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Biomedical subjects

R Borota

Publications and source records attributed to R Borota.

At least 19 recordsLinked to original sources
PubMed1996

[Clinical use of hematopoietic growth factors--general principles].

Biology of haematopoietic growth factors in the process of haematopoiesis is well known, but their clinical utilization started with production of recombinant preparations. Today only preparations of Erythropoietin, GM-CSF and G-CSF are commercially at disposal. Absolute indications for utilization of haematopoietic growth factors are states caused by decreased production of certain classes of blood cells as a consequence of shortage of a growth factor necessary for production of a certain class. As these states are very rare, relative indications spread to other states characterized by a decreased number of blood cells or necessity for stimulation of haematopoiesis due to any other reason. This paper contains results of clinical researches only for those growth factors which are not commercially utilized.: M-CSF, Interleukin 3, PIXY321, SCF, Interleukin 6, Interleukin 11, Interleukin 1, Interleukin 2 and Thrombopoietin. Our institution utilizes only the preparations of Erythropoietin (Eprex) and G-CSF (Neuprogen) in 38 patients.

Hematopoiesis↗
PubMed1996

[Clinical use of erythropoietin].

Humoral regulation of erythropoiesis has been known for 100 years, while clinical utilization of recombinant human erythropoietin (rhEPO) only for two decades. It can be said that there is much experience in regard to indications, models and results of its clinical utilization. According to the standpoint of secretion of erythropoietin, anemias can be divided into those where secretion is increased and satisfactory, those where it is increased but not satisfactory and into those where it is not increased or it is even decreased. Anemias of the first group are not an indication for rhEPO utilization, the second group is relative and the third group absolute indication for its utilization. The best results are achieved with absolute indications and it is anemia in chronic renal insufficiency and nonphysiologic anemia of premature babies. Good results can be expected, but not predicted in relative indications, such as anemias in chronic infections, anemias in malignant diseases, myelodysplastic syndrome, aplastic anemia and other secondary anemias. Utilization of rhEPO is useful also in certain states without anemia, especially in transfusiology.

Anemia↗
PubMed1995

[The effect of blood from patients with idiopathic thrombocytopenia and thrombopoietin on the thrombocyte count in the blood of mice].

We examined effects of serum belonging to persons suffering from chronic autoimmune thrombocytopenia on the number of thrombocytes in the peripheral blood of mice. The serum, 0.2 ml, was given to groups of mice intravenously and then the number of thrombocytes was examined during 14 days. A significant decrease of thrombocytes was established in mice receivers during the period of 6 hours to 12 days (it was maximal from the 4th to the 6th day). The same serum was given to two groups of mice and then one of them was also given thrombopoietin preparation. The number of thrombocytes was established 4 and 6 days later and in the group where only serum was given there was a significant decrease of thrombocytes. In the group where thrombopoietin was given apart from the serum, the number of thrombocytes remained normal. On the basis of these findings it has been suggested that, using such an experimental model, a biological in vivo test could be made to demonstrate antibodies in autoimmune thrombocytopenias and to establish that thrombopoietin could be a useful drug in cases of chronic autoimmune thrombocytopenia.

Animals↗
PubMed1994

[Blood of patients with acute leukemia increases the leukocyte count in the peripheral blood of mice].

Serum of 23 patients suffering from acute myeloid leukemia were given to groups of six mice (s.c. 0.6 ml); 3 and 5 days later the total number of leucocytes, granulocytes and lymphocytes was determined in the peripheral blood of mice. Serums of all patients caused significant increase of leucocytes in mice 3 and/or 5 days after the serum's application. In most cases the increase was caused by increased number of granulocytes and lymphocytes, and in 6 cases only by increase of granulocytes or only lymphocytes. This increase can also be thought of as a stimulation of leukocytopoiesis by increased concentration of hematopoetic growth factors in blood of those suffering from acute leukemia.

Acute Disease↗
PubMed1993

Usage of quantitative static scintigraphy of the thyroid gland in defining the therapeutical dose of radioiodine.

In a group of 21 patients sent by endocrinologist for antihyperthyreosis therapy with radioactive iodine, we calculated and compared the weight of the thyroid gland by quantitative static scintigraphy, using two computed parameters--according to surface and volume of the gland. Statistically we came to the conclusion that weight of the thyroid gland calculated on the basis of surface gives significantly higher values than calculation by the use of volume. The calculation of weight of the thyroid gland using the surface of scan image is recommended, while quantitative static scintigraphy increases the standardisation of the procedure very much.

Adult↗
PubMed1991

[Quantitative static scintigraphy of the thyroid gland].

The paper describes a static scintigram computer processed by the Siemens-Euromeni method which gives rather accurate parameters concerning the overall functional thyroid tissue mass, as well as functions of a certain thyroid gland node. It facilitates the calculation of the dimensions of the right-angled field which borders the thyroid gland (cm), radioactivity uptake (% of a given dose) in the whole gland, left and right lobe, the ratio between the left and right lobe uptake, the surface (cm2) of the whole gland and of the left and right lobe, the ratio between the left and right lobe surfaces, the number of impulses (imp) registered in the whole gland and in the left and right lobe, the volume (cm3) of the whole gland and of the left and right lobe, the ratio between the left and right lobe volume, as well as the surface and radioactivity uptake (% of a given dose and % of the total uptake) in the marked nodes. An additional calculation could offer an uptake ratio per surface unit between the node and paranodal tissue which, by the use of suppression tests offers a very accurate assessment of the function and compensation of each node. The method represents a significant contribution to an accurate assessment of the functional mass as well as to the assessment of nodal changes of the thyroid gland.

Humans↗
PubMed1991

[The importance of intact parathyroid hormone determination in the evaluation of parathyroid gland function].

The study investigates the importance of determining the intact parathormone in the evaluation of parathyroid gland function in different conditions of change in their function and the alteration of parathormone metabolism: primary and secondary hyperparathyroidism, hypoparathyroidism and hyperthyroidism. The role and place of intact parathormone determination in routine diagnostic schemes of calcium homeostasis disorders was reviewed by the simultaneous determination of the indicators of calcium and bone metabolism and other calcium homeostasis hormone regulators, as well as the C- and N-terminal parathormone fragments and by establishing their mutual links with the values of the intact parathormone.

Humans↗
PubMed1990

[Radioimmunologic determination of growth factors. II. Comparative development of methods for the determination of hematopoietic growth factors].

On the basis of our long-term experience, presented are the current clinically useful methods for determining erythropoietin, the granulocytopoiesis growth factor and thrombocytopoietin with the results in their use. Facts are shown, concerning the long-used method of determining erythropoietin through the use of the biological test on polycythemic mice. In our conditions, mice treated with physiological solution (negative controls) have a Fe-59 incorporation below 0.5% of the given dose, while 0.2 units of the international erythropoietin standard (positive control) increases that incorporation to 3.0%. Also presented, are results of determining erythropoietin by means of the newest immunometric method which has shown exceptional features: the whole procedure, including necessary rinsings, lasts about six hours, and it is possible, in the course of one work day, to all at once precisely determine the erythropoietin concentration in 80 samples of human serum. This fact, as well as the outstanding sensitivity of the method which is about 2 mJ/ml (that is 50 times the sensitivity of the biological test) has as a result, the wide introduction of erythropoietin determination in the clinical use of today and the definition of its clinical importance. The measurement range of the method is from 2-200 J/L which can be extended further by specimen dilution. It is especially important that duplicate measurements at one point of the curve almost coincide, which indicates great method precision. The lowest measured concentration in our series amounted to 0 J/L and the greatest to greater than 250 J/L. Elevated values were found in 17 of the 114 serums.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1989

[Significance of highly sensitive (immunoradiometry assay) TSH in the evaluation of hypothalamo-hypophyseal-thyroid axis function].

A diagnostic value of immunoradiometric TSH assay in the evaluation of the function of hypothalamo-hypophyseal-thyroid axis was investigated in this paper. Normal values of basal TSH and TSH response during TRH test were determined by the use of ROC analysis. It was found out that there was a highly significant correlation between basal TSH and the responses during TRH test and that the diagnostic value of basal TSH in the evaluation of function of thyroid axis was almost identical to the diagnostic value of responses during TRH test. This finding pointed to the fact that basal TSH determination could replace the application of TRH test in diagnostic of thyroid axis disturbances. Comparing diagnostic value of basal TSH with other tests which served for the confirmation of thyroid axis disturbances, this test proved to be dominant, the most specific and the most sensitive in respect to other applied tests and could be regarded as the first step in diagnostic scheme of thyroid disturbances.

Humans↗
PubMed1989

[Radioimmunologic determination of growth factors. I. Determination of somatomedin C].

The authors' first experience in the determination of plasma somatomedin C concentration by the radioimmunologic method without extraction was put forward. The method itself was checked up through the routine indicators and the range of normal limits was established by the determination of this concentration in the plasma of 15 healthy persons. Values of plasma and serum determination greatly differentiated. By the determination of somatomedin C concentration and growth hormone during the insulin test in 8 persons it was shown that somatomedin C concentration did not change by the abrupt and short changes in growth hormone concentration. A good correlation was made by the comparison of sometomedin C and growth hormone basal concentration in 16 samples whereby the regulatory role of growth hormone in somatomedin C excretion was confirmed. In conclusion, radioimmunologic determination of sometomedin C in diagnostics and the therapy of growth disturbance was emphasized.

Female↗
PubMed1989

[Determination of 17-alpha-hydroxyprogesterone in the diagnosis of congenital adrenal hyperplasia].

On the basis of data from literature and by the use of commercial accessories radioimmunologic method of the determination od 17-alpha-hydroxiprogesteron (17-OHPG) serum level was determined in two variants: a) addition to the previous extraction, b) without extraction. It was initiated in the clinical work for diagnostic of congenital adrenal hyperplasia (CAH) induced by the enzymatic block of C21-hydroxilasis. 17-OHPG level was determined in a group of 15 children aged 2-12 of both sexes not suffering from endocrinous diseases. Mean values od X = 3.2 +/- 2.7 nmol/L and X = 4.3 +/- 3.3 nmol/L were obtained under a) and b), respectively. Also, 17-OHPG concentration was determined in 15 women without any endocrinous diseases by the method under b) and mean value of X = 2.5 +/- 1.7 nmol/L was obtained. 17-OHPG level was determined in a group of 7 children in whom CAH had been verified 3 days after the interruption of the substitutional therapy. According to the results of the investigation it was pointed out that there was a marked increase in 17-OHPG serum level (particularly by the use of b) method) which was far outside the range of reference values. In cases both with 17-OHPG determination and the daily pregnantriol excretion it was noticed that the increase in 17-OHPG was almost always accompanied by the increase in pregnantriol as well. Cortisolemia ranged from normal up to very lowered values.(ABSTRACT TRUNCATED AT 250 WORDS)

17-alpha-Hydroxyprogesterone↗
PubMed1988

The effect of radiopharmaceutical choice on the assessment of the relative renal function in upper urinary tract obstruction.

The relative function of the obstructed kidney (RFOK) was assessed in 43 adult patients with upper urinary tract obstruction (UUTO) using 99mTc-DTPA and 131I-ortho-iodohippurate (OIH) dynamic studies and 99mTc-DMSA dynamic and static studies. The patients were divided into five groups according to the duration and degree of obstruction. Findings were as follows: a) in patients with the first occurrence of acute severe obstruction (group 1), the relative glomerular filtration rate (GFR) was significantly less than the relative effective renal plasma flow (ERPF); b) in patients with chronic severe obstruction and long term uroinfection, the relative ERPF decreased significantly compared with the relative GFR; c) the RFOK calculated from the DMSA dynamic study was the same as both the relative GFR or ERPF in any group; d) the RFOK calculated from the DMSA static study seemed to parallel the relative ERPF more closely than the relative GFR, but in group one it was significantly higher than any of three other estimates. It is concluded that 99mTc-DTPA is the radiopharmaceutical of choice in obstructive uronephropathy, but when interpreting the RFOK, the time course and severity of obstruction, the presence or absence of uroinfection should also be considered.

Adult↗