Biomedical subjects

R C Ghosh

Publications and source records attributed to R C Ghosh.

4 recordsLinked to original sources

PubMed1999

The nucleotide sequence of sacbrood virus of the honey bee: an insect picorna-like virus.

We have determined the nucleotide sequence of sacbrood virus (SBV), which causes a fatal infection of honey bee larvae. The genomic RNA of SBV is longer than that of typical mammalian picornaviruses (8832 nucleotides) and contains a single, large open reading frame (179-8752) encoding a polyprotein of 2858 amino acids. Sequence comparison with other virus polyproteins revealed regions of similarity to characterized helicase, protease and RNA-dependent RNA polymerase domains; structural genes were located at the 5' terminus with non-structural genes at the 3' end. Picornavirus-like agents of insects have two distinct genomic organizations; some resemble mammalian picornaviruses with structural genes at the 5' end and non-structural genes at the 3' end, and others resemble caliciviruses in which this order is reversed; SBV thus belongs to the former type. Sequence comparison suggested that SBV is distantly related to infectious flacherie virus (IFV) of the silk worm, which possesses an RNA of similar size and gene order.

Amino Acid Sequence↗

PubMed1991

Suppression of cell-mediated immunity by purified aflatoxin B1 in broiler chicks.

The effect of purified aflatoxin B1 on cell-mediated immunity (CMI) in broiler chicks was assessed using doses of 0.3 and 1 mg/kg feed from hatching to 6 weeks of age. Total lymphocyte and T lymphocyte counts and the 2,4-dinitrofluorobenzene skin sensitivity test, graft-versus-host reaction and nitroblue tetrazolium salt reduction tests were used to evaluate CMI. Both doses of aflatoxin B1, including the apparently nontoxic dose of 0.3 mg/kg feed, caused a significant (P less than 0.05) decline in CMI. The functional activity of splenic macrophages was decreased significantly (P less than 0.05) by both doses of the toxin.

Aflatoxin B1↗

PubMedDate not supplied

Immunosuppression in broilers under experimental aflatoxicosis.

The effects of purified aflatoxin B1 on cell mediated immunity in broilers were carried out using 0.3 p.p.m. and 1 p.p.m. dose level. The percentage of acid alpha-naphthyl acetate esterase reacting lymphocytes was significantly (P less than 0.05) decreased in chicks fed 0.3 p.p.m. and 1 p.p.m. level of aflatoxin B1. The albumin and globulin values were also significantly decreased (P less than 0.05) at both dose levels.

Aflatoxin B1↗

PubMedDate not supplied

Use of tetracycline sorbate for the treatment of Aspergillus fumigatus infection in broiler chicks.

The efficacy of tetracycline sorbate (Pimafungil; contains 20% tetracycline sorbate w/v) was evaluated for treatment of Aspergillus fumigatus in naturally infected broiler chicks. The drug was administered to a group of 160 infected broiler chicks at 200 mg/l of drinking water, daily and consecutively for 5 days. Another group of 100 infected broiler chicks was kept as untreated controls. The chicks of the treated group showed appreciable improvement in clinical symptoms, mortality, body weight and recovery as shown by absence of gross and histopathological lesions and of the fungus.

Animals↗