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Biomedical subjects

R Bhatnagar

Publications and source records attributed to R Bhatnagar.

9 recordsLinked to original sources
PubMed1992

Rat glioma cell lines C6 and 9L synthesize type 1 collagen in vitro.

Glial cell lines (C6, a glioma and 9L, a gliosarcoma) grown in vitro produce type 1 collagen which is detectable in the extracellular matrix by immunocytochemistry. Northern blot analysis using a cDNA specific for the proalpha2 (I) chain of procollagen indicates the presence of a single transcript with an apparent size of 4.8 kb in the C6 cell line, whereas two transcripts with apparent sizes of 5.8 and 4.8 kb are visualized in the 9L cells. The stimulatory effect of ascorbic acid on collagen production is detectable by a 20-27% increase in the concentration of hydroxyproline in the culture medium from the two glioma cell lines. Therefore these glioma cell lines provide a valuable model system for comparative investigations on the regulation of type 1 collagen synthesis by nonmesenchymal cells of neuroepithelial origin.

Animals↗
PubMed1991

Protection of the reperfused heart by L-propionylcarnitine.

The effects of L-propionylcarnitine on mechanical function, creatine phosphate and ATP content, and lactate dehydrogenase leakage were studied in isolated perfused rat hearts exposed to global no-flow ischemia for 30 min followed by reperfusion for 20 min. Five and 10 mM L-propionylcarnitine resulted in a 100% recovery of left ventricular-developed pressure, whereas the recovery was only 40% in the hearts perfused without this agent. Ischemia-reperfusion caused a 85% loss of creatine phosphate and a 77% loss of ATP, which was prevented by 10 mM L-propionylcarnitine. Five millimolar L-propionylcarnitine protected the heart from the loss of creatine phosphate but not from the loss of ATP. Ten millimolar L-propionylcarnitine failed to improve the postischemic left ventricular-developed pressure, when it was added to the perfusate only after ischemia. L-propionylcarnitine alleviated the decrease of coronary flow in the reperfused hearts. Lactate dehydrogenase leakage was aggravated in the beginning of the reperfusion period by 10 mM L-propionylcarnitine. This adverse effect was, however, transient. L-Propionylcarnitine provides protection for the postischemic reperfused heart in a dose-dependent manner. The optimal time for administration is before the ischemic insult. High doses of this compound may perturb cell membrane integrity. Moreover, the present data point to an intracellular, metabolic, and perhaps anaplerotic mechanism of action of L-propionylcarnitine in cardiac ischemia-reperfusion injury.

Animals↗
PubMed1977

Capture myopathy in Elk in Alberta, Canada: a report of three cases.

Acute capture myopathy was diagnosed in 3 elk (Cervus canadensis). In 2 of the elk, hindlimb lameness developed within 10 days of capture. Clinical signs were not observed in the third elk, which was found dead 7 days after its capture. Elks 1 and 2 were euthanatized and all 3 animals were necropsied. In elks 1 and 2, rupture of the semi-membranosus muscle was associated with massive hemorrhage and swelling. Microscopic findings included hemorrhage, edema, myofibril fragmentation, and evidence of attempted muscular regeneration. In elk 3, several of the large muscles of the hindlimbs as well as the biceps brachii muscles of the forelimbs appeared pale, dry, and friable. Except for lack of hemorrhage and edema, microscopic findings in this elk were similar to those of elks 1 and 2.

Alberta↗
PubMed1977

Spectrum of immune response abnormalities in different clinical forms of tuberculosis.

In an attempt to explain the reasons for the development of different clinical forms of tuberculosis in different persons, their immunologic status was compared to their clinical patterns. The spectrum of immunologic abnormalities correlated with the clinical forms. Also, an inverse relationship between cell-mediated and humoral immune responses was observed. Immunologic abnormalities reverted to normal concomitant with clinical improvement on chemotherapy, suggesting that the abnormalities were the result of the illness rather than its cause. Malnutrition could have been the underlying factor for the immunologic deficiencies seen in some patients.

Antibodies, Bacterial↗
PubMed1977

Electron microscopy of rapid identification of animal viruses in hematoxylin-eosin sections.

Routine hematoxylin-eosin stained, paraffin sections were processed for electron microscopy, using a rapid method for localization of animal viruses. Formalin fixation was effective in preserving DNA as well as RNA viruses, however cellular fine structural details and organelles were not well preserved. The procedure is useful for morphological recognition of viral groups and as a rapid diagnostic aid for identifying viral disease.

Animals↗
PubMed1975

Microfibrillar structures in the nucleus and cytoplasm of amoeba proteus.

The presence of microfibrillar structures in the nucleus and cytoplasm of Amoeba proteus has been described after glutaraldehyde and osmium fixation. The possible roles of cytoplasmic microfibrils in the contraction process of amoeba and nuclear microfibrils in the formation of the honeycomb nuclear lamina are discussed.

Amoeba↗
PubMed1975

Amoeba proteus: the nuclear periphery.

This study extends previous work on the nuclear envelope and associated structures. It illustrates that the cylindrical structures of the honeycomb lattice are not attached to the nuclear envelope, although generally perpendicular and closely apposed to it, and that there is a complex arrangement of fibrillar material between the cylinders of the lattice. The relationship of nuclear helices to these structures is described and the possible mode of their transfer from nucleus to cytoplasm is discussed.

Amoeba↗
PubMed1975

Masking of pleomorphic glycogen sites by methanolic uranyl acetate.

Rat liver tissue was fixed in 2.5% glutaraldehyde buffered with cacodylic acid (pH 7.3) for 2 hr, washed twice in buffer, and postfixed in 2% osmium tetroxide at 4 C for 1 hr. The tissue then was dehydrated, infiltrated with and embedded in Epon by routine procedures. The ultrathin sections from this tissue, when stained with spectroscopic grade methanol saturated with uranyl acetate (SMUA) for 1 min followed by aqueous lead citrate (PbCi) (Reynolds 1963) for 5 min at room temperature, showed a uniform staining of all major cellular components except glycogen. The SMUA appeared to be specific for ribonuceloprotein granules, rendering them more prominent in the cytoplasm due to the lack of glycogen staining. The question of glycogen removal from the sections due to SMUA treatment was evulated using various extractions and staining methods. It appeared that SMUA pretreatment alters the subsequent binding ability of lead salts, resulting in lack of glycogen staining, although it does not remove the glycogen from the sections.

Acetates↗