PubMed Health⌕ Search

Biomedical subjects

R Bonnichsen

Publications and source records attributed to R Bonnichsen.

At least 19 recordsLinked to original sources
PubMed1978

Preparation of crystalline bovine liver beta-glucuronidase.

A method is described for the preparation of pure beta-glucuronidase from bovine liver. The procedure includes ammonium sulfate, acetone and ethanol fractionation and a simple two-step ion exchange chromatography. The yield is acceptable and the method requires only standard laboratory equipment. The pure enzyme is easily crystallized from ammonium sulfate. Some practical applications of the pure beta-glucuronidase are discussed.

Animals↗
PubMed1975

Variations in human metabolism of methaqualone given in therapeutic doses and in overdose cases studied by gas chromatography-mass spectrometry.

The individual variations in human metabolism of methaqualone were studied after oral administration of therapeutic doses. The effect of antabuse on the drug metabolism as well as metabolites in some autopsy cases were also studied. The metabolites were identified using the GC-MS computer technique, and the relative amounts of the indicated metabolites were calculated from the sum of the peak heights in the gas chromatogram. The results show that the individual variations were small in the cases of therapeutic doses and that antabuse did not interact with the metabolism of methaqualone. Large amounts of one single metabolite 2-methyl-3-[phenyl(2'-methyl-4-hydroxy)]-4(3H)-quinazolinone was found in the bile from the two autopsy cases where the bile was also collected.

Administration, Oral↗