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R D Everett

Publications and source records attributed to R D Everett.

76 records · Page 5Linked to original sources
PubMed1981

DNA replication of bacteriophage T5. 3. Studies on the structure of concatemeric T5 DNA.

The replication of bacteriophage T5 DNA has been shown to proceed via branched concatemeric intermediates. The structures of this concatemeric DNA was studied with respect to single-stranded regions and single-strand interruptions by digestion with S1 nuclease and agarose gel electrophoresis after alkali denaturation. The results were compared with the pattern of 'nicks' in the mature virion DNA, and the possible origins of these nicks are discussed. The structure of T5 concatemeric DNA was also studied by electron microscopy. Replication forks, loops and rare circular structures were observed, all of which were similar to those seen in replicating DNA of other large phages. Phage capsid structures were detected in association with both concatemeric and mature phage length DNA. These observations are discussed in relation to the replication, maturation and packaging of T5 DNA.

Capsid↗
PubMed1980

DNA replication of bacteriophage T5. 1. Fractionation of intracellular T5 DNA by agarose gel electrophoresis.

Two forms of DNA may be isolated from gently lysed T5-infected bacteria. In neutral sucrose gradients a fast sedimenting form (fsf) is distinguishable from a slow sedimenting form (ssf) which moves at a rate similar to that of DNA extracted from mature T5 virus particles. A method of agarose gel electrophoresis is described which gives complete separation of the slow sedimenting from the fast sedimenting form. The DNA was extracted from the agarose using potassium iodide and isopycnic centrifugation; samples of the slow sedimenting form prepared by these methods were suitable for detailed structural studies.

Centrifugation, Density Gradient↗
PubMedDate not supplied

The regulation of transcription of viral and cellular genes by herpesvirus immediate-early gene products (review).

The expression of viral genes during infection of tissue culture cells by herpesviruses can be divided into three broad classes termed Immediate-Early (IE), early and late. The IE polypeptides include trans-acting regulators of gene expression; prior expression of IE gene products is essential for the activation of transcription from early gene promoters. During the past five years, the use of modern gene manipulation techniques, short-term transfection assays and in vitro methods has resulted in a significant increase in our understanding of the IE polypeptides. This review concentrates on the genetic, physical and functional properties of herpesvirus IE gene products with particular emphasis on those of herpes simplex virus type 1.

Animals↗