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Biomedical subjects

R Buck

Publications and source records attributed to R Buck.

At least 37 records · Page 2Linked to original sources

Social facilitation and inhibition of emotional expression and communication.

Does the presence of others facilitate or inhibit emotional expression? Female "senders" (n = 45) viewed 12 emotionally loaded slides either alone or with another sender while responses were secretly videotaped. In Study 1, 14 "receivers" guessed the type of slide viewed by dyads more accurately (eta = .366). In Study 2, 42 receivers viewed 10 senders with friends, 10 with strangers, and 10 alone. One dyad member was covered so that only 1 sender was visible. Analysis revealed significant effects of condition (alone, friend, or stranger; eta = .456), slide type (sexual, scenic, unpleasant, or unusual; eta = .325), and the Condition x Slide Type interaction (eta = .350). Strangers had overall inhibitory effects on communication accuracy, whereas friends had facilitative effects on some slides and inhibitory effects on others. Thus, both social facilitation and inhibition of expression occurred on the basis of the emotional stimulus and personal relationship involved.

Adult↗

Basic features of the polyamide membranes.

Progress in membrane and membrane process development have contributed to the continuous improvement of the extracorporeal treatment of renal failure during the last 40 years. Today membranes can be adapted to the specific needs required by their clinical application. Synthetic structures like the polyamide membranes offer a wide range of possibilities in performance and hemocompatibility, due to: (1) the use of block polymers or polymer blends and mixtures, and (2) modification of membrane structure, pore size and porosity, due to changes of the preparation process. However, further improvements are still required to achieve the ultimate goal of matching the performance of biological membranes.

Diffusion↗

Evaluation of renal function and fluid homeostasis during recovery from exercise-induced hyponatremia.

Renal function including fluid and electrolyte balance was studied during recovery in eight subjects who developed symptomatic hyponatremia (HN; plasma sodium concentration less than 130 mM) during an 88-km ultramarathon footrace and compared with results for normonatremic runners [NN; n = 18, mean postrace plasma sodium concentration, 138.2 +/- 1.2 (SE) mM]. Estimated fluid intake during the race for HN was 12.5 +/- 1.6 (SE) liters over 9 h 41 min (+/- 28 min). HN excreted a net fluid excess of 2.95 +/- 0.56 (range 1.2-5.9) liters compared with a fluid deficit of 2.7 +/- 0.3% body weight in NN. The sodium deficit was 153 +/- 35 mmol in HN and 187 +/- 37 mmol in NN. Despite the fluid overload, plasma volume was decreased by 24.1 +/- 5.0% in HN compared with 8.2 +/- 2.6% in NN. Serum renin activity (5.1 +/- 2.0 ng.ml-1.h-1), aldosterone concentrations (410 +/- 34 ng/l), creatinine clearances (174.8 +/- 28.2 ml/min), and urine output (6.4 +/- 1.0 ml/min) were markedly elevated in HN during recovery. Thus the hyponatremia of exercise results from fluid retention in subjects who ingest abnormally large fluid volumes during prolonged exercise.

Adult↗

Cultured endothelial cells from lymphatics of nude mice parasitized by Brugia malayi.

Endothelial cells from dilated inguinal lymphatics of congenitally athymic nude mice, parasitized by adult Brugia malayi, were placed in culture. Cells formed a loose monolayer and exhibited a typical cobblestone appearance. When microfilariae were present in cultures, they frequently appeared to be attached to the monolayer by one end. Approximately 75% of the primary explant cells were positive for Factor VIII-associated antigen, comparable to bovine artery endothelial cells used as a control. With few exceptions, cultures were uncontaminated with fibroblasts or other non-endothelial cell types. Large granular cells with characteristics of mononuclear/macrophage cells appeared in long term and unpassaged cultures. Cells remained viable in culture for an average of 60 days, 5 to 6 passages, before becoming highly vacuolated and assuming a rounded configuration. Viability of the cells was dependent upon heparin, serum and endothelial cell growth factor. The propensity of the lymphatics of nude mice to become greatly dilated in the presence of viable adult worms of B. malayi will prove to be important not only for the study of the effects of the parasite and its products upon endothelial cells, but also because a source of murine lymphatic endothelial cells can be readily available for functional studies.

Animals↗

Using FACS vs. communication scores to measure spontaneous facial expression of emotion in brain-damaged patients: a reply to Mammucari et al. (1988).

Buck and Duffy (1980) and Borod et al. (1985) found evidence of deficits in spontaneous expressiveness in right brain-damaged (RBD) patients relative to LBD patients and controls. Using FACS, Mammucari et al. (1988) failed to replicate this result and questioned our methods and findings. This paper replies (a) that Mammucari et al. (1988)'s review of our work is selective and misleading; (b) that there are aspects of their study that can account for their null results, including the insufficient sensitivity of FACS for the measurement of spontaneous expressiveness; and (c) that the results of Mammucari et al. (1988) regarding "aversive eye movements" to a negative film in LBD and control, but not RBD, patients are in fact compatible with our findings. This paper also suggests a general strategy for the objective and comprehensive analysis of spontaneous emotional expressiveness.

Brain Damage, Chronic↗

Relative contributions of expressive behavior and contextual information to the judgment of the emotional state of another.

This study used a technique for assessing the relative impact of facial-gestural expressions, as opposed to contextual information regarding the elicitor and situation, on the judgment of emotion. In Study 1, 28 undergraduates rated videotapes of spontaneous facial-gestural expressions and separately rated the emotionally loaded color slides that elicited those expressions. The source clarities of the expressions and slides were matched using correlation and distance measures, and 18 expressions and 9 slides were selected. In Study 2, 72 undergraduate receivers were shown systematic pairings of these expressions and slides and rated the emotional state of the expresser, who was supposedly watching that slide under public or private situational conditions. Expressions were found to be more important sources for all emotion judgments. For female receivers slides were relatively more important in the public than in the private situation.

Adult↗

Emotion, reason, and communication in coping with cancer.

Researchers have sought to identify which coping styles are most conducive to recovery from cancer and other serious illnesses. The results have been equivocal. It appears that what helps one patient does not necessarily help another. This article proposes a model explaining how emotional, cognitive, and environmental conditions influence the styles of coping used by cancer patients and the likelihood that particular styles will prove helpful to these patients. The model is based on the premise that coping does not occur in isolation from the emotional makeup, cognitive predispositions, and social environment of the patient. It describes how these processes interact to influence the protective effect of various coping styles.

Journal Article↗

Nonverbal communication accuracy in married couples: relationship with marital complaints.

Nonverbal communication abilities were examined as mediators of martial complaints for a sample of 48 recently married couples. Based on social penetration theory, we predicted that couples cohabiting longer would be better at decoding their partners' nonverbal expressions than couples cohabiting a shorter time, that individuals married to accurate nonverbal communicators would evidence fewer martial complaints, and that individuals with fewer marital complaints would be better nonverbal communicators. The data suggest that nonverbal decoding abilities do not covary with relationship length, although spouses were better at decoding their partners' expressions than were a panel of judges. In addition, nonverbal sensitivity to one's spouse did not covary with martial complaints, although an internal analysis suggests that wives' ability to decode husbands poorly encoded messages covaries negatively with both his and her complaints. Finally, husbands married to wives who are good encoders had fewer complaints, whereas the reverse was found to hold true for the relationship between husbands' encoding abilities on wives' complaints.

Adult↗

Cerebrospinal fluid lipids in demyelinating disease. I. Sphingolipids.

The lipid composition of CSF, serum and lymphocytes from patients without demyelinating disease (controls) as well as from patients with acute and chronic MS is analyzed. Individual lipid fractions are isolated by TLC and their fatty acid composition determined. Lipid and fatty acid composition of normal CSF resembles the results found in lymphocytes and it is deduced that CSF lipids are derived mainly from lymphocytes rather than white matter (myelin) or serum. There is an increase of CSF sphingomyelin in patients with acute MS (showing pleocytosis) which is apparently derived from disintegrated lymphocytes; there is also an increase of linoleic acid concentration which could come from serum because of dysfunction of the blood-brain barrier. The role of the CNS in contributing to CSF lipids is considered negligible both in controls and in patients with demyelination.

Demyelinating Diseases↗

Nonverbal communication of affect in brain-damaged patients.

A slide viewing technique designed to assess spontaneous nonverbal expressiveness was administered to 37 male patients including 8 left hemisphere damaged (aphasic), 10 right hemisphere damaged, 9 Parkinson's disease, and 10 non brain-damaged (control) patients. Patients watched different types of affective slides while their facial/gestural responses were videotaped. Judges watching the video tapes without audio guessed the types of slide being viewed. Results indicated that aphasic patients were equal to or more expressive than controls, while right hemisphere damaged and Parkinson's disease patients were less expressive. The possibility that spontaneous non verbal expressiveness is mediated by the right cerebral hemisphere, with the left hemisphere playing an inhibitory role, was discussed as a tentative explanation.

Affect↗

Nonverbal behavior and the theory of emotion: the facial feedback hypothesis.

The facial feedback hypothesis, that skeletal muscle feedback from facial expressions plays a causal role in regulating emotional experience and behavior, is an important part of several contemporary theories of emotion. A review of relevant research indicates that studies reporting support for this hypothesis have, without exception, used within-subjects designs and that therefore only a restricted version of the hypothesis has been tested. Also, the results of some of these studies must be questioned due to demand characteristics and other problems. It is suggested that visceral feedback may make a more direct contribution to emotional processes than facial feedback does and that the "readout" functions of facial expressions are more important than any feedback functions.

Emotions↗

[Degradation and biosynthesis of L-phenylalanine by chloridazon-degrading bacteria].

Incubating chloridazon-degrading bacteria with L-phenylalanine leads to the accumulation of L-2,3-dihydroxyphenylalanine, o-tyrosine and m-tyrosine in the medium. Incubating the bacteria with N-acetyl-L-phenylalanine leads to N-acetyl-(2,3-dihydroxyphenyl)alanine. Using phenylacetic acid as substrate leads to the accumulation of malonic acid. The products are isolated by gel chromatography and high performance liquid chromatography. 2,3-Dihydroxy-L-phenylalanine is attacked by a catechol 2,3-dioxygenase in the presence of Fe2. An unstable yellow compound is formed in this reaction. This meta-cleavage-product is again cleaved by a hydrolase, leading to aspartic acid and 4-hydroxy-2-oxovaleric acid. Both products were isolated fromthe reaction buffer by amino acid analysis and high performance liquid chromatography. The dioxygenase and hydrolase were partially purified and characterized. A new degradation pathway for phenylalanine is discussed and compared with known pathways. The enzymes chorismate mutase, prephenate dehydratase and prephenate dehydrogenase are characterized and inhibition as well as repression are investigated. Only prephenate dehydrogenase is inhibited by phenylalanine, tyrosine and tryptophane. Chorismate mutase is repressed by phenylalanine, prephenate dehydrogenase by phenylalanine and tyrosine. Prephenate dehydratase is not repressed by aromatic amino acids. Regulation of aromatic amino acid biosynthesis in connection with phenylalanine degradation is discussed.

Bacteria↗

Nonverbal communication of affect in preschool in children: relationships with personality and skin conductance.

A slide-viewing paradigm measuring the tendency to communicate accurate nonverbal messages via spontaneous facial expressions and gestures was applied to 13 male and 11 female preschoolers (aged 4-6 years). The children watched 16 emotionally loaded color slides while, unknown to them, their mothers viewed their reactions via television. The children's skin conductance (SC) was monitored during the experiment, and they had been rated by two teachers on a new scale of affect expression developed from Jones' externalizer/internalizer distinction. High communication accuracy was associated with low SC responding. Rated expressiveness was associated with high communication accuracy and low SC responding. Sex differences appeared in the pattern of relations between the affect expression scale and the measures of communication accuracy and SC response.

Affect↗