphocytes showed predominant cytotoxicity toward B cells. No correlation to the presence of antibodies, sex, age, pregnancies, thyroid status or medication was detected. Four of 23 sera had weak cold-reacting GCT, 5 had cold-reacting MCT which killed on average 31% of monocytes and 4 had weak warm-reacting MCT. Twelve of 22 sera from GD and HT had cytotoxic activity against thyroid cells (TCT). TCT correlated with LCT at p less than 0.05.(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗
PubMed1982

[5-Methylcytosine content in erythroleukemic cells of rats after induction with dimethylsulfoxide].

Erythroleucemic cells of rats were induced by DMSO for the terminal erythroid differentiation. The base composition and the 5 MC content of the DNA were analyzed by HPLC and a significant difference between non-induced and induced cells was not found. The analysis of labelled DNA by the restriction enzymes Hha I, Hpa II, and Msp I did not result in a different pattern before and after the induction. These results suggest that the 5 MC content of the DNA does not change during the induced terminal erythroid differentiation.

5-Methylcytosine↗
PubMed1980

Riboflavin synthases of Bacillus subtilis. Purification and properties.

A variety of Bacillus and Clostridium strains were found to contain two forms of riboflavin synthase which can be easily separated by density gradient centrifugation. The fast sedimenting species accounts for 12 to 44% of the total riboflavin synthase activity in the strains analyzed. Both riboflavin synthases were purified to apparent homogeneity from cell extracts of a genetically derepressed mutant of Bacillus subtilis. The specific activities of the pure proteins were 50,000 nmol mg-1 h-1 (light enzyme) and 2,000 nmol mg-1 h-1 (heavy enzyme). The sedimentation velocities (S20,w) were 4.1 and 26.5 S, respectively. Light riboflavin synthase showed a molecular weight of 70,000 in sedimentation equilibrium experiments. Sodium dodecyl sulfate polyacrylamide gel electrophoresis showed a single band corresponding to a molecular weight of about 23,500. Thus the enzyme appears to consist of three identical subunits (alpha type). Heavy riboflavin synthase has a molecular weight of 1,000,000 as shown by sedimentation equilibrium analysis. The protein appears to consist of 2 or 3 alpha subunits and approximately 60 beta subunits. A fragment apparently identical with light riboflavin synthase can be obtained from the heavy enzyme by mild dissociating treatment.

Bacillus subtilis↗
PubMed1979

Methylation of DNA in developing embryos of the sea urchin Psammechinus miliaris.

Embryos of the sea urchin Psammechinus miliaris have been labelled after fertilization with [6(-3)H]uridine and cultured in filtered sea water. 32-cell, blastula, gastrula and pluteus stages were harvested. The DNA from these embryos was purified and hydrolyzed and the nuclear bases were analyzed by means of high performance liquid chromatography. The ratios of 5-methylcytosine and cytosine demonstrate that the concentrations of 5-methylcytosine are essentially the same in the developmental stages examined (gamma = 95%), which contradicts the hypothesis that methylation of DNA plays a role in cell differentiation.

Animals↗