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Biomedical subjects

R Brassard

Publications and source records attributed to R Brassard.

At least 19 recordsLinked to original sources

Comparison of dietary fiber contents of selected baby foods from two major brands in Canada using three methods.

Of the two major brands of baby foods in Canada, one reports lower dietary fiber values than the other, although the products appear to be similar. To investigate the reasons for this discrepancy, seven selected samples of baby foods from both brands were analyzed for total dietary fiber (TDF) according to the Mongeau (rapid Health Protection Branch; HPB) method. Two cereals were also analyzed by using the Prosky and the Englyst (nonstarch polysaccharide; NSP) methods as an internal check on the methodology as well as a means of investigating the reasons for the discrepancies. The sampling included at least four different lots of each product (cereals, fruits, vegetables, and legumes). Each lot was analyzed individually. The TDF values determined using the rapid HPB method were in agreement with those obtained by other dietary fiber methods. Comparison between manufacturer-reported and measured values showed that the low values reported in brand A products were due, in part, to under-reporting of TDF content: measured TDF values were significantly higher than manufacturer-reported values. For brand B products, the manufacturer-reported and measured TDF values were in general agreement. This shows that a large part of the discrepancy between the two brands was due to methodological problems associated with measuring TDF in brand A. Differences in TDF content were also apparent as shown by the fact that brand A TDF values were consistently lower than those of brand B when both were measured by the same method. The differences in TDF content were not explained by differences in the polysaccharide composition of the fiber residues or by differences in water content. Although the limited number of samples does not allow any general conclusion about the TDF content of specific brands, the results show that formulation and/or manufacturing differences may influence TDF values in processed baby foods.

Canada↗

Ureteropelvic urinary extravasation due to iliac artery aneurysm.

We report a case of left-sided hydronephrosis and ureteropelvic urinary extravasation due to a large left iliac artery aneurysm. Urinoma was diagnosed preoperatively by contrast-enhanced computed tomography. The patient was successfully treated by percutaneous nephrostomy and ureteral double J stent placement followed by staged operative repair.

Aged↗

Effects of amylose and wheat bran on the levels of blood serum urea nitrogen (BUN), other blood parameters, growth and fecal characteristics in rats.

A basal diet (containing 20% soybean protein isolate) was supplemented with limiting amino acids (AA, methionine, threonine and tryptophan); wheat bran (WB, 24.3%); amylose (AM, 10%) or a combination of AA and WB or AM to investigate their effects on the levels of blood serum urea nitrogen (BUN), other blood parameters, growth and fecal characteristics in young rats. After 3 weeks feeding, supplementation of the basal diet with AA or WB resulted in improved growth (gain/food ratio) while the addition of AM had no effect on rat growth. Addition of WB to the basal diet had no effect on BUN while the addition of AA and AM caused about 20 and 12% reduction in BUN values, respectively. Rats fed the AA-AM diet had the lowest BUN values (42% lower than those fed the basal diet). Fresh volume, fresh weight, moisture and dry weight of feces in rats fed the WB diets were about four to five fold higher than in those fed the AM diets. The results suggested that BUN may not be a good predictor of growth and protein quality in diets containing AM or a source of dietary fiber (WB).

Amylose↗

Enhanced revascularisation after angiogenic stimulation in a rabbit model of bilateral limb ischaemia.

OBJECTIVE: We previously demonstrated stimulation of collateral vessel formation in a rabbit model of unilateral limb ischaemia after administration of endothelial cell growth factor (ECGF). To distinguish clearly the effects of ischaemia alone from those of ischaemia combined with angiogenic stimulation in the same animal, a model of bilateral hindlimb ischaemia was used to evaluate further the angiogenic effect of ECGF. DESIGN: Ischaemia was produced in both hindlimbs of 11 rabbits by femoral artery excision. Beginning 10 days later, ECGF (8 mg in 3 ml of saline) was injected in one hindlimb while 3 ml of saline alone was injected in the other every other day for a total of five doses. OUTCOME MEASURES: Calf systolic blood pressure was measured in both limbs on postoperative days, 10, 30, and 50. On day 50, collateral formation was quantitated angiographically, and muscle samples were obtained for quantitation of capillary density and histologic studies. RESULTS: The mean calf systolic blood pressure in the both hindlimbs was similar on day 10 (36.9 +/- 2.3 versus 38.1 +/- 2.9 mmHg) but was significantly higher in the ECGF-treated limb on day 30 (68.9 +/- 3.1 versus 45.0 +/- 2.9 mmHg) and day 50 (83.0 +/- 3.0 versus 57.0 +/- 1.7; p < 0.0001 for both comparisons). On day 50, collateral vessels were significantly more numerous in the ECGF-treated limb (17.2 +/- 1.6 versus 11.0 +/- 0.8; p < 0.0006), as were capillaries (225.9 +/- 11.4 versus 159.6 +/- 12.9 per mm2; p < 0.002). CONCLUSION: Local administration of ECGF enhanced collateral development leading to significantly improved perfusion in the treated as compared with the untreated limb in the same animal. Exogenous administration of an angiogenic mitogen can upregulate the normal collateral response to ischaemia and may be useful in treating severe limb ischaemia.

Angiography↗

Effect of addition of dried healthy or diseased parsnip root tissue to a modified AIN-76A diet on cell proliferation and histopathology in the liver, oesophagus and forestomach of male Swiss Webster mice.

Umbelliferous crop plants, including the parsnip (Pastinaca sativa L.), elaborate enhanced levels of furocoumarins, including psoralens, when subjected to biotic or abiotic stress. These furocoumarins are recognized to lead to phototoxicity. In this study, the effect of these agents, which are present in diseased parsnip root tissue, on the liver and two tissues on the route of entry to the body (the oesophagus and forestomach) were investigated. Young male Swiss Webster mice were fed for approximately 30 days with modified AIN-76A diets containing 32.5% dried healthy, 32.5% apparently healthy or 32.5% fungicide-treated parsnip root tissue, and 8, 16 or 32.5% dried diseased (Phoma complanata-infected) parsnip root tissue. As controls, three modified AIN-76A diets differing in their edible starch-to-sucrose ratios (C1-C3) were administered for an equal time. Dried healthy parsnip root tissue, compared with controls, did not significantly affect any of the indices of cellular proliferation or histopathological parameters that were assessed. Histopathological examination of the oesophagus and forestomach demonstrated no significant changes as a result of feeding any of the diets containing parsnip tissue. In the liver, the highest level (but neither of the two lower levels) of dried diseased parsnip root tissue led to swelling of the cytoplasm in cells surrounding the central vein of hepatic lobules, with consequent compression of the peripheral cells. Using [3H]thymidine radioautography, a dose-related increase in cell labelling with the level of diseased parsnip root tissue was demonstrated in the liver. Compared with control diet C2 only, the extent of [3H]thymidine labelling in the liver was increased in mice receiving apparently healthy parsnip tissue; a slight, not statistically significant, increase was also noted with fungicide-treated parsnip tissue. Increased [3H]thymidine labelling with the feeding of diseased parsnip tissue was also found in the greater curvature of the forestomach and the region of the oesophageal-forestomach junction, but not at the glandular junction of the forestomach nor in the mid-oesophagus.

Animal Feed↗

Angiogenic growth factor and revascularization of the ischemic limb: evaluation in a rabbit model.

We have previously shown that administration of endothelial cell growth factor (ECGF) significantly accelerates revascularization in the ischemic rabbit hindlimb model. Nevertheless, much remains to be learned as to the effectiveness and limitations of this approach. The present study was designed, therefore, to determine whether a dose-response relationship could be demonstrated for this agent, whether it was effective if systemically administered, and finally, whether it affected vascularization in a nonischemic limb. Our established unilateral ischemic rabbit hindlimb model and ECGF administration protocol were used to examine these questions. Three groups of animals were studied to determine a dose-response relationship of ECGF in the ischemic limb. Revascularization was assessed by measurement of calf blood left to right (L/R) pressure ratio before (Day 0) and after ECGF injections (Days 1, 10, and 20) and quantitative vascularization was assessed by angiography at Day 20 when the study was terminated. The results of the dose-response study were [formula: see text] Two other groups of animals were also studied. In Group 4, intramuscular ECGF was injected in the left front limb remote from the ischemic hindlimb and in Group 5 it was injected into the left hindlimb of a normal animal. In neither group was any significant effect on vascularization evident. Thus, our data suggest that the relationship of ECGF and revascularization of the ischemic limb is dose dependent and is demonstrable only when it is administered directly into the limb in the presence of ischemia.

Angiogenesis Inducing Agents↗

Enhanced revascularization of the ischemic limb by angiogenic therapy.

BACKGROUND: This study tests the efficacy of an angiogenic growth factor, endothelial cell growth factor, in a rabbit model of persistent hindlimb ischemia. METHODS AND RESULTS: Ischemia was induced in the left hindlimb of 22 New Zealand White rabbits by ligation of the distal external iliac artery and complete excision of the common and superficial femoral arteries. Two groups of animals were studied: Group 1 consisted of 11 animals who for 10 days received daily intramuscular injections of 4 mg of endothelial cell growth factor beginning on postoperative day 11, and group 2 consisted of 11 animals who underwent the same surgical ischemic procedure but received only injections of saline daily for the same postoperative period. Perfusion of the ischemic left limb was compared with the normal right limb in each animal on postoperative days 10, 20, 30, and 40 using the calf blood pressure ratio, 99mTc macroaggregate radioisotopic perfusion scans, and serial angiography. Neovascularization in the left thigh at day 40 was quantified from the angiograms. Each technique documented that animals in group 1 had significantly better perfusion than animals in group 2; that is, the calf pressure ratio was higher in group 1 than in group 2 (0.56 versus 0.32 at day 20, 0.64 versus 0.44 at day 30, and 0.70 versus 0.50, P < .0001), and the calf radioisotopic perfusion ratio was also higher in group 1 than in group 2 (0.88 versus 0.74 at day 20, P < .02; 0.93 versus 0.76 at day 30, and 0.96 versus 0.79 at day 40, P < .008). Angiographic studies correlated well with these results demonstrating much earlier distal arterial reconstitution and enhanced neovascularization (23.8 versus 9.0 vessels, P < .007). CONCLUSIONS: The data clearly indicate that an angiogenic growth factor, endothelial cell growth factor, promotes revascularization in this experimental ischemic hindlimb model, raising the possibility that in the future such agents might be of value in humans.

Angiography↗

Relationship between dietary fiber levels and protein digestibility in selected foods as determined in rats.

Samples of 15 food products and feces obtained by feeding them to rats were analysed for dietary fiber fractions. The food products were added as the sole source of protein in 8% protein diets, making up 8.8-51.6% of the diets. Diets were supplemented with 0.54-5.00% purified cellulose to make them more comparable in total fiber. Fiber analyses of food products revealed that the protein sources provided 0.06-7.27% total dietary fiber. The true protein digestibility in rats was negatively correlated with the total food fiber level (r = -0.69, P less than 0.01) or with the food cellulose level (r = -0.82, P less than 0.01) but it was positively correlated (r = +0.81, P less than 0.01) with the purified cellulose level. No relationship was found between protein digestibility and fiber fermentability. Results indicate that several food fiber fractions and possibly associated substances influenced protein digestibility. Purified cellulose did not have the same physiological behavior as food cellulose from the viewpoint of protein digestibility and fiber fermentability.

Animals↗

Pyogenic spondylodiscitis without disc space collapse.

An unusual patient with pyogenic spondylodiscitis and preservation of the disc space anteriorly is reported. The diagnosis of pyogenic spondylodiscitis may be difficult when there is underlying spinal disease. We emphasize the need for early systematic imaging studies and intervention in such patients, including careful evaluation of vertebral endplates on plain films, early use of radionuclide studies, and prompt needle aspiration.

Aged↗

Automated and manual determination of N1-methylnicotinamide in urine.

An automated AutoAnalyzer method and a manual adaptation of the automated method are described for determining N1-methylnicotinamide (NMN) in urine. The new methods incorporate improvements presented by Pelletier and Campbell (Anal. Biochem. 3:60, 1962) for the fluorometric determination of NMN after condensation with methyl ethyl ketone (MEK). The new methods are simpler and not limited by the usage of a single brand of MEK. Various brands of MEK into which hydrogen peroxide had been added in order to catalyze the formation of the condensation product were used and found to give comparable NMN values with different urine specimens. Results of the analysis of 21 urine specimens by the new procedures agreed well with those by the method of Carpenter and Kodicek (Biochem. J. 46: 421, 1950) for which interpretation guidelines exist. Furthermore, it was found that eluates from the chromatographic purification of thiamine in urine with Decalso retained the NMN present in the original specimens, gave complete recoveries of added NMN and were free from nearly all interfering fluorescing substances; this purification would be advantageous for determining with accuracy and precision NMN levels in the urine of deficient subjects and could be used routinely when thiamine analysis has to be performed.

Autoanalysis↗

Automated analysis of vitamin C in pharmaceutical products.

The determination of total vitamin C in the form of both l-ascorbic acid (AA) and dehydroascorbic acid (DHAA) present in pharmaceutical preparations has been automated. Total vitamin C (completely oxidized to DHAA) was determined by reaction with 2,4-dinitrophenylhydrazine while blanks utilized the same reagent after reducing all DHAA to AA. The automated method was applicable to a variety of multivitamin preparations including those containing iron and copper. The mean recovery of L-ascorbic acid added to 11 multivitamin preparations was 99.4% with a coefficient of variation of 2.5%. In the analysis of these products, results obtained by the automated method were essentially the same as those obtained by the original manual method. For preparations containing no copper salts, the results were also comparable to those obtainable by titration with 2,6-dichloroindophenol except in 1 product which contained some DHAA.

Ascorbic Acid↗