Biomedical subjects
R Cone
Publications and source records attributed to R Cone.
Inhibitor of uracil-DNA glycosylase induced by bacteriophage PBS2. Purification and preliminary characterization.
A PBS2 phage-coded inhibitor of uracil-DNA glycosylase activity from Bacillus subtilis has been purified extensively and characterized preliminary. The inhibitor has a relative S value of 1.44 +/- 0.08 measured by sedimentation in 15 to 40% glycerol density gradients. It is unusually stable to heating and to the presence of sodium dodecyl sulfate and/or 8 M urea. The inhibitor has no known cofactor requirement and is active in the presence of 10 mM EDTA. Inhibitor activity is sensitive to digestion with proteinase K, but is insensitive to DNase or RNase digestion. The purified inhibitor behaves anomalously during electrophoresis in poly-acrylamide gels containing sodium dodecyl sulfate; however, experiments designed to show that the inhibitor is a glycoprotein were negative. The inhibitor clearly contains a protein required for activity, however, the possibility that some other molecular component is part of the active inhibitor cannot be excluded.
Developmental changes in ERP preceding movement are not affected by temporal aspects of the response.
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CNV during memory retrieval by normal and retarded adults.
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Partial purification and characterization of a uracil DNA N-glycosidase from Bacillus subtilis.
A uracil specific DNA N-glycosidase activity has been partially purified from crude extracts of Bacillus subtilis. The enzyme has a molecular weight of approximately 24 000 with no subunit structure. It has no requirement for any known cofactors but is inhibited in the presence of Co2+, Fe2+, or Zn2+. The enzyme is specific for uracil in single- and double-stranded deoxyribonucleopolymers and does not release free uracil from RNA or from poly(rU):poly(dA). In addition, neither Udr, dUMP, nor dUTP is recognized as substrate. The enzyme will attack small poly(dU) oligomers but the minimum size recognized as substrate is (pU)4. This enzyme may have a role in the repair (by base excision) or uracil in DNA arising either by incorporation during DNA synthesis or by deamination of cytosine in DNA.
Regional distributions of noradrenaline and dopamine in marmoset monkey brain.
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The mechanism of the degradation of DNA by streptonigrin.
The production of single strand cleavage in covalently-closed circular-DNA by the antitumour agent streptonigrin (reduced in situ by NADH) is demonstrated using the ethidium bromide fluorescence assay described previously. The degradation dependent on oxygen is completely inhibited by superoxide dismutase (EC 1.15.1.1) suggesting the intermediacy of the superoxide radical anion in the degradation. However similar complete inhibition of DNA strand breakage by catalase (EC 1.11.1.6) indicates that the hydroxyl radical (formed by interaction of superoxide with hydrogen peroxide) is the primary reactive species. Cupric ion stimulates the cleavage reaction and cobaltous ion has no effect in keeping with model studies using quinolinequinones.
Utility of the injury severity score: a confirmation.
Independent results obtained from Illinois Trauma Registry data confirm an earlier finding of a fixed, monotonic relationship between expected mortality and the Injury Severity Score (ISS) value of injuries sustained in both vehicular and nonvehicular incidents. Further results show the ISS measure to be a good indicator of hospitalization demand from accident victims and suggest that this variable can be used to control for variations in injury severity in comparative evaluations of EMS systems.
Inhibition of human lymphocyte blast transformation by streptolysin O.
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Analysis of T4 phage proteins. I. Conversion of precursor proteins into lower molecular weight peptides during normal capsid formation.
Radioisotopically labeled T4-proteins extracted from purified capsids and phage and from infected cells were separated by gel electrophoresis in the presence of sodium dodecyl sulfate and a reducing reagent. They were identified by autoradiography and by counting of the fractionated gels. Four major protein bands (F, A, D, and E) were detected in capsid and phage. These accounted for more than 90% of the total capsid protein and 70% of the phage protein (60% of the total capsid protein was in A-band). Coelectrophoresis of [(14)C]proteins from capsids and [(3)H]proteins from phage-infected cells indicated that the protein coded by gene 23 (P23) was a peptide chain approximately 25% longer than A-protein. Pulse-chase experiments combined with differential extraction indicated that conversion of P23 into A-protein and alteration of the protein coded by gene 22 (P22) appeared to be vital steps in formation of normal capsids. Mutations in several other genes known to prevent normal capsid formation inhibited conversion of P23 to A-protein and alteration of P22.
Lip, palate and face reconstruction following radical maxillectomy and orbital exenteration.
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Access performance in an emergency medical service system.
Data obtained from two different sources (the Illinois Trauma Registry and the Illinois Department of Transportation) are combined in an analysis of the relative performance of Emergency Medical Service (EMS) "access components". Access components are defined to include: discovery and notification; dispatch of help; and transportation. Results show that the majority of EMS incidents occurred near a care facility and encountered reasonable access delays. However, a number of "problem incidents" were identified which incurred long access delays despite close proximity to a care facility. Further results indicate the "problem incident" delays are due to the non-transportation components of the EMS access system.
EMS access performance: the relative cost of treatment delay.
A quantitatively defined relationship between in-system mortality and treatment delay (the total time delay between traumatic injury and initial care) is developed empirically from an emergency medical services data source. This relationship is described as an approximate indicator of the cost of treatment delay in the system studied which should be useful for resource allocation and planning.
Investigation of a new arthrographic contrast agent: Iotrol.
A new nonionic dimer (Iotrol; Schering AG) and diatrizoate meglumine-diatrizoate sodium (Renografin-60; Squibb) were compared as arthrographic agents by injecting these substances into the knees of rabbits. Three experienced arthrographers judged the image quality produced by Iotrol to be superior to that of Renografin-60. Following animal sacrifice, histologic examination of the synovium revealed a significant difference in the inflammatory response evoked by the contrast agents: Iotrol caused less inflammation. In a second group of rabbits, methylprednisolone was subcutaneously injected 24 hours before the arthrographic studies. The methylprednisolone significantly reduced the inflammation in the Renografin-60 subgroup when compared with the nonmedicated counterparts. No significant effect was noted in a like comparison with Iotrol. In addition, the administration of methylprednisolone led to a deterioration of the radiographic images. Based upon our data, we believe Iotrol is superior to Renografin-60 as an arthrographic agent.