This study was undertaken to establish a typing scheme for Listeria monocytogenes. A total of 823 strains, isolated in France from 1958 to 1978, were studied; 69.4% of these belonged to serotype 4. Using a set of 20 phages, the lytic activity, frequency and specific character of these phages were estimated and phage typing carried out. We were able to define a phage typing system for L. monocytogenes using 12 principal phages and 3 secondary phages. In order to discriminate phages types within serotype 1, the phages 1, 3, 4, 5, 6 and 7 were used, and for phage types within serotype 4, the phages 10, 11, 14, 15, 16 and 17. Secondary phages 8, 9 and 20 had a weak lytic activity, but their specificity was very high. The lytic patterns obtained with the phages 9 and 20 were restricted to strains of serotype 5. We established a complete correlation between the lytic pattern of phages 1 to 8 and serotype 1, just as there was a correlation between the lytic pattern of phages 10 to 19 and serotype 4. Using the set of 20 phages we were able to type 645 of 823 strains of L. monocytogenes. Thus we were able to type 78.4% of all the strains examined. This percentage was very different according to the serotype of the strains tested: 88% of strains of serotype 4 and 57% of strains of serotype 1. With this set of phages 552 of 645 typable strains could be subdivided into 8 principal phagetypes: 3 types within the serotype 1 and 5 others in serotype 4. This phage typing system can be used in some epidemiological situations, in taxonomic investigations or as bacterial markers.

Animals↗

PubMedDate not supplied

Diagnosis of bronchial carcinoma on sections of paraffin-embedded sputum. Sensitivity and specificity of an alternative to routine cytology.

The diagnostic accuracy of sputum cytology for the diagnosis of bronchial carcinoma using paraffin-embedded, serially sectioned and hematoxylin and eosin-stained specimens was tested in 4,297 sputum samples from 1,889 patients, 219 of whom had bronchial carcinoma. The diagnostic sensitivity depended mainly on the number of investigated samples and was 85.4% with three sufficient sputa. The sensitivity was not influenced by the histologic types, location or TNM stage of the tumor. The specificity of the method was 99.5%. In three cases localization of sputum cytologically diagnosed bronchial carcinomas was not possible immediately (occult carcinomas, pTx); in two of these cases the bronchial carcinomas were located during follow-up. The third patient died without verification of the cytologic diagnosis. According to our results, sputum cytology on serial sections is a valuable instrument for mass screening of high-risk groups for the early detection of bronchial carcinoma. Lower sensitivities of sputum cytology in mass screening programs for the early diagnosis of lung cancer are discussed critically.

Carcinoma, Bronchogenic↗

PubMedDate not supplied

Detection of high risk patients in chronic myelogenous leukemia by DNA-image cytometry.

Peripheral blood smears from the time of diagnosis of 64 patients with chronic myelogenous leukemia (CML) in chronic phase were restained according to Feulgen. Nuclear DNA content of at least 100 myeloblasts and promyelocytes was measured by interactive DNA-cytometry using a TV-image analysis system, combined with an automated microscope. From the resulting DNA values the DNA parameters stemline ploidy, stemline shoulder fraction and "proliferative" fraction, which reflect cytogenetic and cell kinetic characteristics of CML, were derived. Each of these DNA parameters identified subgroups with a significantly different survival time and duration of chronic phase. Indicators for a good prognosis were a near-diploid stemline ploidy (median survival time 49 v. 26 months), a low percentage of nuclei in the stemline shoulder fraction (45 v. 28 months) and a high "proliferative" fraction (45 v. 23 months). Independently of clinical and hematological features, the DNA-cytometric parameters yielded additional prognostic information both in the univariate and multivariate analysis.

Adult↗

PubMedDate not supplied

Angioimmunoblastic lymphadenopathy--a malignant disease due to DNA aneuploidy.

Angioimmunoblastic lymphadenopathy (AIL) was considered by most authors in the 1970s as a non-malignant disease. However, it is widely accepted that many AIL cases would now be categorised as T-cell lymphomas of the AIL type. DNA measurements were performed on imprint smears from lymph nodes of 10 AIL patients to obtain further evidence for the malignancy of this disease. The 5c Exceeding Events (5cEE), representing the number of cells with a DNA content greater than 5c, excluding cells with 8c, 16c or 32c (+/- 12.5%), were taken as an index for aneuploid cells. 5cEE greater than or equal to 3 was considered to be evidence for aneuploidy and thus for malignancy. According to this definition of aneuploidy, malignancy was assumed in 9 cases out of 10. No aneuploid cells (5cEE = 0) were found in four patients with lymphadenitis.

Adult↗

PubMedDate not supplied

DNA-grading of prostatic carcinoma: prognostic validity and reproducibility.

The prognostic significance of the DNA-Malignancy-Grade (DNA-MG) was tested in 52 prostatic carcinoma patients in comparison with a cytomorphological grading system and the clinical staging. Papanicolaou- or MGG stained smears from transrectal aspiration biopsies were automatically restained according to Feulgen in a modified Shandon staining machine. The DNA-MG, based on the variation of the nuclear DNA content of tumor cells around the normal DNA peak, ranges on a continuous scale from 0.01 to 3.0. It was determined with a TV-image analysis system (Leitz, TAS plus), combined with an automatic microscope. The DNA-MG revealed a high prognostic validity, the clinical stage showed only a minor influence on the survival time and the cytopathologic grading system was of insufficient prognostic information. Significant differences in survival time could be demonstrated between patients with DNA-MGs of 0.01-1.5 and 1.5-3.0, as well as with DNA-MGs 1.0-2.0 and 2.0-3.0. The intra- and interobserver variations of the DNA grading system were found to be sigma = 0.014 and sigma = 0.036 respectively. The reproducibilities were 86.7% for intra- and interobserver measurements, when the continuous DNA-MG scale was divided in three groups.

Aged↗

PubMedDate not supplied

DNA-grading of malignant lymphomas. I. Prognostic significance, reproducibility and comparison with other classifications.

The prognostic significance of the DNA Malignancy Grade (DNA-MG) was tested for 83 malignant non Hodgkin lymphoma patients in comparison with three different subjective morphological classification systems (New Working Formulation for Clinical Usage, Rappaport and Kiel Classification). Monolayer smears prepared from paraffin embedded tissue and imprint smears from fresh cut lymph-nodes were investigated. Feulgen staining was performed automatically. The scalar DNA-MG was determined by rapid interactive DNA-cytometry, using an automatic microscope and a TV-image analysis system. A strong influence of the DNA-MG on length of survival was found. Compared with morphological classification systems, the DNA-MG was of greater prognostic validity as revealed by different statistical tests. Significant differences of survival probabilities between some groups with differences of 0.5 DNA-MG only were found. The interobserver reproducibility of this new prognostic index was found to be 95% within a range of +/-0.4 DNA-MG.

Adolescent↗

PubMedDate not supplied

DNA-grading of malignant lymphomas. II. Correlation with clinical parameters.

The clinical significance of the DNA-malignancy grade (DNA-MG) was tested in 90 malignant lymphoma patients. Feulgen stained imprint smears from freshly cut lymph-nodes were investigated. DNA measurements were performed using various cytophotometers. The scalar DNA-MG, ranging from 0.1 to 3.0, was calculated from the variance of the tumor cells around the 2c peak. Except for the CLL, all entities of the Kiel classification for non-Hodgkin's malignant lymphomas showed considerable variation of the DNA-MGs. The DNA-MG, the age of the patients and the response to treatment were seen to have a significant influence on the survival time of non-Hodgkin's lymphoma patients. In high grade lymphoma patients of the Kiel classification, the DNA-MG was found to provide additional prognostic information. The DNA-MG was strongly correlated with the ESR, low albumin concentration, low lymphocyte counts in peripheral blood, clinical and pathological staging, and the response to treatment.

Adolescent↗

PubMedDate not supplied

[Differentiation of "Pasteurella" and "Actinobacillus" from Enterobacteriaceae" by use of the O/129 vibriostatic agent (author's transl)].

Out of 134 Pasteurella and Actinobacillus strains studied, 132 are found to be susceptible to the O/129 vibriostatic agent with growth inhibition diameter ranging from 10 to 42 mm (86% in the range 25 to 32 mm); 46 reference strains of Enterobacteriaceae including Yersinia are resistant to the O/129 vibriostatic agent. This test can quickly differentiate between typical or atypical Enterobacteriaceae and the genus Pasteurella or Actinobacillus.

Actinobacillus↗

PubMedDate not supplied

[Value of amineptin in the treatment of different depressive states. Apropos of 112 cases treated as part of a national multi-centric study].

Amineptine is a new tricyclic compound with a grafted long aliphatic chain active on Dopamine synaptic release and as such more desinhibitor, safer and short acting. The study was coordinated by a national and 13 local counsellors. It was an open study. Inclusions criteria and results are examined, making obvious that patients responded well according to the Hamilton rating scale for depression (17 item version--16.4 points dropped at day 7th in 43%) and that it is an efficaceous antidepressant.

Adolescent↗

PubMedDate not supplied

[Venous lesions secondary to ventriculo-venous drainage].

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Cardiac Catheterization↗