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Biomedical subjects

R Cevenini

Publications and source records attributed to R Cevenini.

At least 73 records · Page 4Linked to original sources
PubMed1990

In-vitro susceptibility of Borrelia burgdorferi and Borrelia hermsii to ten antimicrobial agents.

The in-vitro activity of ten antimicrobial agents against four strains of Borrelia burgdorferi originating both in the United States and Europe and against one isolate of B. hermsii was investigated. Ceftriaxone, erythromycin and roxithromycin were the most active drugs against both Borrelia species studied, with minimum bactericidal concentrations ranging from 0.015 micrograms/ml to 0.125 micrograms/ml.

Anti-Bacterial Agents↗
PubMed1989

Serological response to chlamydial infection in sheep, studied by enzyme-linked immunosorbent assay and immunoblotting.

Two enzyme-linked immunosorbent assays (ELISA) using highly purified elementary bodies (EB) of Chlamydia psittaci A/22 strain (ovine) or 6BC strain (psittacine) were set up for the detection of antichlamydial antibodies in sheep. No significant differences were observed between the two ELISAs, whereas these tests proved to be more sensitive than complement fixation test and showed a good correlation (r = 0.75) with immunofluorescence assay. The periodate treatment of chlamydial antigens modified the results of serological responses studied by ELISA, depending on the sera. The average reduction of ELISA values by periodate was 28%, ranging between 5% and 65%. The immunoblot analysis of sheep sera showed high cross reactivity between the polypeptides of A/22 and 6BC strains. However, some differences were observed. The major outer membrane protein (MOMP) of 6BC strain was recognized at different molecular weight position (40,000 kDa) in comparison with the MOMP of A/22 strain (38,000 kDa). In addition, a clear band of 97,000 kDa was detected by all sheep sera tested with A/22 strain. This band was undetectable in the blots performed with 6BC strain.

Animals↗
PubMed1989

In-vitro activity of ofloxacin against Chlamydia trachomatis.

The in-vitro activity of ofloxacin was evaluated against recently isolated Chlamydia trachomatis strains from patients suffering from non-gonococcal urethritis. The minimal inhibitory concentration (MIC) proved to be 1 mg/l against 8 of the 10 strains assayed (the MICs for the other two strains were 0.5 and 2 mg/l). The data obtained confirm that ofloxacin is active against Chlamydia trachomatis at concentrations achievable with the routine dosage regimen. The drug may thus be regarded as potentially useful for the treatment of non-gonococcal urethritis due to Chlamydia.

Chlamydia trachomatis↗
PubMed1988

Specific Epstein-Barr virus serological response in patients with nasopharyngeal carcinoma detected by immunoblotting.

The specific humoral immune response against Epstein-Barr virus (EBV) antigens in patients with nasopharyngeal carcinoma (NPC) was compared to that of patients with infectious mononucleosis (IM) and other EBV-seropositive subjects using immunoblotting technique. Almost all sera from EBV serologically associated NPC reacted reproducibly with a major group of polypeptides (four to six) of early antigen (EA) complex with molecular weights ranging from 50 kD to 58 kD, and with some additional polypeptides. Sera from IM-patients reproducibly recognized only one polypeptide of 50 kD belonging to the major group of polypeptides of EA-complex. Sera from patients with other types of head and neck cancer and relatively high levels of IgG antibody against viral capsid antigen (VCA) and EA did not react reproducibly with any of the EBV-associated proteins.

Carcinoma↗
PubMed1987

Prevalence of enteric adenovirus from acute gastroenteritis: a five year study.

Adenoviruses were detected in the stools in 459 of 3932 (11.6%) pediatric children hospitalized with acute gastroenteritis from January 1981 to December 1985. Out of the 459 adenovirus specimens 325 (8.3%) were presumptively identified as enteric adenovirus both by an adenovirus genus specific ELISA and by growth characteristics of adenovirus isolates in HEp-2 cells and in 293 cells. Enteral adenoviruses were found endemic since these viruses have been found for 5 successive years. A seasonal variation in the rates of adenovirus was also observed. Comparative data of rotavirus prevalence in the same study population are reported.

Acute Disease↗
PubMed1987

Enzyme-linked immunosorbent assay for the in-vitro detection of sensitivity of Chlamydia trachomatis to antimicrobial drugs.

A new method of testing antimicrobial activity in vitro against Chlamydia trachomatis by enzyme-linked immunosorbent assay (ELISA) was developed by using a monoclonal antibody reacting with the major outer membrane protein of C. trachomatis LGV2 serotype. ELISA was compared with standard iodine stain, with immunofluorescence assay (IFA) and immunoperoxidase assay (IPA) performed with the same monoclonal antibody as in the ELISA. The MICs and MBCs of rifampicin, oxytetracycline, erythromycin, chloramphenicol and cefazolin detected by ELISA were higher than those determined by iodine stain and slightly lower than those determined by IFA and IPA. Since ELISA was at least as informative as the previously described techniques, but more rapid and standardizable and easier to perform, the assay may be useful in measuring the antimicrobial drug susceptibility of C. trachomatis.

Anti-Bacterial Agents↗
PubMed1987

Incidence of endogenous and exogenous opportunistic fungal infections in relation to different prophylactic measures: clinical and microbiological study of 431 immunocompromised patients.

Two groups of immunocompromised patients were studied with the aim of pointing out the possibilities of antifungal prophylaxis in this type of patient. All patients received oral treatment with nystatin, but only the patients of one group were also in strict reverse isolation. It has been confirmed that chemoprophylactic treatments may control opportunistic endogenous mycoses effectually. On the contrary, only reverse isolation seems to be effective against airborne exogenous fungal infections. Because of the difficulty and high cost of this practice, different modalities for a really generalizable antifungal prophylaxis are required. At present only the detection of new systemic antifungal drugs, which are not toxic and are easy to use, seems hopeful.

Fungi↗
PubMed1986

Evaluation of antibodies to Epstein-Barr virus in Italian patients with nasopharyngeal carcinoma.

An association of Epstein-Barr virus (EBV) with nasopharyngeal carcinoma (NPC) has been established serologically in Italian patients. The finding of IgA antibodies to EBV-capsid antigen (VCA) and to early antigen (EA) showed a high degree of correlation for patients with NPC, thereby confirming previous reports. In addition, when considered together, IgA anti-VCA and IgG anti-EA antibody titres appeared to distinguish NPC-patients from those in control populations. Poorly differentiated and undifferentiated carcinomas with lymphoid cellular infiltrations showed the highest frequency of association with positive EBV serological tests.

Antibodies, Viral↗
PubMed1986

Capture-ELISA for serum IgM antibody to respiratory syncytial virus.

A four-component solid-phase capture enzyme immunoassay was set up to test for serum IgM antibody to respiratory syncytial (RS) virus and was compared with immunofluorescence assay (IFA). A total of 128 young children with acute respiratory infections were studied. Thirty-six were shown to be RS virus-positive by the detection of RS virus in nasopharyngeal secretions and 92 were RS virus-negative. A serum specimen was collected after admission to the hospital (days 0-4) and a further specimen was obtained during days 10-14. Out of 36 RS virus-positive patients, 28 (77.7%) were found to be positive for IgM by both capture-ELISA and IFA. Out of 92 RS virus-negative patients 5 (5.4%) were IgM-positive. Four false-positive results were obtained by IFA due to the presence of rheumatoid factor. The capture-ELISA was shown to be a reliable technique in detecting specific IgM antibody to RS virus.

Antibodies, Viral↗
PubMed1986

Class specific immunoglobulin response to individual polypeptides of Chlamydia trachomatis, elementary bodies, and reticulate bodies in patients with chlamydial infection.

Sera from 10 women with Chlamydia trachomatis culture positive cervicitis and sera from six men with C trachomatis positive non-gonococcal urethritis were studied for the presence of IgG, IgM, and IgA antibodies to polypeptides of C trachomatis elementary bodies and reticulate bodies using immunoblotting techniques. All the sera with IgG, IgM, or IgA immunoglobulins specific to C trachomatis recognised the major outer membrane protein (MOMP) of elementary bodies. IgG antibodies also detected several other proteins, whereas IgM immunoglobulins recognised only MOMP and proteins of 60 kD, 62 kD, and 66 kD. The IgA reacted with MOMP and the 60 kD and 62 kD proteins in elementary bodies. Class specific antibody response against the proteins of reticulate bodies was similar to that observed for elementary body antigens--with one substantial difference: no reaction was observed in the 60 kD and 62 kD positions. This suggests that 60 kD and 62 kD proteins are deficient in reticulate bodies.

Antibodies, Bacterial↗
PubMed1986

Antigenic specificity of serological response in Chlamydia trachomatis urethritis detected by immunoblotting.

Sera from 19 patients with Chlamydia trachomatis culture positive non-gonococcal urethritis were studied for the presence of antibodies to chlamydial proteins by immunoblotting. Ten C trachomatis negative patients with non-gonococcal urethritis and 10 healthy controls were also studied. Acute phase sera from C trachomatis positive patients with non-gonococcal urethritis reacted only with the major outer membrane protein whereas all the convalescent phase serum samples reacted with the major outer membrane protein and with a 60,000 and a 62,000 molecular weight protein. Some sera also reacted with a 45,000 molecular weight protein. Five of 10 convalescent phase samples from patients with C trachomatis negative non-gonococcal urethritis showed a reaction pattern comparable with that observed in convalescent sera from C trachomatis from C trachomatis positive patients with non-gonococcal urethritis. Sera from healthy seronegative subjects were negative by blotting.

Antibodies, Viral↗
PubMed1985

A two-year longitudinal study on the etiology of acute diarrhea in young children in Northern Italy.

During a 24-month period, 561 young children (6 months-2 years of age) hospitalized for acute diarrhea were studied for enteric pathogens. Patients positive for one or more pathogens were 359 (64.0%). Infection with one pathogen was found in 266 (47.4%) patients, whereas multiple infection was detected in 93 (16.6%) patients. Enteropathogens associated with disease were Rotaviruses: 150 (26.7%), Adenoviruses: 99 (17,6%), non-polio Enteroviruses: 48 (8.5%), Coronaviruses: 10 (1.8%), Parvoviruses: 5 (0.9%), Salmonella sp.: 41 (7.3%), Campylobacter fetus: 45 (8.0%), Giardia lamblia: 8 (1.4%), and "enteropathogenic" E. coli: 63 (11.2%) of which 15 (3%) produced heat labile enterotoxin. Seasonal occurrence of enteropathogens is also described.

Acute Disease↗
PubMed1985

Limits of antifungal prophylaxis by ketoconazole in leukemic patients.

Serum ketoconazole levels in leukemic patients, subjected to ketoconazole prophylaxis, were determined by an agar diffusion assay. High individual variability of drug concentration was noted. In addition, the highest drug concentration observed was less than 1 microgram/ml in 5 out of 6 patients studied. The reliability of prophylaxis of airborne opportunistic fungal infections by ketoconazole seems, therefore, to be hampered, at least in leukemics, by the too low serum concentrations of the drug. The bioassay proved to be highly reproducible and accurate as well as easy and safe to perform.

Adult↗
PubMed1984

A simple immunoperoxidase method for detecting enteric adenovirus and rotavirus in cell culture.

A technique which includes the use of indirect immunoperoxidase antibody (IPA) has been developed for detecting enteric adenovirus and rotavirus antigens in cell cultures and has been compared with immunofluorescence antibody assay (IFA). The IPA technique was as sensitive as the IFA. The number of positive cells detected by both techniques in tissue cultures was the same; false positive results were not observed. The applicability of IPA in clinical virology is discussed.

Adenoviridae↗
PubMed1984

An immunoperoxidase assay for the detection of specific IgA antibody in Epstein-Barr virus infections.

A technique using indirect immunoperoxidase antibody was developed for the detection of specific serum IgA antibody to Epstein-Barr virus capsid antigen and early antigen. The IgA technique was compared with an immunofluorescence antibody method. Epstein-Barr virus IgA antibody against viral capsid antigen was detected in all nine patients with Epstein-Barr virus associated undifferentiated nasopharyngeal carcinoma, in 13 (72.2%) of 18 patients with infectious mononucleosis, in 21 (28.3%) of 74 patients with acute lymphoblastic leukaemia, and in six (20%) of 30 patients who had recently had kidney transplants. Epstein-Barr virus IgA antibody against viral capsid antigen was also detected in four (10%) of 40 healthy subjects, but it was not found in any of 20 cord blood samples. Epstein-Barr virus IgA antibody to early antigen was detected in six (66.6%) patients with nasopharyngeal carcinoma and in two (2.7%) patients with acute lymphoblastic leukaemia. The immunoperoxidase assay for Epstein-Barr virus specific IgA was simple, reliable, and rapid and correlated well (r = 0.94) with the immunofluorescence antibody technique.

Adult↗