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Biomedical subjects

R Cybulska

Publications and source records attributed to R Cybulska.

13 recordsLinked to original sources
PubMed1989

The development of activity of succinate dehydrogenase (EC.1.3.99.1) in the areas of the nigro-striatal pathway during the ontogenesis of a pig.

The development of activity of succinate dehydrogenase was investigated by histochemical methods under the light and electron microscopes and also by biochemical methods. The studies were carried out in the areas of the nigro-striatal pathways of 7 foetal groups, two day-old piglets, three week-old piglets and adult pigs. It was demonstrated that the activity of succinate dehydrogenase appeared first in foetuses of about 86 days (180 mm). The highest activity in prenatal development appeared just before birth in about 112 days old (260 mm) foetuses. After birth in 2 day-old piglets the activity of the examined enzyme decreases, while in 3 week-old it increases however not reaching the activity level observed in adult pigs.

Aging↗
PubMed1988

Effect of chloramphenicol on the activity of alkaline phosphatase (EC 3.1.3.1.) and the structure of microvilli of the epithelial cells of the small intestine of the hen.

Effect of a therapeutic dose of chloramphenicol (detreomycin) on the intensity of reaction of alkaline phosphatase and on the structure of microvilli of the epithelial cells of the small intestine of the hen was investigated. It was observed that this antibiotic given orally weakened the intensity of the reaction of alkaline phosphatase and changes also the shape of microvilli of intestinal epithelium. 4 weeks after chloramphenicol administration the activity of the enzyme appeared to be normal and the morphology restored. We concluded from the experiments performed, that chloraphenicol may inhibit the process of protein glycosylation in the enterocytes of the small intestine and also weakened reversible the process of absorption.

Alkaline Phosphatase↗
PubMed1981

The haloperidol effect on the histofluorescence in the caudate nucleus of the rat brain treated with agonistic and antagonistic acetylcholine compounds.

The aim of the paper was to trace the haloperidol effect in the acute and chronic experiments on the histofluorescence in the caudate nucleus of the rat and in conditions of pretreatment of those animals with agonistic and antagonistic compounds of acetylcholine. The experiments were performed on 35 Wistar rats of 180 g mean body weight. According to the experimental model the following were administered: alpha-MT; alpha-MT + haloperidol; Haloperidol in single dose and in repeated multiple doses; Atropine: Atropine + haloperidol; carbachol and carbachol + haloperidol. The Falck et al. [5] histofluorescence method was applied to demonstrate catecholaminergic structures. Changes in the fluorescence of nucleus caudatus of the rat after single or repeated administration of haloperidol and after combinations with cholinergic compounds were always accompanied by the decrease of histofluorescence. We assume that it can be accounted for the existence of functional bonds between the dopaminergic and the cholinergic systems. Those bonds can be explained on the structural grounds, that is by a dense network of nerve endings and in bundles of preterminal nerve fibres. Neurocytes bodies were not sensitive to the administered drugs. The dense network of nerve endings proved to be most sensitive to the action of haloperidol given by itself and in combination with other drugs. A single large dose of haloperidol produced more pronounced effects than several repeated doses of that neuroleptic.

Animals↗
PubMed1980

Activity of acetylcholine hydrolase (EC 3.1.1.7) in the pig brain; spectrophotometric and histochemical studies in the light and electron microscope.

AChE activity (EC 3.1.1.7) in the pig brain was examined by spectrophotometric method and histochemically in the light and electron microscope. The results obtained correlate to a high degree with the existing data concerning the activity of the studied enzyme in other mammals. In the pig, the highest activity was encountered in the striatum nucleus accumbens and nucleus amygdale lateralis: the lowest activity was found in encephalon and cerebellum. In comparison with other mammals AChE activity in the pig brain is considerably lower. No comparable proportions have been encountered between the activities of AChE and ChAT in particular brain regions in the mammals examined so far and also in the pig. The results of histochemical examination both in the light and in the electron microscope correspond to the results obtained by other authors. The discussion deals with the problem of correlation between the level of AChE activity in particular regions of the pig brain with respect to their phylogenetic growth.

Acetylcholinesterase↗
PubMed1978

Interaction between cholinotropic and adrenotropic drugs on the histofluorescence of the central catecholamine structures in the rat.

Male Wistar rats were given phentolamine or phenoxybenzamine in the lateral ventricles in doses of 1 mg/kg. After 30 minutes they received in the same way atropine in doses of 1 mg/kg or carbachol in doses of 0.05 mg/kg. The control group was given physiological saline. The animals were decapitated 30 minutes after drug administration. The Falck and Hillarp histofluorescence method was applied. The areas of DA (nigrostriatal and meso-limbic) and NA systems were examined. It was found, that atropine increased the intensity of fluorescence in comparison with the control group, in all areas of DA structures. The action of carbachol was more differentiated. In the substantia nigra (A8 and A9) respectively in the globus pallidus and the nucleus arcuatus (A12) its effect was the same as that of atropine. In other areas it caused weakening of fluorescence or showed no effect. In the NA system atropine weakened the fluorescence considerably while carbachol increased it in five out of eleven areas. The interaction of cholinotropic and adrenotropic drugs is disscused.

Animals↗
PubMed1976

Influence of atropine and carbachol on the fluorescence of catecholaminergic structures in selected areas of the rat brain.

Using the formaldehyde-fluorescence technique, the authors studied the influence of atropine and carbachol, administered intraventricularly to Wistar rats, on the fluorescence of catecholaminergic structures in 20 areas of the CNS, situated within the range of the 10th-46th frontal plane according to KONIG and KLIPPEL. 1. A confirmation of the antagonistic action of atropine and carbachol was obtained. It was expressed by mutually opposed occurrence of the specific fluorescence of the catecholaminergic structures. 2. In 16 out of 20 studied areas of the CNS, carbachol abolished or considerably weakened the specific fluorescence. In 3 areas it was increased by this drug, and one area proved insensitive. 3. Atropine increased the specific fluorescence in the DA (dopaminergic system) areas, while it had varying effects in the NA (noradrenergic system areas. In some areas of the CNS it increased and in others reduced the specific fluorescence of the catecholaminergic structures. 4. An interference between atropine and carbachol is observed, but it seems that the results of the present experiment speak in favour of an interaction between the catecholamine transmitters and ACh in the particular areas of the CNS under the influence of atropine and carbachol. 5. The authors discuss in detail the reactions of the catecholaminergic structures in the particular areas of the CNS, in which, as compared with the control, an increase or a decrease of the specific fluorescence under the influence of the administered drugs was observed.

Animals↗
PubMed1975

The influence of phentolamine on the fluorescence of catecholaminergic structures in selected areas of rat brain.

Wistar rats were given phentolamine into the ventriculus lateralis. The D1 group of rats received a larger dose than the D2 group. The animals were decapitated within 2 hours after phentolamine injection. The FALCK fluorescence technique was applied to demonstrate the fluorescence of catecholaminergic structures. The results in rat brain areas selected according to KONIG and KLIPPEL are shown in figs 10-46. Whereas earlier biochemical experiments did not show any phentolamine-induced changes in the NA level of the whole brain, the present histochemical experiments carried out with the fluorescence technique revealed the influence of phentolamine on individual structures and areas of the NA system. In comparison with the control material in 5 out of 11 areas the fluorescence was much weaker, in 3 it was similar to the control group, and in 3 (1 with a larger dose and 2 with a smaller dose of phentolamine) it was slightly stronger. The simultaneous increase of fluorescence in 6 out of 7 areas of the DA system may indicate a compensatory interaction of these areas as a response to the neuromediator decrease in the NA system. The paper discusses the increase in the intensity of fluorescence induced by a small dose of phentolamine in some brain areas, or by a large dose in others, both these alternatives depending on the neuromediator turnover.

Animals↗
PubMed1975

Attempts at differentiation of the structure of neurons in the corpus geniculatum laterale of the pig by the immunocytochemical technique.

The investigations were carried out on the corpus geniculatum laterale of the pig with the aim to differentiate the structure of its neurons on the basis of the antigenic properties of the nervous tissue. The antigenic material consisted of the homogenate of the corpus geniculatum laterale (H) and of its nuclear (P1) and mitochondrial (P2) fractions. The antigens were used to immunize 3 groups of rabbits, the 4th one serving as control. The antigens were prepared according to requirements of the technique. By immunization of the rabbits with the antigens immune sera were obtained. These sera were processed in accordance with the requirements of the technique. The authors conducted the studies employing the immunofluorescent technique after Coons with the use of the anti-rabbit IgG goat serum conjugated with fluorescein, as well as the immunohistochemical method according to Nakane with the use of the antibodies labelled with horseradish peroxidase. The investigations led the authors to conclude that H as well as P1 and P2 possess distinct antigenic traits. Moreover, the findings of the immunofluorescent and immunohistochemical tests in which the anti-H serum was used point to the localization of the antigen in the following nerve structures: neuron perikaryons, glia cells, and nerve fibres. On the other hand, the application of anti-P1 and anti-P2 sera permitted the antigens to be localized in the peripheral areas of the neuron nuclei and in the mitochondria. The results obtained with the immunofluorescent technique were in agreement with those produced immunohistochemically.

Animals↗
PubMedDate not supplied

The locomotor and stereotype response to dopaminergic drugs and caffeine after intracerebroventricular kainic acid in rats.

The effects of single kainic acid (KA) (0.1 microgram) intracerebroventricular injection on locomotor and stereotypy responses to amphetamine and apomorphine, and on locomotor response to caffeine were measured at different intervals (24, 120, 240, 480 h). The responses to amphetamine and caffeine were markedly elevated during the all period. In contrast, the responses to apomorphine remained unchanged. Histological examination showed severe destructions of neurons and fibres within the nucleus caudatus and the permanent loss of hippocampal pyramidal cells.

Amphetamine↗