The origin of pain in 'ischemic-diabetic' third-nerve palsy.
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Animals↗
Biomedical subjects
R Bortolami
Publications and source records attributed to R Bortolami.
Orbital pain and unruptured carotid-posterior communicating artery aneurysms: the role of sensory fibers of the third cranial nerve.
Intact aneurysms of the carotid siphon at the point of take-off of the posterior communicating artery may exhibit orbital pain, whether associated with oculomotor palsy or not as a warning symptom prior to rupture. In order to explain this symptom the hypothesis of a sensory pathway within the third cranial nerve, which is liable to compression by the enlarging aneurysm sac, has been investigated. Data from human autopsy material show evidence of sensory ganglion cells within the rootlets of the oculomotor nerve; furthermore, studies in animals prove that the third nerve contains sensory fibers which run proximally along the nerve bundles, enter the brainstem and reach the spinal trigeminal nucleus. These fibers come from the ophthalmic division of the fifth nerve and join the third nerve at the level of the lateral wall of the cavernous sinus. Although a number of questions remain to be solved, the presence of a sensory pattern within the third nerve could account for fronto-orbital pain from enlarging aneurysms impinging on the third nerve itself.
Animals↗
Localization of motoneurons innervating the extraocular muscles of the sheep by retrograde fluorescent tracers.
Retrograde transport of the fluorescent tracers Fast blue, Evans blue, Diamidino yellow dihydrochloride, and Propidium iodide was used to determine the location of the motoneurons innervating the extraocular muscles of the sheep. An extensive superposition among the motor pools of the oculomotor nucleus (ON) has been observed. In the rostral third of the ON, a considerable merging occurs between obliquus ventralis and rectus medialis motoneurons and also between rectus ventralis and rectus medialis motoneurons. In the middle third of the ON, rectus dorsalis and levator palpebrae superioris motoneurons are intermingled with each other, and also with obliquus ventralis motoneurons dorsally and with rectus medialis motoneurons ventrally. The rostral portion of the trochlear nucleus overlaps with the caudal pole of the ON. The motoneurons innervating the obliquus dorsalis muscle are mainly contralateral with few ipsilateral exceptions. The retractor bulbi muscle receive the innervation by both the abducens and accessory abducens nuclei.
Abducens Nerve↗
High incidence of multiple-bag fiber muscle spindles in the articularis humeri muscle of the horse.
The articularis humeri (AH) muscle of the horse is a small muscle composed of histochemically identified type I and IIA extrafusal fibers and a large number of muscle spindles. A total of 150 complete spindles with both spindle poles available were examined in serial transverse sections. On the basis of myosin ATPase-staining reactions after alkaline and acid preincubations, four types of intrafusal fibers, namely, bag1, bag2, "mixed" bag, and chain fibers, were identified. A high proportion of the spindle population (62.6%) consisted of multiple-bag spindles containing three or more (up to six) bag fibers. Also one-bag-fiber spindles were observed. The one-bag-fiber spindles containing a bag2 fiber could be traced into tandem linkages. "Mixed" bag intrafusal fibers, differing in their ATPase staining profile at the two poles, were found in spindles containing also at least one bag1 and one bag2 fiber. An unusually long extracapsular tract (up to 5,500 microns) of the bag intrafusal fibers was observed.
Animals↗
Peripheral territory and neuropeptides of the trigeminal ganglion neurons centrally projecting through the oculomotor nerve demonstrated by fluorescent retrograde double-labeling combined with immunocytochemistry.
The peripheral territories of sheep trigeminal neurons which send their central process to the brainstem through the oculomotor nerve were investigated by the use of fluorescent tracers in double-labeling experiments. For this purpose Diamidino yellow (DY) injection into the oculomotor nerve was combined with Fast blue (FB) injection either into the extraocular muscles (EOMs), or the cornea, or the superior eyelid. Double-labeled DY + FB cells were found in the ophthalmic region of the trigeminal ganglion in addition to single-labeled DY or FB cells. The DY and DY + FB-labeled trigeminal cells were analysed immunocytochemically for their content of substance P (SP)-, calcitonin gene-related peptide (CGRP)-, and cholecystokinin-8 (CCK-8)-like. All single-labeled DY cells showed SP-, CGRP- or CCK-8-like immunoreactivity. Double-labeled DY + FB neurons innervating the EOMs were immunoreactive for each of the three peptides, whereas double-labeled neurons supplying the cornea were only CGRP-like positive. The findings suggest that, in the sheep, trigeminal neurons which send their process centrally through the oculomotor nerve supply the EOMs, the cornea, and the superior eyelid and contain neuropeptides which are usually associated with pain sensation.
Animals↗
The morphology and location of atrial specific granules and the demonstration of atrial natriuretic factor in porcine, lapine and bovine heart by immunoelectronmicroscopy.
The atrial specific granules (ASGs) were studied in samples collected from the right and left auricles of conventionally slaughtered cows (10), pigs (16) and rabbits (8). In addition, the presence of atrial natriuretic factor (ANF) was detected by immunocytochemistry. Mature ASGs, characterized by the presence of highly osmiophilic and electron-dense material surrounded by a membrane, were present in all atrial myoendocrine cells and their diameters ranged from 100 to 470 nm in pigs, from 100 to 235 nm in cattle, and from 125 to 275 nm in rabbits. Immunoelectronmicroscopical studies revealed the presence of ANF in the ASGs of pigs and cattle, whereas anti-ANF polyclonal serum failed to detect any significative reaction in lapine ASGs. The ultrastructural features of the ASGs of pigs, cattle and rabbits described may be useful in comparing the morphological picture of several cardiac endocrine pathological conditions.
Animals↗
Analysis of the sternotrachealis muscle fibers in some Anseriformes: histochemistry and sex differences.
Histochemical characteristics and sizes of the fibers of the sternotrachealis (ST) muscle have been investigated in some Anseriformes (mallard, Pekin duck, Muscovy duck, and goose) of both sexes. A sexual dimorphism has been shown in the muscle of the species examined. In the mallard and Pekin duck, the male ST muscle shows type IIIA fibers in addition to the type I, IIA, and IIB fibers observed also in the female. In the Muscovy duck, the male muscle has only type I and IIA fibers, whereas the female muscle presents type I fibers and both types IIA and IIB fibers. Moreover, the mean frequencies for each fiber type were significantly different between males and females. In the goose, both male and female muscles present only type I and IIA fibers. In all the species examined, the mean areas of each fiber type are significantly different between male and female, being always larger in the male muscles. The anatomical sexual dimorphism observed in the ST muscle is discussed in relation to function.
Adenosine Triphosphatases↗
An immunohistochemical approach to the intrafusal fibers of extraocular muscle spindles in sheep, cow, and pig.
Intrafusal muscle fibers of the extraocular muscles (EOMs) of the sheep, cow, and pig were studied histochemically and immunohistochemically. In sheep and cow spindles, three intrafusal fiber types, namely the bag1, bag2, and chain fibers, were identified by a combination of standard histochemical methods and immunohistochemical staining with antibodies selective for slow-tonic (antitonic ALD) and slow twitch (anti-I BA-D5) myosin. The bag1 and bag2 fibers appeared immunologically different on the basis of their differential reactivity with the two antisera. Anti-tonic ALD preferentially stained the bag1 fibers, whereas anti-I BA-D5 labeled the bag2 fibers. Chain fibers did not react with either antisera. In the pig EOM spindles, in general, one bag and some chain intrafusal fibers were identified. The bag fiber was labeled by anti-tonic ALD, but it did not react with the anti-I BA-D5. These findings point to the existence in pig EOM spindles of only one bag fiber antigenically similar to the bag1 fiber of the other species examined.
Adenosine Triphosphatases↗
Simultaneous cell death in the trigeminal ganglion and in ganglion neurons present in the oculomotor nerve of the bovine fetus.
A well-developed ganglion and scattered ganglion cells are present in the intracranial portion of the oculomotor nerve during the first half of fetal life in the ox. In the second half of fetal life a dramatic reduction of the ganglion cells associated with the oculomotor nerve occurs because of spontaneous cell death. Concomitantly, the same phenomenon of cell death is found in the trigeminal ganglion, especially in its rostromedial portion. Free degenerating perikarya can be found in the cavernous sinus.
Animals↗
Different intrafusal fiber composition of spindles in sheep and pig extraocular muscles.
Histochemical profiles of intrafusal fibers have been examined in muscle spindles of extraocular muscles of sheep and pig. Results show that in the sheep the intrafusal content presents, in addition to chain fibers, at least one bag1 and one bag2 fiber, whereas in the pig almost all the spindles are one-bag-fiber [corrected] spindles.
Adenosine Triphosphatases↗
On the nature of the afferent fibers of oculomotor nerve.
The oculogyric nerves contain afferent fibers originating from the ophthalmic territory, the somata of which are located in the ipsilateral semilunar ganglion. These primary sensory neurons project to the Subnucleus Gelatinosus of the Nucleus Caudalis Trigemini, where they make presynaptic contact with the central endings of the primary trigeminal afferents running in the fifth cranial nerve. After complete section of the trigeminal root, the antidromic volleys elicited in the trunk of the third cranial nerve by stimulating SG of NCT consisted of two waves belonging to the A delta and C groups. The area of both components of the antidromic volleys decreased both after bradykinin and hystamine injection into the corresponding cutaneous region and after thermic stimulation of the ipsilateral trigeminal ophthalmic territory. The reduction of such potentials can be explained in terms of collision between the antidromic volleys and those elicited orthodromically by chemical and thermic stimulation. Also, capsaicin applied on the nerve induced an immediate increase, followed by a long lasting decrease, of orthodromic evoked response area. These findings bring further support to the nociceptive nature of the afferent fibers running into the oculomotor nerve.
Animals↗
The fine structure of the area postrema of the sheep.
The ultrastructural features of the area postrema (AP) were investigated in the suckling lamb, weaned lamb and adult sheep. No morphological differences were observed between lambs and sheep. Unciliated ependymal cells, linked by zonulae adherentes-type junctions and gap junctions, cover the AP ventricular surface. Clusters of pyriform neurons, glial cells, and axons are present in the parenchyma. The blood vessels are surrounded by wide perivascular spaces, which present an inner and outer basal lamina. The capillaries are of the fenestrated type. Perivascular glial cells rest on the outer basal lamina of the perivascular space and form a continuous ensheathment with their cell bodies or with flattened interdigitating processes. Along adjacent perivascular glial processes gap junctions are present. From our ultrastructural observations it appears that the overall cellular morphology of AP of the sheep does not differ substantially from that of monogastric mammals.
Aging↗
Fiber sizes and histochemical characteristics of the rectus abdominis muscle of the rabbit under conditions of pregnancy and mechanically induced stress.
Histochemical properties and muscle fiber diameters of the rectus abdominis (RA) muscle of the female rabbit were compared with those of RA muscle of (1) pregnant females at term of pregnancy (30 days); (2) pregnant females in which the stretch on the abdominal wall natural to pregnancy was artificially prolonged with a 40-day period of intraabdominal appliance; (3) virgin females subjected to intraabdominal appliance for 30, 45, 60, and 70 days. The RA muscle of the control female rabbits is composed of 29.73% type I, 12.13% type IIA, 57.59% type IIB, and 0.54% type IIC fibers. The stimulation on RA muscle due to either normal pregnancy or implant in virgin females provoked changes in muscle fiber diameters only; with the normal pregnancy plus the subsequent period of mechanical stimulation, changes occurred not only in muscle fiber diameters, but there was also a significant increase in the percentage of type I fibers and a concomitant decrease of type IIA and IIB fibers. The fiber-type pattern in the RA muscle of male and female rabbit also was compared. The RA muscle of the male rabbit showed more type IIA and less type IIB fibers than found in that of the female. Type I fibers were larger in the female, but type IIA fibers were larger in the male. In addition, male rabbits also were subjected to 30, 45, 60, and 70 days of RA muscle stimulation by means of an intraabdominal appliance to ascertain if the influence of such stimulation upon the RA muscle differed between the two sexes. Some differences in the response of the male RA muscle were noted.
Abdominal Muscles↗
Histochemistry of intrafusal fibers in extraocular muscles of the sheep and pig.
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Animals↗
Sex differences in the histochemical characteristics of the sternotrachealis muscle of the duck.
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Animals↗
[Determination of the peripheral field of several trigeminal afferent fibers present in the oculomotor nerve].
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Afferent Pathways↗
Histochemical types and sizes of fibers in the rectus abdominis muscle of guinea pig: adaptive response to pregnancy.
Effects of pregnancy stimulation upon histochemically assessed myofibrillar ATPase and muscle fiber diameters were analysed in the rectus abdominis (RA) muscle of guinea pig. Samples of the muscle were taken at 30, 40, 50, 60, and 70 days of pregnancy and compared with samples of the same muscle taken from nonpregnant guinea pigs. Changes in muscle fiber proportions were noted through the course of pregnancy. Starting from 50 days of gestation an increase in type I fibers and a decrease in type IIB fibers were noted. Increase in muscle fiber diameters was also observed in type I, IIA, and IIB fibers. In addition, the RA muscle of the male guinea pig was compared with that of the female guinea pig and showed more type IIA and less type IIB fibers and all the three fiber types were larger than those of the female.
Abdominal Muscles↗
Influence of oculomotor nerve afferents on central endings of primary trigeminal fibers.
Painful fibers running in the third nerve and originating from the ophthalmic trigeminal area send their central projections at level of substantia gelatinosa of nucleus caudalis trigemini. The central endings of these fibers form axoaxonic synapses with trigeminal fibers entering the brain stem through the trigeminal root. The effect of electrical stimulation of the third nerve central stump on the central endings of trigeminal afferent fibers consists in an increased excitability, possibly resulting in a presynaptic inhibition. This inhibitory influence is due to both direct and indirect connections of the third nerve afferent fibers with the trigeminal ones.
Action Potentials↗