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R Cevenini

Publications and source records attributed to R Cevenini.

100 records · Page 6Linked to original sources
PubMed1979

[Antibodies against Chlamydia trachomatis in patients with non-gonococcal urethritis and in the healthy Italian population].

The Authors report the results of a study on the prevalence of serum antibodies against Chlamydia trachomatis among patients with non-gonococcal urethritis caused by Chlamydia trachomatis and diagnosed on the basis of the isolation of the microorganism in cell cultures, among patients with non-gonococcal urethritis of unknown etiology and in groups of healthy population, in Italy. The search for antibodies was performed both with complement-fixation tests in micro-titer system, using an antigen prepared from chicken embryo yolk sacs infected with the 6BC strain of Chlamydia psittaci, and with indirect immunofluorescence tests. The indirect immunofluorescence tests were carried out using as antigen the whole chlamydial inclusion developed in IUDR pre-treated McCoy cell cultures, grown on coverslips, and infected with the BU/434 strain of the LGV2 immunotype of Chlamydia trachomatis. The immunofluorescence tests proved to be much more sensitive than the complement fixation tests in detecting the presence of anti-chlamydial antibody both in patients with non-gonococcal urethritis and in the healthy population groups. The percentage of subjects possessing anti-chlamydial antibodies among the healthy population suggests a diffuse circulation of Chlamydia trachomatis infection. The possibility of employing the serological data in the diagnosis of the etiology of non-gonococcal urethritis is briefly discussed.

Adolescent↗
PubMedDate not supplied

Detection of Chlamydia trachomatis by a ligase chain reaction amplification method.

BACKGROUND AND OBJECTIVES: The ligase chain reaction is an in vitro DNA amplification technique that exponentially amplifies selected DNA sequences. GOAL: To evaluate a ligase chain reaction assay for the detection of Chlamydia trachomatis cryptic plasmid DNA (LCx Chlamydia) in patients routinely attending a sexually transmitted disease center in Italy. STUDY DESIGN: Urethral or cervical swabs were obtained from 501 consecutive patients (334 men and 167 women). The samples were assayed in parallel with LCx Chlamydia and conventional tissue culture; discordant results were further assayed by direct immunofluorescence and a ligase chain reaction with alternate primers. RESULTS: After resolution of discordant results, the LCx method showed a sensitivity, specificity, positive predictive value, and negative predictive value of 100%, 99.3%, 96.7%, and 100% in men; 100%, 100%, 100%, and 100% in women; and 100%, 99.5%, 97.1%, and 100% overall, respectively. By comparison, the sensitivity of tissue culture was 81.4% in men, 50% in women, and 77.6% overall. CONCLUSIONS: The automated LCx method is sensitive, fast, and accurate and represents a useful diagnostic tool for C. trachomatis infection, even in low and medium prevalence populations.

Chlamydia Infections↗
PubMedDate not supplied

Specific IgG and IgA antibodies to Chlamydia trachomatis in infertile women.

IgG, IgA, and IgM antibody titers to Chlamydia trachomatis were determined in sera of 80 infertile women and 100 controls by a single antigen (L-2) immunoperoxidase assay. The infertile women included 50 with unexplained infertility and normal hysterosalpingogram (HSG) and 30 with abnormal HSG. The control sera included 50 from primiparous and 50 from multiparous women. The prevalence of C. trachomatis IgG antibody was significantly higher in infertile women with abnormal HSG as compared with infertile patients with normal HSG and controls (87% v. 20% and 10%, respectively). The geometric mean titer (GMT) of C. trachomatis IgG antibodies of infertile women with abnormal HSG was significantly higher than those of controls (20.7 v. 5.6). A significantly higher prevalence of C. trachomatis IgA antibodies was found in infertile women with both abnormal and normal HSG than in controls (77% and 14% v. 3% respectively). No C. trachomatis IgM antibodies (less than 2) were found in any of the infertile or control groups. The possibility that serum C. trachomatis IgA antibodies may serve as a marker for early recognition of persistent C. trachomatis is discussed.

Adult↗
PubMedDate not supplied

Chlamydial infections in Italy.

Chlamydia trachomatis was present in cell cultures of specimens from 96 (32%) of 300 men with nongonococcal urethritis, but this organism was not isolated from 100 healthy volunteers matched for sex and age. The prevalence of serum antibodies to C. trachomatis was investigated with the use of a single-antigen, indirect immunofluorescence test; all patients with nongonococcal urethritis due to C. trachomatis had serum antibody against C. trachomatis, whereas only 54% of patients with nonchlamydial, nongonococcal urethritis and 22% of healthy volunteers had serum antibody. C. trachomatis was recovered from only one (0.9%) of 106 women observed in an obstetrical clinic for reasons other than genital tract infections. In a female population selected according to the presence of glandular ectopia of the cervix, C. trachomatis was isolated from nine (5.6%) of 160 women. A significantly (P less than 0.005) higher incidence of histological dysplasia was demonstrated in women presenting both glandular ectopia of the cervix and serum antibody to C. trachomatis than in women with glandular ectopia but no chlamydial antibodies. A seroepidemiological survey of a healthy population showed the presence of antibodies against C. trachomatis in 26% of the controls. The incidence was lowest (16%) among the children younger than ten years of age and highest (37%) among women older than 40 years of age.U

Adolescent↗