Biomedical subjects
Publications and source records attributed to R D Broderick.
The use of a Sharpoint blade to perform anterior capsulotomy eliminates the problem of capsular tags superiorly and facilitates removal of cortical remnants. The crisp, clean edge of the superior portion of the capsulotomy enhances visualization and aids in intraocular lens placement in the capsular bag.
Sister chromatid exchange (SCE) and cell cycle progression of colony-forming cells (CFU-S) from patients with acute myelogenous leukemia were studied using bromodeoxyuridine (BrdU) incorporation and sister chromatid differential staining. The mean SCE rate of CFU-S was 7.99, which is similar to that reported for human peripheral blood lymphocytes. Cultures treated with BrdU at culture initiation and then harvested 72-120 hr later indicated a cell cycle time (TC) of approximately 60 hr. However, cells allowed to proliferate for 72-120 hr prior to the addition of BrdU showed TC values of 12-36 hr. Analysis of serial cultures from one patient revealed a heterogeneous population with a mean TC of 19.5 hr. These cell cycle times are considerably shorter than previously published reports and suggest that leukemic cells have the potential to divide rapidly.