Biomedical subjects
Publications and source records attributed to R Croft.
The existence of chronic neuropathic pain in treated leprosy has received scant attention. We describe the clinical findings of 16 patients with multibacillary leprosy who had chronic stimulus-independent pain despite finishing their treatment. With confirmation, our results could be of importance in the establishment of "care after cure" programmes for patients with leprosy.
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Many coaches use field tests to estimate athlete's aerobic capacity. Recently it has been has suggested that the 5 minute run an accurate estimator of aerobic capacity as is the 15 minute aerobic run. The aim of this experiment was to determine whether the 5 minute run was as accurate an indicator of aerobic capacity as the 15 minute run. Also we wished to determine some correlational values between measured VO2max and both the 15 minute and 5 minute runs as well as determining some regression equations to calculate VO2max (mls.kg-1.min-1) given the distance run in the 5 or 15 minute runs. We found that the 15 minute run was still the best indicator of aerobic capacity for all age groups (r = 0.881, 851, 671 and 881 for 12, 13, 14, and 15 years respectively). The 5 minute run correlated significantly with the 15 minute run and with VO2max, in all cases. Correlational values were all moderate to high in nature with no correlation being lower than r = 0.534. We would conclude that the best method to estimate aerobic capacity in a field situation is to use the 15 minute run.
We have cloned into Escherichia coli the genes for 38 type-II bacterial modification methyltransferases. The clones were isolated by selecting in vitro for protectively modified recombinants. Most of the clones modify their DNA fully but a substantial number modify only partially. In approximately one-half of the clones, the genes for the corresponding endonucleases are also present. Some of these clones restrict infecting phages and others do not. Clones carrying endonuclease genes but lacking methyltransferase genes have been found, in several instances, to be viable.
The HaeIII methyltransferase (MTase) gene from Haemophilus aegyptius (recognition sequence: 5'-GGCC-3') was cloned into Escherichia coli in the plasmid vector pBR322. The gene was isolated on a single EcoRI fragment and on a single HindIII fragment. Clones carrying additional adjacent fragments were found to code also for the HaeII restriction endonuclease and HaeII modification MTase (recognition sequence: 5'-PuGCGCPy-3'). The sequence of the HaeIII modification gene was determined. The inferred amino acid sequence of the protein was found to share extensive similarity with other sequenced m5C-MTases. The central 'non-conserved' region of the M.HaeIII MTase, thought to form the nucleotide sequence-specificity domain, is almost identical to that of the M.BsuRI, M.BspRI and M.NgoPII MTases, which also recognize the sequence 5'-GGCC-3'.