Biomedical subjects
R Cuff
Publications and source records attributed to R Cuff.
Immunochemical detection of arylamine N-acetyltransferase in normal and neoplastic bladder.
The N-acetyltransferase (NAT) phenotype is an important determinant of individual susceptibility to occupational bladder cancer. N-Acetyltransferases arc known to metabolize aromatic amine bladder carcinogens, but the functional significance of NAT expression in the target organ is unclear. To resolve this issue, polygonal antisera against purified recombinant enzymes and C-terminal peptides of human NAT Type 1 (NAT1) and Type 2 (NAT2) were generated. Western blot analysis of exfoliated cells from human urine, pig bladder homogenate, and human bladder tumor-derived cell lines showed that NAT1 was expressed in all three systems, whereas NAT2 did not appear to be expressed in the bladder. Immunohistochemical analysis of human bladder tumor sections indicated that well-differentiated tumor cells expressed NAT1, with the highest level of expression being found in the umbrella cells that line the bladder lumen. Poorly differentiated tumor regions appeared to express NAT1 at lower levels than did well-differentiated areas. These findings support the hypothesis that aromatic amines are metabolized in the bladder epithelium by NAT1.
How biomedical investigators use library books.
Relatively few studies have been concerned with the use of biomedical books. This paper reports an investigation into use made of library books by biomedical investigators. Based on cancelled charge slips collected at the Yale Medical Library circulation desk, telephone appointments were made to interview those research investigators whose books had been returned the previous day. The interviewer obtained answers from the investigator to a questionnaire to discover how the investigator had learned of a book, if the book had been useful, and, if useful, how it had been used. During the six-month study period, 30.4 percent of researchers' volumes returned were monographs. Almost four-fifths of books borrowed supplied information wanted, and about four-fifths of books used had been printed in the previous decade. Nine-tenths of the use of books was research-related, the other tenth being for lecture preparation.