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Biomedical subjects

R Biermé

Publications and source records attributed to R Biermé.

At least 19 recordsLinked to original sources
PubMed2001

[Short- and long-term benefits of smoking cessation].

The beneficial effects of withdrawal smoking on the disrupted physiological functions bound to tobacco spread differently: some weeks for smell, taste and breathing, to the less six months to recover tolerance to the effort without dyspnea. For the cardiac attack the risk bound to tobacco disappears after five years, and the risk for cancers after ten years. The expectation of life of the ex-smoker joined the one of non-smokers 15 years after the stop of smoking. The tobacco use by the women raise some particular problems. To convince the smoker to persevere in the decision of withdrawal smoking and to be free of dependences (pharmacological and psychological) it could be necessary to use the nicotinic substitutes and/or a psychological support. Stop smoking is the better and the most economic therapeutic to avoid recurrence of cardiac attack for a lot of authors.

Adolescent↗
PubMed1989

[Constitutional abnormalities of hemostasis: diagnostic strategy].

The most common clinical and biological expressions of constitutional abnormalities of haemostasis on which rests the diagnostic approach are influenced by the level where deficiency occurs as well as by the degree of this deficiency and its mode of transmission. Four situations encountered in practice are described: a severe form with loud and early clinical expression, a moderate form with subdued clinical expression but potential risk of haemorrhage, a genetic enquiry and the discovery of a biological abnormality. Concerning the first two situations, the stress is placed on their clinical signs, since peculiarities in a routine laboratory examination lead to a more appropriate exploration performed by a specialized laboratory.

Blood Coagulation Disorders↗
PubMed1988

Thin-layer agarose isoelectric focusing of alkaline phosphatase isoenzymes from human neutrophils.

An improved method for thin-layer agarose isoelectric focusing of alkaline phosphatases (AP) from human neutrophils is described. The solubilization of AP isoenzymes was studied with four detergents. The best results were obtained after sonication with Zwittergent 3-12 (1% final concentration), followed by butanol extraction and ultracentrifugation 105,000 x g for 1 h. The cytosol can be stored at 0 degrees C or -80 degrees C, but not at -20 degrees C. Dialysis of the cytosol against a Tris buffer, pH 7.5, was imperative prior to focusing for removal of the detergent. Enzyme visualization is enhanced by incorporation of ZnCl2 (3 mM) into the agarose gel. The focusing patterns consist of two sets of isoenzymes: two main zones with pI 6.4 and 6.8 and minor components with pI 4.2, 4.8 and 5.2.

Alkaline Phosphatase↗
PubMed1985

[Evaluation of the performance of an automatic coagulation tester: the Electra 700].

The Electra 700, an automatic coagulation tester recently available in France, was investigated for its performance on routine tests: Quick's test, APTT, thrombin clotting time test, assay of fibrinogen and prothrombin complex factors. Reproducibility was exceptionally good for Quick's test (CV less than 1%), excellent for the other tests (CV less than 3%), acceptability of the various commercial reagents very good, and standard linearity excellent. There was close correlation with manually obtained (electromagnetic B.M.) or semiautomated (KC 10) results (0.9948 to 0.9345). The apparatus is entirely automatic once specimens have been loaded, and it is particularly adapted to dealing with long series of specimens (60 samples). Its original loading system makes it possible to carry out two types of test concurrently, as well as to insert emergency tests. Economically, the cost of disposables and "reagent loss" is satisfactory; however, all tests are carried out relatively slowly, though this is compensated for by the fact that the reactions are completely automatic.

Blood Coagulation Tests↗
PubMed1983

[Monitoring anticoagulant treatment with the KEM-O-MAT 2HP autoanalyzer].

Synthetic chromogenic substrates were adapted to KEM-O-MAT 2HP for an automated monitoring of anticoagulant treatments, determination of factor X (S 2337) in oral anticoagulation, anti-Xa (Hepachrom) and anti-lla (S 2238) activities of heparin in prophylactic and curative heparin therapies. In all cases, these methods revealed high sensitivity, reproducibility, and a good correlation of their results with those of classical clotting assays. The present cost of synthetic substrates seriously limits the potential value of chromogenic assays for routine controls. However, the development of automated amidolytic techniques should provide an easier standardization in the laboratory control of anticoagulant treatments.

Anticoagulants↗
PubMed1983

[Evaluation of Owren's thrombotest in the monitoring of treatment with antivitamin K].

The need to standardize the monitoring of oral anticoagulant treatments has led some countries to adopt, generally, Owren's Thrombotest. Apart from needing only one reagent and being perfectly adapted to this type of monitoring, the thrombotest has the advantage of being able to be used on capillary blood and venous blood and of not being influenced by the storage of the blood samples. Its disadvantages are that it is slow to perform, difficult to automate and that it has a therapeutic range which is narrow and close to the limits of precision of the technique. The results of the Thrombotest correlate well with those of the Quick test, the activated cephalin time and the X level measured by the amidolytic technique. Until the chromogenic assays are developed, the Owren's Thrombotest provides a better homogeneity and standardization of the results coming from different laboratories.

Anticoagulants↗
PubMed1981

Evaluation of some platelet parameters in a group of elderly people.

Platelet production time (PPT), plasmatic beta-thromboglobulin (beta-TG), platelet density distribution, and mepacrine labelled granular content of the platelets were simultaneously determined in a group of elderly subjects. Vascular deterioration, assessed by the history and clinical examination, was variable in extension and severity. PPT was slightly but not significantly shortened, whereas the raised beta-TG level and diminished platelet density and granular content suggested an in vivo release reaction process. The results are not correlated with the apparent severity of vascular deterioration, and the release reaction may not be solely related to enhanced platelet-vessel wall interactions.

Adult↗