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Biomedical subjects

R Bianchini

Publications and source records attributed to R Bianchini.

18 recordsLinked to original sources

Accreditation experience of radioisotope metrology laboratory of Argentina.

This work presents the experience developed by the Radioisotope Metrology Laboratory (LMR), of the Argentine National Atomic Energy Commission (CNEA), as result of the accreditation process of the Quality System by ISO 17025 Standard. Considering the LMR as a calibration laboratory, services of secondary activity determinations and calibration of activimeters used in Nuclear Medicine were accredited. A peer review of the (alpha/beta)-gamma coincidence system was also carried out. This work shows in detail the structure of the quality system, the results of the accrediting audit and gives the number of non-conformities detected and of observations made which have all been resolved.

Accreditation↗

[Sport activity in children and adolescents: temperament and emotional traits].

AIM: Aim of this study is to evaluate anxiety and temperament characteristics in developmental age subjects who practised agonistic sport (individual or team sports) in comparison with a sample group of subjects who practice no agonistic sports. METHODS: Sixty subjects aged from 10 to 16 years were enrolled in the study and divided into 3 groups: 20 subjects practised individual agonistic sport (Group A), 20 subjects practised team agonistic sports (Group B) and 20 subjects who practised non agonistic sport as control group, (Group C). The following tests were used: multidimensional anxiety scale for children (MASC) to evaluate anxiety, EAS scale (Buss e Plomin) to evaluate temperament (emotionability, activity, sociability and shyness for younger children). RESULTS: MASC scale scores underline generalized anxiety with higher significantly score in subjects who practised agonistic sports (Group A and B) compared with Group C. Anxiety symptoms were more evident in subjects who practised individual discipline compared with those who practised team sports. EAS scale indicated that temperament of subjects who practised agonistic sport was characterised by a considerable aptitude for sociability with low emotionability/activity levels in comparison to control group. CONCLUSION: None of the 3 groups showed a psychopathologic profile.

Adolescent↗

Neuropsychological assessment in children with absence epilepsy.

OBJECTIVE: To define cognitive deficits in children with absence epilepsy. BACKGROUND: Cognitive deficits have often been reported in children with epilepsy, but have rarely been characterized in patients with a specific epileptic syndrome. METHODS: Detailed neuropsychological testing was carried out on 16 right-handed children with absence epilepsy with similar clinical and EEG findings, and the findings were compared to 16 well-matched right-handed children without absence epilepsy. RESULTS: The authors found lower scores of measures of general cognitive functioning and visuospatial skills in patients with absence epilepsy, as compared to controls. Memory disturbances were also detected in absence epilepsy patients, with selective involvement of nonverbal memory and delayed recall. In contrast, verbal memory and language skills were relatively preserved. Patients whose seizures began at an earlier age seemed to have more severe cognitive deficits. CONCLUSION: Language skills tend to be relatively well preserved in children with generalized epilepsy, with more dysfunction seen in global terms rather than specific lateralizing deficits. Patients with absence epilepsy seem to show a similar neurocognitive profile that may be a reflection of the underlying epilepsy syndrome.

Adolescent↗

Developmental patterns and neuropsychological assessment in patients with carbohydrate-deficient glycoconjugate syndrome type IA (phosphomannomutase deficiency).

Carbohydrate-deficient glycoconjugate (CDG) syndrome type I due to phosphomannomutase deficiency (CDGIA) is the most common among a group of metabolic disorders characterized by a defective glycosylation of glycoconjugates. Clinically it is a multisystem disease with an important involvement of the central nervous system including pontocerebellar atrophy. Here the developmental patterns and results of neuropsychological assessment of four young adults with CDGIA syndrome are reported. The patients, aged 14-26 years, had classical clinical findings of CDGIA syndrome and olivopontocerebellar atrophy of severe degree. They had a marked delay in all areas of psychomotor development and gained to walk with aid, perform manipulative abilities and develop a communicative language after the 7th year. Later on, the acquired abilities remained stable, while self-help skills gradually improved, allowing the patients to join the family life. On neuropsychological assessment, there was mental retardation of variable degree with a special impairment of visuoperceptual skills, visuospatial organization, eye-hand coordination, verbal memory and language. Such findings, may be partially explained by the supratentorial atrophy in our patients and add more evidences to the role of the cerebellum and brainstem in the acquisition of non-motor cognitive functions. This study expands our understanding on the clinical spectrum of CDGIA syndrome and may be helpful for planning rehabilitation and education.

Adolescent↗

Combined coronary bypass grafting and mitral valve surgery: Early and late results.

It is reported that the mortality rate for combined coronary bypass grafting and mitral valve replacement is greater than for either isolated operation. To evaluate the effects of various predicting factors we analyzed the results of 67 consecutive patients undergoing combined mvr and CABG. The mean age was 61.3 +/- 7 years. There were 55 males and 12 females and the mean follow-up was 59.4 +/- 7 months. The hospital mortality rate was 13.4% (9/67). Preoperative NYHA functional class (p<0.05), left-ventricular motion score (increased scores indicating impaired function, (p<0.05), and aortic cross-clamp time (p<0.05) were associated with hospital mortality. There was no significant relationship of age (>60), cause of mitral valve disease, severity of mitral regurgitation, number of grafts, or previous myocardial infarction with hospital mortality. There were 7 late deaths, and survival at five years was 76.1%. Although there was a trend for preoperative NYHA class and aortic cross-clamp time to be associated with late survival, the only factor significantly related to late survival was global wall-motion score (p<0.05). Severity of mitral regurgitation and cause of mitral valve disease have been reported as being related to late survival, but we have found no such relationship. Our results indicate that both hospital and late mortality are strongly correlated with preoperative left ventricular function.

Adult↗

Influence of drug loading on coated beads release using air suspension technique.

Spherical granules were prepared by loading a drug such as Indobufen on 40-45 mesh non-pareil seeds using air suspension coating technique (Wurster process, Uniglatt). The drug was added by spraying a formulated aqueous dispersion onto inert granules in different amounts employing two drug particle sizes with different surface areas. Then all active beads were coated with different thicknesses of polymeric film using the same fluid bed employed for loading the drug. The coatings were applied from aqueous dispersions (pseudolatex) of ethyl cellulose (Aquacoat ECD-30). In this system the drug diffusion is governed by the intrinsic pore network of the membrane. The finest drug particle size gave the fastest drug release rates. Moreover, the lowest drug loadings on inert granules resulted in the slowest drug release. Nevertheless thicker film coats involved a delay of drug release. Further preparations were made to evaluate the influence of changing the seed size to 25-30 mesh, loading the same amount of the drug and obtaining the increased surface are of the individual bead. In order to improve the drug release profile, the effect of changing diethylphthalate to the more water-soluble triethylcitrate and the addition of hydroxypropylmethyl cellulose or polysorbate 80 were also evaluated. In this case the drug diffusion is controlled by dissolution of a part of membrane leaving small channels of polymer coating.

Chemical Phenomena↗

Oral controlled release optimization of pellets prepared by extrusion-spheronization processing.

Controlled release high dosage forms of a typical drug such as Indobufen were prepared as multiple-unit doses by employing extrusion-spheronization processing and subsequently film coating operations. The effects of drug particle size, drug/binder ratio, extruder screen size and preparation reproducibility on the physical properties of the spherical granules were evaluated. Controlled release optimization was obtained on the same granules by coating with polymeric membranes of different thickness consisting of water-soluble and insoluble substances. Film coating was applied from an organic solution using pan coating technique. The drug diffusion is allowed by dissolution of part of the membrane leaving small channels of the polymer coat. Further preparations were conducted to evaluate coatings applied from aqueous dispersion (pseudolatex) using air suspension coating technique. In this system the drug diffusion is governed by the intrinsic pore network of the membrane. The most promising preparations having the desired in vitro release, were metered into hard capsules to obtain the drug unit dosage. Accelerated stability tests were carried out to assess the influence of time and the other storage parameters on the drug release profile.

Cellulose↗

Fatal outcome in a metatypical, giant, "horrifying" basal cell carcinoma.

Mutilating, horrifying, aggressive basal cell carcinoma (BCC) is a rare cutaneous neoplasm. We report a case of mutilating BCC of the face and its peculiar histologic features. The primary lesion, before therapy, demonstrated typical BCC in association with lobules of metatypical cells. The recurrent BCC, after radiation therapy, had a metatypical pattern. We believe that the metatypical BCC is a potentially aggressive lesion. It is essential for this tumor to be recognized early and treated surgically.

Aged↗

The reaction of 3,4-epoxy-1-butene with deoxyguanosine and DNA in vitro: synthesis and characterization of the main adducts.

The reaction of guanosine with 3,4-epoxy-1-butene in acetic acid gives two main products of N-7 alkylation. After acidic hydrolysis the two aglycones have been isolated by h.p.l.c. and shown to be the regioisomeric 7-(2-hydroxy-3-buten-1-yl) guanine (I) and 7-(1-hydroxy-3-buten-2-yl) guanine (II), arising through nucleophilic attack by N-7 of the purine at the two oxirane carbons of 3,4-epoxy-1-butene. Spectral characteristics of both compounds are presented, including u.v., 1H-n.m.r. and mass spectra. Deoxyguanosine reacts with 3,4-epoxy-1-butene in 50% methanol-water at 37 degrees C to give the N-7 alkylated deoxynucleosides corresponding to I and II in a 59:41 ratio. The reaction rate depends on the nucleoside concentration, with second order rate constants at 37 degrees C of 1.6 X 10(-2) and 1.1 X 10(-2) h-1 M-1 for the formation of the two deoxynucleoside adduct corresponding to I and II, respectively. The same two compounds I and II in a similar (54:46) ratio have been identified after acidic or thermal hydrolysis of DNA which had been reacted with 3,4-epoxy-1-butene under similar conditions. The half life for the spontaneous depurination of I and II in the adducted DNA under physiological conditions (37 degrees C, pH 7.2) is 50 h.

Apurinic Acid↗

The effect of compression on the capillary microstructure of tablets.

The effect of compaction pressure on the capillary characteristics of compacts of a crystalline drug and of a pure polymer was studied by means of penetration and porosimetry measurements. Not only the constant but also the exponent m of the general penetration volume-time relationship V = Kmtm proved to be strongly dependent on the pressure: as the compaction pressure is increased, the value of m tends to decrease. The capillary penetration data also showed the development of a marked heterogeneity in the structure of the polymer compacts at the lower compaction pressures: at these pressures the surface layers showed a much lower penetrability than the inner core of the compacts, whereas at the higher pressures the structure of the compacts was homogeneous. Furthermore, on the basis of a generally used equation describing the powder consolidation process, it was possible to relate the most relevant variation of the kinetics of capillary penetration into the drug compacts to the deformation of the particles. It is concluded that the capillary penetration measurements can give useful information in the understanding of the behaviour of pharmaceutical powders under compression.

Permeability↗

[Our experience in microsurgery of rhino-sinus phlogosis].

Rhino-microscopy has made great progress in the diagnosis and surgical treatment of rhino-sinus phlogosis. In fact, a larger portion of the endonasal pathologies, which would not otherwise have been detected, can now be diagnosed through rhino-microscopic exploration. From the surgical point of view microscopy offers a clear, enlarged operating field. Moreover, with the aid of an autostatic speculum, it frees the surgeon to use both hands. These features make intricate, precise surgery possible thus enabling preservation of those structures which are not involved in the disease. At the same time it allows for re-establishment of the physiological functionality of the rhino-sinus cavity. Between 1980 and 1989 the microsurgery technique was employed in 950 cases of rhino-sinus cavity surgery. The results achieved using this technique were quite good and the number of complications was quite limited although the number of patients to be followed up over the years was not sufficient to draw any statistical conclusions. For several years now optic-fibre endoscopy has also been employed. This, too, is a functional technique although the method and instruments are totally different. It is not the intention of the authors here to assert that microsurgery is superior to endoscopy even though some technical details (i.e. the ability to operate with both hands) has made it easier to use, particularly for those accustomed to using a microscope. It is, however, felt that for rhino-surgery it is advisable to be familiar with both techniques so that they can be used alternately depending on the type of pathology and patient.

Chronic Disease↗

[Quality of life in patients undergoing mitral valve substitution].

AIM: The aim of the present study was to evaluate the quality of life after mitral valve replacement through the analysis of behavioural, psychological, functional, economic and working changes, as well as alterations in social and sexual life after surgery. SETTING: The study was performed before and after surgery in a cardiological ward. POPULATION: The population studied included 206 patients undergoing mitral valve replacement surgery. Results were compared with a control population of patients suffering from mitral valve disease and receiving medical therapy. METHOD: All patients were asked by medical staff to compile a series of five self-evaluation questionnaires: General Well-Being Schedule, Physical Symptoms Distress Index B, Social Participation, Sexual Satisfaction Unified Test and Work Performance and Satisfaction. RESULTS: Our results showed a significant improvement in the perception of quality of life after mitral valve replacement. In particular, the state of general well-being improved significantly with a clear reduction in symptoms. This was accompanied by a reduction in sexual activity and no change in social life or working capacity. Data obtained in the group of patients operated showed a significant improvement in the perception of the quality of life and psycho-physical well-being compared to patients receiving medical treatment alone. CONCLUSIONS: From this study it can be seen that patients undergoing mitral valve replacement surgery experience a marked improvement in the quality of life compared to pre-operative conditions and to the group of patients receiving medical therapy for mitral valve diseases. The use of specific working and social rehabilitation programmes can certainly optimise the results also with regard to affective relations.

Aged↗