Formation of D-arabino-hexosulose-containg oligosaccharides during borate ion-exchange chromatography of lactose.
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Biomedical subjects
Publications and source records attributed to R Carubelli.
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Homogenates of HeLa cells contain neuraminidase activity. This enzyme is particle-bound, and it has a pH optimum of 4.2. Hydrocortisone-regulated cells contain two to three times as much neuraminidase as the corresponding controls. The hydrocortisone treatment also causes an increase in the cell content of beta-glucuronidase and acid deoxyribonuclease.
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HeLa S(3) cells grown in the presence of hydrocortisone contain more sialic acid than their corresponding untreated controls. The percentage of the total sialic acid present in the sedimentable fraction obtained from homogenates of cells treated with hydrocortisone is smaller than that of the corresponding fraction of control cells. In addition to N-acetylneuraminic acid, the chromatographic analysis suggests that HeLa S(3) cells probably contain trace amounts of N-glycolylneuraminic acid.
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