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R Botella

Publications and source records attributed to R Botella.

At least 37 records · Page 2Linked to original sources
PubMed1998

Mucinous metaplasia of apocrine duct.

Mucinous syringometaplasia is a rare condition characterized by the presence of mucinous cells lining eccrine ducts. Because most occurrences are on the palms and soles, the disorder is generally considered to be the result of chronic trauma or pressure. We describe an example of mucinous metaplasia involving an apocrine duct on the left arm of a 57-year-old woman. Histopathologic study demonstrated that in deeper areas of the invagination, the lining of the lumen consisted of columnar cells showing decapitation secretion and that the invagination was connected to a rudimentary hair follicle. These histopathologic features support the possibility that the involved duct was an apocrine one. We discuss the differential diagnosis with those cutaneous adnexal neoplasms in which the presence of mucinous cells has been described.

Adenoma, Sweat Gland↗
PubMed1998

Inhibition of murine melanoma growth by granulocyte-macrophage colony stimulating factor gene transfection is not haplotype specific.

Granulocyte-macrophage colony stimulating factor (GM-CSF) has been shown to inhibit the growth and progression of murine melanoma cells in syngeneic C57BL/6 (H-2b) recipient animals. We now demonstrate that this effect is not specific to melanomas derived from a single strain of mice by examining the subcutaneous growth of K1735 murine melanoma cells (H-2k) transfected with GM-CSF in a syngeneic mouse model. Non-GM-CSF-secreting melanoma cells (parental K1735 and K1735 cells transfected with the GM-CSF gene in antisense orientation) generated tumours that reached a mean volume of 4000 mm3 30-40 days, with a mean survival of 40 days after tumour cell injection. In contrast, 90% of the mice injected with three different clones of GM-CSF-producing K1735 melanomas developed no measurable tumours and were healthy and tumour-free when followed for over 300 days post-inoculation. Additionally, mice injected with GM-CSF-secreting K1735 cells developed long-lasting immunity to the parental melanoma cell line challenge in vivo. A dense neutrophilic and lymphocytic inflammatory infiltrate as well as large numbers of dendritic cells were detected only at the inoculation sites of the GM-CSF-producing melanoma cells. Thus, these studies demonstrate for the first time that GM-CSF inhibits melanoma growth in a second genetically distinct MHC tumour-host model system and further support the use of GM-CSF in clinical trials in the treatment of advanced malignant melanoma in humans.

Animals↗
PubMed1998

Segmental arrangement of multiple, partly congenital and partly acquired melanocytic nevi.

A 15 year-old girl presented with numerous congenital melanocytic nevi, occasionally hairy, with a segmental distribution at the left pre-auricular region. On the left side of the back of the neck there were multiple melanocytic nevi with a warty appearance, which had started to appear when she was 5 and which had remained stable from the time she was 10. These lesions had a distribution reminiscent of an epidermal nevus with a pattern similar to Blaschko's lines. On general physical examination there was a moderate degree of idiopathic scoliosis, with a left lumbar curvature. Biopsies were taken from both types of nevus which were diagnosed as congenital compound melanocytic nevus and acquired compound melanocytic nevus respectively. We consider that the segmental distribution of the melanocytic nevi of this patient supports the theory that a genetic defect determined the appearance of both these congenital and acquired lesions.

Adolescent↗
PubMed1996

Antitumor effects of granulocyte-macrophage colony-stimulating factor production by melanoma cells.

The use of immunomodulating gene therapy in the treatment of malignant disease is under intensive investigation. In this study, we examined the potential of melanoma-derived granulocyte-macrophage colony-stimulating factor (GM-CSF) to inhibit melanoma progression in a murine model. The HGH18 murine melanoma cell line was transfected with the murine GM-CSF gene in a SV40 expression vector that resulted in melanoma clones that produced varying amounts of GM-CSF. Syngeneic mice inoculated s.c. with HFH18 parental melanoma cells or HFH18 cells transfected with the GM-CSF gene n the noncoding 3'-5' orientation [HFH18/GM-CSF(-) cells] develop large tumors that reach a mean tumor volume of 3300 mm3 by day 30. In contrast, animals inoculated with two melanoma clones producing high levels of GM-CSF [HFH18/GM-CSF(++) and HFH18/GM-CSF(+ + +)] either completely reject the tumor cells or develop tumors with a mean volume of only 40 mm3. In comparison, animals inoculated with a melanoma clone producing low levels of GM-CSF [HFH18/GM-CSF(+)] develop large tumors averaging 2000 mm3, thus demonstrating a dose-response effect of tumor inhibition by melanoma-derived GM-CSF. Additionally, vaccination with irradiated GM-CSF-producing melanoma cells conferred optimal immunogenicity against a subsequent challenge with HFH18 cells. Tissue sections from excised GM-CSF-producing tumor cell inoculation sites but not from HFH18 parental or HFH18/GM-CSF(-) inoculation sites demonstrate a dense inflammatory infiltrate composed of neutrophils, tissue macrophages, and numerous CD4- and CD8-positive lymphocytes but few melanoma cells. Large numbers of dendritic cells and cells expressing the B7-2 costimulatory molecule are detected only within HFH18/GM-CSF(+ + +) melanoma inoculation sites. Our results lend further support to clinical trials of GM-CSF gene therapy in the treatment of advanced malignant melanoma, possibly by the recruitment of dendritic antigen-presenting cells.

Animals↗
PubMed1996

Livedo reticularis and thrombotic purpura related to the use of diphenhydramine associated with pyrithyldione.

We describe the case of a young woman who habitually took large doses of a combination of diphenhydramine and pyrithyldione. She complained of arthralgia, painful recurring plaques and nodules, together with persistent reticular purpuric mottling and areas of necrosis on her legs. There were positive antinuclear antibodies and rheumatoid factor and histologically massive thrombosis of the dermal vessels without signs of vasculitis. Other cases of similar adverse reactions attributed to this drug combination used as a hypnotic have been described.

Adult↗
PubMed1995

Necrolytic migratory erythema without glucagonoma in patients with liver disease.

BACKGROUND: Necrolytic migratory erythema (NME) is an uncommon inflammatory dermatosis with a distinct clinical and histologic appearance. NME is usually associated with glucagonoma. Only a few cases of NME in the absence of glucagonoma have been previously reported. OBJECTIVE: We sought to understand further the pathogenesis of NME by analyzing data from three patients. METHODS: Three patients were examined both clinically and histopathologically. RESULTS: Each patient had an extensive erythematous scaling eruption in intertriginous, perioral, and acral areas, and a markedly red, smooth tongue. Skin biopsy specimens showed confluent parakeratosis, epidermal pallor, papillary edema, and a lymphohistiocytic infiltrate. Two patients had alcoholic liver disease and one had liver dysfunction as a result of hemochromatosis. Serum albumin level was depressed, and liver enzyme values were increased in all three patients. Glucagonoma was undetectable in these patients. CONCLUSION: In the absence of glucagonoma, hepatocellular dysfunction and hypoalbuminemia appear to be the most common factors associated with NME.

Adult↗
PubMed1995

Tricoadenoma of Nikolowski.

The patient, aged 50 years, with no relevant clinical history, complained of a symptomless, soft tumor of the right buttock that had been present for many years. On examination, it was found to be pediculate, oval, somewhat erythematous, firm on palpation, and with a central keratin plug. The greatest diameter of the lesion was 1.2 cm (Fig. 1). On surgical removal of the lesion, it was seen to be composed of a symmetric intradermal proliferation of cystic formations, uniformly distributed throughout and made up of flat, pluristratified epithelium with laminated keratin. There was little difference in the thickness of the walls of the cysts (Fig. 2). Some cysts were attached to a short tadpole-shaped, epithelial cord and surrounded by a scanty fibroblastic stroma. No hairmatrix-like differentiation nor basaloid structures were seen, nor were atypical cells present. There was hardly any inflammatory infiltration.

Buttocks↗
PubMed1994

Contact dermatitis in children.

272 children up to the age of 14 years were patch tested during a 10-year period (1982-1991). 101 children (37.1%) gave positive reactions to 1 or more allergens. Of these, 54.4% (57/101), were considered relevant. The main allergens were, in order of frequency: nickel, rubber compounds, mercuric chloride, cobalt salts, thimerosal, benzoyl peroxide and fragrance mix.

Adolescent↗
PubMed1993

Cutaneous lymphadenoma: a case report and review of the literature.

We report a case of cutaneous lymphadenoma, a tumour first described by Santa Cruz and Barr under the name of 'lymphoepithelial tumour'. Twenty-three cases have been reported to date. The commonest site is the head and neck, and it is most commonly diagnosed in the fourth and fifth decades. The usual clinical diagnosis is basal cell carcinoma. To date, no recurrences have been observed after local excision. The tumour is composed of dermal lobules with a biphasic pattern of epithelial and lymphoid cells. It has been suggested that it arises as a result of faulty interaction between lymphocytes and epithelial cells.

Adult↗