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R Corelli

Publications and source records attributed to R Corelli.

8 recordsLinked to original sources

About AlloDerm.

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Biocompatible Materials↗

Bulk preparation and crystallization of the Escherichia coli elongation factor Tu-Ts complex.

A simple procedure for the preparation of 10-500 mg of the Escherichia coli elongation Tu-Ts complex is described. The protocol is based on the separate purification and quantitation of EF-Tu-GDP and EF-Ts, followed by mixing of equimolar amounts of each protein and removal of the displaced GDP by dialysis. Single crystals grown from the final product have been analyzed by X-ray diffraction techniques. The procedure is also applicable to the bulk preparation and crystallization of the trypsin-modified Tu-Ts complex. Quantitation of the elongation factors by three methods is presented.

Chromatography, Gel↗