Biomedical subjects
Publications and source records attributed to R Burk.
One possible reason for the "toxic lens syndrome" may be the method of IOL sterilization used. Advantages and disadvantages of wet and dry sterilization techniques are discussed. Ethylene oxide remnants in IOL optics were measured using headspace chromatography. The influence of gamma radiation on PMMA lens optics was studied by spectrometry.
In enucleated pig eyes endothelial cell damage was demonstrated after YAG laser coagulation. The extent of the damage can be correlated to the distance between the laser focus and the endothelium, and to the energy. Similar effects and correlations can be demonstrated on intraocular implant lenses in an experimental model.
The more accurate the positioning and fixation of the intraocular lens has to be, the more important the process of gentle, but nevertheless reliable introduction of the lens into the posterior chamber becomes. New types of instruments are required for this. The special instruments needed for intracapsular implantation are discussed and their use demonstrated. Newly developed implantation forceps are described for the first time.
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The consideration for the choice of intracapsular lens-implantation are presented, and the surgical technique of the implantation of the new Binkhorst "all in the bag" lens is described in detail.
Besides the known cardiovascular effects of hemorrhagic shock, the following regular metabolic consequences can be demonstrated in rabbits: The rise in blood sugar in hemorrhagic shock increases with the amount of blood withdrawn per unit time. The rise in blood sugar is independent of the state of consciousness or Nembutal anesthesia. The characteristic rise in blood sugar lasts longer than the partial exsanguination phase. The rise in blood sugar is attributed to an increased glycogenolysis due to a reflex triggering of catecholamine secretion via the baroreceptors in hemorrhagic shock. More attention to metabolic consequences of hemorrhagic shock may possibly give rise to consequences for diagnosis and therapy of shock.
Two different experimental procedures were used to induce pancreatitis in rats: distal ligature of the common bile duct and the Pfeffer method in modification of Nevalainen. It could be shown that consumption of complement can be demonstrated even in pancreatitis induced by other than immunological models. Further examination in decomplemented rats showed the possibility of inducing acute pancreatitis by the above-mentioned models, although of less intensity. Therefore, the complement system will be an important factor for the progress and intensity of the inflammation process.
The influence of complement system in acute pancreatitis in the rat was examined. Two different experimental procedures were used to induce pancreatitis: distal choledochal ligature and the Pfeffer model as modified by NEVALAINEN. In both procedures an acute pancreatitis developed and complement depression was noted. These results showed that consumption of complement will occur even in pancreatitis induced by other than immunologic models. In a second series the same experimental procedures were used in rats that had been decomplemented by application of cobra venom factor. It was possible to produce an acute pancreatitis of moderate severity.
Twenty-seven cases of patent ductus arteriosus (PDA) in the dog, which were confirmed by cardiac catheterization, surgical exploration, or at necropsy, were reviewed. A machinery murmur, electrocardiographic evidence of left ventricular enlargement, and radiographic evidence of cardiomegaly with an aortic and a pulmonary artery dilatation were consistent features. Surgical repair was successful in 65% of the dogs. The postoperative radiographic and electrocardiographic changes are described. A concomitant congenital defect was present in 2 dogs. The epidemiologic features of 532 dogs identified as having PDA and submitted to the National Cancer Institute's Veterinary Medical Data Program were reviewed. Miniature and Toy Poodles, Pomeranians, and Shetland Sheepdogs were identified as being at high risk for PDA. An excess of females over males was noticed.
Somatic cell hybrids were generated by fusion of mouse erythroleukemia cells either to mouse L cells (B82), human fibroblasts (W1-18 VA2), or human marrow fractions enriched in erythroblasts. The hybrid cells were examined for globin gene expression by benzidine staining to detect cytoplasmic hemoglobin, and by molecular hybridization of cellular RNA to globin complementary DNA (cDNA) to detect globin messenger RNA (MRNA). The fibroblast (human or mouse) times erythroleukemia cell hybrids grown in monolayer retained most of the chromosomes of each parent. Neither cytoplasmic hemoglobin nor globin mRNA was detected in dimethylsulfoxide-treated fibroblast times erythroleukemia hybrid cells, indicating extinction of hemoglobin synthesis prior to the formation of cytoplasmic mRNA. The human marrow times mouse erythroleukemia hybrid cells grown in suspension culture contained only a few human chromosomes and exhibited low levels of hemoglobin synthesis which were amplified by 2% dimethylsulfoxide. Mouse (but not human) globin mRNA was demonstrated in these hybrid cells. The results suggest that somatic cell hybrids may be useful in searching for genetic factors which regulate activity of the globin genes.
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