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Biomedical subjects

R D Rao

Publications and source records attributed to R D Rao.

12 recordsLinked to original sources
PubMed2004

Changes in galactose and lactic acid content of sweet whey during storage.

Whey is often stored or transported for a period of time prior to processing. During this time period, galactose and lactic acid concentrations may accumulate, reducing the quality of spray-dried whey powders in regard to stickiness and agglomeration. This study surveyed industry samples of Cheddar and mozzarella cheese whey streams to determine how galactose and lactic acid concentrations changed with storage at appropriate (4 degrees C) and abuse (37.8 degrees C) temperatures. Samples stored at 4 degrees C did not exhibit significant increases in levels of lactic acid or galactose. Mozzarella whey accumulated the greatest amount of galactose and lactic acid with storage at 37.8 degrees C. Whey samples derived from cheese made from single strains of starter culture were also evaluated to determine each culture's contribution to galactose and lactic acid production. Starter cultures evaluated included Streptococcus salivarius ssp. thermophilus. Lactobacillus helveticus, Lactobacillus delbrueckii ssp. bulgaricus, Lactococcus lactis ssp. cremoris, and Lactococcus lactis ssp. lactis. Whey derived from L. helveticus accumulated a significantly greater amount of lactic acid upon storage at 37.8 degrees C as compared with the other cultures. Galactose accumulation was significantly decreased in whey from L. lactis ssp. lactis stored at 37.8 degrees C in comparison with the other cultures. Results from this study indicate that proper storage conditions (4 degrees C) for whey prevent accumulation of galactose and lactic acid while the extent of accumulation during storage at 37.8 degrees C varies depending on the culture(s) used in cheese production.

Cheese↗
PubMed2003

Aerosol therapy for malignancy involving the lungs.

The lungs are common sites of involvement by primary and metastatic malignant disease. Patients with malignancies in the lung have limited treatment options and are usually not curable. Numerous investigators have studied the potential of delivering various therapeutic agents directly to the lungs and pulmonary lymphatics by nebulization. Most of the research involves the use of immunomodulatory strategies; a few aerosol studies of chemotherapy and gene therapy have also been conducted. Most of these studies have been conducted in animal models. A few human trials have also been completed. Results suggest that aerosol therapies have the potential to shrink pulmonary metastases of selected histologies, and that survival in selected patients with metastatic renal cell cancer may be prolonged. The approach to therapy of cancer in the lungs holds promise as a means to avoid systemic toxicity and obtain an improved therapeutic effect. Research is currently underway to address issues of local versus systemic toxicity, optimal drug delivery and selection of optimal drugs and schedules including outpatient aerosol therapy. Future issues in design of aerosol cancer treatment include identifying effective combinations of agents, schedules, and use of aerosol therapy at home as adjuvant therapy.

Administration, Inhalation↗
PubMed2001

Whiplash injury determination with conventional spine imaging and cryomicrotomy.

STUDY DESIGN: Soft tissue-related injuries to the cervical spine structures were produced by use of intact entire human cadavers undergoing rear-end impacts. Radiography, computed tomography, and cryomicrotomy techniques were used to evaluate the injury. OBJECTIVES: To replicate soft tissue injuries resulting from single input of whiplash acceleration to whole human cadavers simulating vehicular rear impacts, and to assess the ability of different modes of imaging to visualize soft tissue cervical lesions. SUMMARY OF BACKGROUND DATA: Whiplash-associated disorders such as headache and neck pain are implicated with soft tissue abnormalities to structures of the cervical spine. To the authors' best knowledge, no previous studies have been conducted to determine whether single cycle whiplash acceleration input to intact entire human cadavers can result in these soft tissue alterations. There is also a scarcity of data on the efficacy of radiography and computed tomography in assessing these injuries. METHODS: Four intact entire human cadavers underwent single whiplash acceleration (3.3 g or 4.5 g) loading by use of a whole-body sled. Pretest and posttest radiographs, computed tomography images, and sequential anatomic sections using a cryomicrotome were obtained to determine the extent of trauma to the cervical spine structures. RESULTS: Routine radiography identified the least number of lesions (one lesion in two specimens). Although computed tomography was more effective (three lesions in two specimens), trauma was not readily apparent to all soft tissues of the cervical spine. Cryomicrotome sections identified structural alterations in all four specimens to lower cervical spine components that included stretch and tear of the ligamentum flavum, anulus disruption, anterior longitudinal ligament rupture, and zygopophysial joint compromise with tear of the capsular ligaments. CONCLUSIONS: These results clearly indicate that a single application of whiplash acceleration pulse can induce soft tissue-related and ligament-related alterations to cervical spine structures. The pathologic changes identified in this study support previous observations from human volunteers observations with regard to the location of whiplash injury and may assist in the explanation of pain arising from this injury. Although computed tomography is a better imaging modality than radiography, subtle but clinically relevant injuries may be left undiagnosed with this technique. The cryomicrotome technique offers a unique procedure to understand and compare soft tissue-related injuries to the cervical anatomy caused by whiplash loading. Recognition of these injuries may advance the general knowledge of the whiplash disorder.

Aged↗
PubMed2001

Sequence information, distinction and quantitation of C-terminal leucine and isoleucine in ternary complexes of tripeptides with Cu(II) and 2,2'-bipyridine.

Tripeptides form ternary complexes with Cu(2+) and 2,2'-bipyridine (bpy) that self-assemble upon mixing the components in aqueous methanol solution. Electrospray ionization (ESI) of the complex solutions provides abundant singly charged [Cu(peptide -- H)bpy](+) and doubly charged [Cu(peptide)bpy](2+) ions. Collision-induced dissociation (CID) at low ion kinetic energies of several tripeptides, AGG, GGA, LGG, GGL, GGI, FGG, GGF, LGF, GLF, GFL, GYA and GAY, showed fragments that were indicative of the amino acid sequence in the peptide. In addition, CID of single and doubly charged complexes of isomeric tripeptides GGL and GGI provided unambiguous distinction of the isomeric leucine and isoleucine residues. Leucine peptides eliminated C(3)H(7) radicals from the amino acid side-chain whereas isoleucine eliminated C(2)H(5) radicals. CID of gas-phase doubly charged peptide complexes in a quadrupole ion trap produced a series of singly charged sequence fragments that following isolation and further CID furnished distinct fragments that allowed quantitation of leucine and isoleucine-containing peptides in mixtures.

2,2'-Dipyridyl↗
PubMed2001

Interleukin-6 and nerve growth factor levels in peripheral nerve and brainstem after trigeminal nerve injury in the rat.

Earlier studies have demonstrated that inflammation plays a role in the development of evoked pain following partial nerve injury. In this report, we demonstrate bilateral changes in interleukin-6 (IL-6) and nerve growth factor (nerve growth factor) levels following unilateral infraorbital nerve (infraorbital nerve) constriction. infraorbital nerve constriction resulted in an initial period of decreased mechanical sensitivity (1 and 3 days), followed by recovery (7 days) and then a marked bilateral mechanical hypersensitivity (10 and 28 days). nerve growth factor levels in the injured infraorbital nerve were elevated on all days, but peak concentrations of nerve growth factor were observed on day 3. A smaller increase was also observed on days 1, 3, and 7 in the uninjured nerve. A bilateral elevation of IL-6 was also seen on days 3 and 10 in the infraorbital nerve, and in the brainstem on days 3, 7 and 10 after constriction. No changes in mechanical sensitivity were found after a sham-injury, but there was a small increase in brainstem IL-6 ipsilaterally at 7 days. We conclude from these data that increases in IL-6 and nerve growth factor may contribute to the development of mechanical allodynia after trigeminal nerve injury, but they are not specifically correlated with the onset or duration of pain behaviors.

Analysis of Variance↗
PubMed2001

Late recurrence of a primitive neuro-ectodermal tumor.

Late recurrences after therapy are rare in primitive neuro-ectodermal tumor (PNET). Most recurrences occur within the first 2 years after therapy, although a small number of recurrences may occur up to 5 years after therapy. We present a rare case of a recurrence of PNET in a 31-year-old woman 17 years after her initial presentation. The potential biological implications of this late recurrence as well as responses to subsequent therapy, including temozolomide, are discussed.

Adult↗
PubMed1998

Holmium:YAG percutaneous nephrolithotomy: the laser incident angle matters.

PURPOSE: Laser physics dictate that maximal radiant exposure occurs when the laser strikes the target at a normal incidence. Since the renal collecting system often limits nephroscope movements during percutaneous nephrolithotomy, the laser-calculus incidence angle may vary. We have observed during holmium:YAG percutaneous nephrolithotomy that the side firing fiber more easily approaches a normal incidence compared to the end firing fiber. We test the hypothesis that holmium:YAG percutaneous nephrolithotomy is faster with a side firing fiber compared to an end firing fiber. MATERIALS AND METHODS: Consecutive holmium:YAG percutaneous nephrolithotomy cases were studied retrospectively. The calculus size and composition, surgical time, fragmentation speed (size/time) and stone-free rates were compared between 11 patients treated with end and 8 treated with side firing fibers. RESULTS: When the end and side firing groups were compared, calculus sizes (mean plus or minus standard deviation) were 22 +/- 13 versus 48 +/- 17 mm. (p = 0.004), calcium oxalate monohydrate incidence was 55 versus 75% (p = 0.3), surgical times were 168 +/- 87 versus 124 +/- 51 minutes, computed fragmentation speeds were 0.15 +/- 0.09 versus 0.43 +/- 0.15 mm. per minute (p = 0.0009) and stone-free rates were 73 versus 88% (p = 0.4), respectively. CONCLUSIONS: The side firing fiber is faster than the end firing fiber for holmium:YAG percutaneous nephrolithotomy. These results are consistent with principles of laser physics.

Aluminum↗
PubMed1997

Ureteroscopic management of ureteral calculi: electrohydraulic versus holmium:YAG lithotripsy.

PURPOSE: This study compared electrohydraulic and holmium:YAG lithotripsy for ureteral calculi. MATERIALS AND METHODS: Two cohorts of consecutive patients with ureteral calculi treated with ureteroscopic electrohydraulic or holmium:YAG lithotripsy were retrospectively compared. Electrohydraulic lithotripsy was done using a 1.9F fiber at energy settings between 50 and 100 v. Holmium:YAG lithotripsy was done using a 365 microm. fiber at energy settings of 0.6 to 1.5 J. RESULTS: A total of 23 and 47 consecutive patients underwent electrohydraulic and holmium:YAG lithotripsy, respectively. For preoperative calculi less than 15 mm. mean stone size plus or minus standard deviation was 9 +/- 3 versus 9 +/- 3 mm. (p = 0.5), mean operative time was 72 +/- 21 versus 102 +/- 38 minutes (p = 0.004), stone-free rate at the end of ureteroscopy was 65 versus 97 (p < 0.01) and 3-month stone-free rate was 94 versus 97% (p = 0.4) for electrohydraulic versus holmium:YAG lithotripsy. For preoperative calculi 15 mm. or greater stone size was 19 +/- 5 versus 19 +/- 4 mm. (p = 0.9), operative time was 159 +/- 61 versus 108 +/- 27 minutes (p = 0.01), stone-free rate at the end of ureteroscopy was 33 versus 87% (p = 0.001) and 3-month stone-free rate was 67 versus 100% (p = 0.02). Complications were not significantly different in either comparison. CONCLUSIONS: The overall likelihood that a patient would be rendered stone-free at ureteroscopy and 3 months after ureteroscopy favored holmium:YAG over electrohydraulic lithotripsy. For ureteral calculi less than 15 mm. electrohydraulic lithotripsy was more rapid than the holmium:YAG procedure but for ureteral calculi 15 mm. or greater the holmium:YAG technique was more rapid than electrohydraulic lithotripsy. The outcomes differences may have resulted from the different mechanisms of electrohydraulic and holmium:YAG lithotripsy.

Adult↗
PubMed1996

Carboxylate and amine terminus directed fragmentations in gaseous dipeptide complexes with copper (II) and diimine ligands formed by electrospray.

Singly and doubly charged peptide complexes with copper(II) and 2,2'-bipyridyl (bpy) are formed in the gas phase by electrospraying water-methanol solutions containing the components. Collisionally activated dissociations at low kinetic energies of singly charged complexes of the [CuII(peptide-H)(bpy)].+ type provide information about the amino acid sequence for L-Phe-Leu, L-Leu-Phe, L-Phe-Pro, L-Pro-Phe, L-Phe-Met, L-Met-Phe, L-Ser-Phe, L-Asp-Phe, and L-His-Phe. Dissociations of doubly charged complexes of the [CuII(peptide)(bpy).2+ type also allow identification of the N- and C-terminal amino acid residues. Leucine and isoleucine residues are readily distinguished in L-Ala-Leu and L-Ala-Ile through dissociations of their Cu complexes. Ion dissociation mechanisms, as elucidated by deuterium labeling, are discussed.

Amino Acid Sequence↗
PubMed1991

Quantitative differences in the histology of the attachment zones of the meniscal horns in the knee joint of man.

The attachment zones of the meniscal horns of 7 dissecting room cadavers were examined by routine histology. All the knees were devoid of gross pathological change and no discoid menisci were included. Significant differences are reported in the thickness of the zones of uncalcified fibrocartilage and cortical calcified tissue (calcified fibrocartilage and underlying lamellar bone) and in the percentage of bone:bone marrow. There was a thicker zone of uncalcified fibrocartilage and a greater quantity of calcified tissue at the horns of the lateral than the medial meniscus. The differences in uncalcified fibrocartilage were largely attributable to the posterior horns, but the variations in calcified tissue mainly reflected differences between the anterior horns. It is suggested that the greater mobility of the lateral meniscus and the blending of its anterior horn with the anterior cruciate ligament are important factors accounting for the quantitative differences in the meniscal attachment zones.

Aged↗
PubMedDate not supplied

Non-Hodgkin's tumor and Pancoast's syndrome.

A 60-year old man presented with Horner's syndrome, and acute right hand and lower extremity weakness. Chest X-ray and MRI revealed a right apical lung tumor (presumed to be a primary lung cancer), with brachial plexus infiltration and spinal cord compression. Emergent radiotherapy was initiated for spinal cord compression and a biopsy was obtained 24 h later. A careful review of pathology demonstrated a non-Hodgkin's lymphoma. The patient subsequently received chemotherapy, and is now in remission. This case illustrates the importance of a tissue diagnosis before initiating therapy for a Pancoast's tumor.

Carcinoma↗