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Biomedical subjects

R Bowers

Publications and source records attributed to R Bowers.

17 recordsLinked to original sources
PubMed1990

Kinetics of HIV-1 replication and intracellular accumulation of particles in HTLV-I-transformed cells.

The replication kinetics of HIV were examined in HTLV-I-transformed MT-2 cells. The duration of the initial replication cycle was 20 hours, determined by the first detection of infectious progeny virus, development of syncytia, and production of viral RNA and protein. A phase of exponential virus production followed until 62 h postinfection. Cell death occurred in the final phase of infection during which infectious virus production remained constant even though viral RNA and protein production increased at an exponential rate. Accumulations of HIV particles were observed within cytoplasmic vacuoles of infected MT-2 cells. Although cell lysates contained high titers of infectious virus, our data show that an increasing proportion of particles produced late in infection were not infectious.

Cell Line, Transformed↗
PubMed1979

Increased sheep lung vascular permeability caused by Escherichia coli endotoxin.

We infused Escherichia coli endotoxin, 0.07-1.33 microgram/kg, intravenously into chronically instrumented unanesthetized sheep and measured pulmonary arterial and left atrial pressures, lung lymph flow, lymph and blood plasma protein concentrations, and arterial blood gases. Endotoxin caused a biphasic reaction: an early phase of pulmonary hypertension and a long late phase of steady state increased pulmonary vascular permeability during which pulmonary arterial and left atrial pressures were not increased significantly and lung lymph flow was 5 times the baseline value. Lymph: plasma total protein concentration ratio during the late phase (0.76 +/- 0.04) was significantly (P less than 0.05) higher than during baseline (0.66 +/- 0.03). The lymph response was reproducible. Lung lymph clearance of endogenous proteins with molecular radii (r) 35.5 to 96 A was increased during the steady state late phase of the reaction, but, as during baseline, clearance decreased as r increased. The endotoxin reaction was similar to the reaction to infusing whole Pseudomonas bacteria, except that endotoxin had less effect on pressures during the steady state response and caused a relatively larger increase in lymph clearance of large proteins. We conclude that E. coli endotoxin in sheep causes a long period of increased lung vascular permeability and may have a greater effect on large solute pathways across microvessels than do Pseudomonas bacteria.

Animals↗
PubMed1977

Blood viscosity, red-cell flexibility, haematocrit, and plasma-fibrinogen in patients with angina.

Whole-blood viscosity, haematocrit and plasma-fibrinogen concentration were measured in 22 patients with angina and 22 controls. All four variables were significantly higher in patients with angina. When the viscosity was corrected to a standard haematocrit (45%), however, there was no significant difference in the mean viscosity of the two groups, indicating that the higher viscosity in patients with angina is the result of the higher haematocrit. Increased red-cell flexibility tends to counteract the effect of increased plasma-fibrinogen concentration, which tends to increase blood-viscosity.

Adult↗
PubMed1977

Deep venous thrombosis in patients having aorto-iliac reconstruction.

The 125I-labelled fibrinogen test has been used to assess the incidence of deep venous thrombosis in 88 patients who had undergone elective aorto-iliac reconstruction. Deep venous thrombosis was detected in 18 (20.5 per cent). The results suggest that these patients merit prophylaxis.

Adult↗