PubMed Health⌕ Search

Biomedical subjects

R C Ullrich

Publications and source records attributed to R C Ullrich.

24 records · Page 2Linked to original sources
PubMed1988

Nucleotide base sequence of the mitochondrial COIII gene of Schizophyllum commune.

The cytochrome oxidase subunit III gene (COIII) of the Badidiomycete S. commune has been identified, cloned, and sequenced. The gene contains no introns, is AT-rich (69%) and exhibits a high degree of similarity to sequences from Ascomycetes. While most mitochondrial genes use both TGA and TGG to specify tryptophan, the COIII gene of Schizophyllum uses TGG exclusively. Translation requires no deviation from the universal code.

Amino Acid Sequence↗
PubMed1987

The isolation of specific genes from the basidiomycete Schizophyllum commune.

We have developed a routine way to isolate genes directly from the basidiomycete fungus, Schizophyllum commune. Plasmid DNA from a genomic gene library was used to isolate five specific genes by complementation of Schizophyllum mutations via transformation. The mutant strains were deficient in the ability to synthesize either adenine (ade2 and ade5), uracil (ura1, encoding orotidine-5'-phosphate decarboxylase; OMPdecase), tryptophan (trp1, encoding indole-3-glycerol phosphate synthetase; IGPS) or para aminobenzoic acid (pab1). In each case, Southern analysis revealed that transformation to prototrophy was concomitant with the integration of vector sequence into the genome of the S. commune mutant. Total DNA from transformants was restricted, religated, and used to transform E. coli. Ampicillin resistant plasmids were recovered from E. coli and tested for their ability to transform the corresponding mutant of S. commune. Plasmids complementing the ade2, ade5, pab1, trp1, and ura1 mutations were recovered.

Basidiomycota↗
PubMed1986

Isolation of the DNA sequence coding indole-3-glycerol phosphate synthetase and phosphoribosylanthranilate isomerase of Schizophyllum commune.

A Schizophyllum gene library was made in plasmid pRK9. Plasmids from this library were tested for their ability to complement several auxotrophic mutations of Escherichia coli. The goal was to isolate a Schizophyllum auxotrophic gene that could be used to transform a corresponding Schizophyllum auxotrophic mutant to prototrophy. Complementation was observed only for E. coli trpC indole 3-glycerol phosphate synthetase (IGPS) and phosphoribosyl-anthranilate isomerase (PRAI) mutations. Plasmids with a Schizophyllum sequence coding for both IGPS and PRAI activities were recovered from E. coli transformants. Expression of the Schizophyllum gene (TRP1) in E. coli is probably dependent on the Serratia marcescens promoter of plasmid pRK9. The DNA sequence containing the Schizophyllum TRP1 gene was not obviously rearranged in cloning.

Aldose-Ketose Isomerases↗
PubMed1986

Transformation of the basidiomycete, Schizophyllum commune.

Protoplasts of a Schizophyllum commune tryptophan auxotroph (trp1), deficient in indole-3-glycerol phosphate synthetase (IGPS), were transformed to trp+ with plasmid DNA containing the Schizophyllum TRP1 sequence. Efficiencies up to 30 transformants per microgram of plasmid DNA were obtained. Southern blots reveal that the transforming DNA is integrated in chromosomal DNA. The trp+ phenotype of transformants is stable in meiosis and mitosis. Transformants possess IGPS activity comparable to wild-type cells.

Basidiomycota↗
PubMed1980

Absence of short-period repetitive-sequence interspersion in the basidiomycete Schizophyllum commune.

Reassociation kinetics and electron microscopy were used to examine the organization of DNA sequences in the Basidiomycete Schizophyllum commune. Short-period interspersion of repetitive and unique sequences is absent from the DNA of this wood-rotting fungus. Instead, repetitive sequences are found predominantly in several, perhaps 16, clusters averaging 225 kilobases in length. The results of this study, the first concerning a fungus in the important fungal Class Basidiomycetidae, are discussed in relation to: sequence organization in three other species of true fungi, fungal evolution and the regulation of gene expression.

Agaricales↗