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Biomedical subjects

R Dina

Publications and source records attributed to R Dina.

26 records · Page 2Linked to original sources
PubMed1983

Mucin secretion and morphological changes of the mucosa in non-neoplastic diseases of the colon.

Changes in mucin secretion and increase in height of the colonic mucosa adjacent to colorectal carcinoma (transitional mucosa) have been considered pre-malignant. In this study similar changes (both morphological and histochemical) have been found in some cases of ulcerative colitis and ischaemic colitis, as well as in juvenile, inflammatory and hyperplastic (metaplastic) polyps. 'Transitional' patterns of mucin secretion also occur in some other cases of ulcerative colitis, colostomies and Crohn's disease of the colon in which the mucosa has a normal height, suggesting the changes in mucin secretion are independent of mucosal morphology. In all these pathological conditions, hyperplastic (metaplastic) mucosa also coexisted. These findings seem to suggest that: (1) 'transitional' changes more likely represent a secondary regenerative phenomenon rather than a premalignant one; (2) the pattern of mucin secretion is not selective enough to serve as a premalignant marker; therefore is not a valid prognostic indicator in colonic biopsies; (3) hyperplastic (metaplastic) changes might derive from 'transitional' mucosa as a result of a more mature phase of this exaggerated regenerative phenomenon. However, in some patients longstanding 'transitional' mucosa may lead to dysplasia under the influence of environmental and genetic factors.

Biopsy↗
PubMed1983

Juvenile and inflammatory polyps of the colon--a histological and histochemical study.

A light microscopy and histochemical study of 24 juvenile and 27 inflammatory polyps showed that both may derive from inflammatory processes. Granulation tissue, secondary to spontaneous local inflammation or due to surgical procedures may subsequently be covered by regenerating epithelium which lines haemorrhagic cavities and mucus lakes to form irregular, elongated and cystic glands, which are characteristic of juvenile polyps. Both juvenile and inflammatory polyps showed cystic, metaplastic and 'transitional-type' glands. The mucin distribution was identical in both types of polyps. All these findings suggest a common origin of the polyps. The presence of 'transitional-type' glands seems to confirm these as a secondary regenerative phenomenon rather than pre-neoplastic, although dysplastic changes in juvenile polyps have been described. It is suggested that both the juvenile and inflammatory polyps may undergo dysplasia only in genetically predisposed subjects. However, this event seems to be very rare.

Adolescent↗
PubMed1982

"The evolution of the solitary ulcer of the rectum"--an endoscopic and histopathological study.

A follow-up study of fourteen out of twenty-seven cases of solitary ulcer syndrome of the rectum was performed at one year's interval from the previous endoscopic and histologic examination. Most of the lesions consistently changed their aspect, resulting in incomplete irregular narrowings, polypoid protrusions or linear ulcers. The symptoms remained unchanged or slightly diminished. A striking difference was found in the histological pattern of the "evolved" ulcer in comparison to the active one, the former showing features of hyperplastic (metaplastic) colonic polyps as well as of the so called "transitional mucosa". It is suggested that chronic ischemia occurring in solitary ulcer of the rectum leads to "transitional mucosa" as an exaggerated regenerative phenomenon, hyperplastic (metaplastic) mucosa representing a later maturative phase. Possibly "transitional" mucosa may undergo dysplastic changes under the influence of environmental factors.

Adolescent↗
PubMed1982

Transitional polyps of the colon.

Fourteen polps with the gross appearance of small hyperplastic (metaplastic) polyps, not exceeding five mm in diameter, were removed from the rectum and the sigmoid colon of patients during colonscopy, and from surgical specimens. At light microscopy they were seen to be composed of apparently normal mucosa. In most cases some hyperplastic (metaplastic) and adenomatous polyps were also present. HID-AB stain revealed the polyps to be composed of "transitional" mucosa containing almost exclusively sialomucins, in contrast to normal mucosa, in which sulphomucins predominate. It is suggested that these "transitional" polyps may represent a reservoir of hyperplastic (metaplastic) and adenomatous polps, the different fate depending on different environmental situations.

Colonic Neoplasms↗
PubMed1981

"Transitional" and hyperplastic-metaplastic mucosa occurring in solitary ulcer of the rectum.

Non-polypoid hyperplastic mucosa similar to that adjacent to colorectal carcinomas and adenomas, the so-called "transitional mucosa" (TM), was found in 16 out of 27 cases of solitary ulcer syndrome of the rectum. In 18 cases fully developed hyperplastic (metaplastic) mucosa was also found. It is suggested that chronic ischaemia which takes place in the solitary ulcer syndrome leads to TM as an exaggerated regenerative phenomenon. Later maturation of TM could lead to hyperplastic (metaplastic) changes.

Humans↗
PubMedDate not supplied

Role of esophageal brushing cytology in monitoring patients treated with sclerotherapy for esophageal varices.

Recent reports claim that there might be a relationship between sclerotherapy for esophageal varices and cancer of the esophagus. The discovery of a squamous cell cancer of the lower esophagus in a patient treated three years previously with sclerotherapy led us to set up a follow-up protocol. In order to assess this relationship and to monitor the evolution of such lesions, 68 patients treated with sclerotherapy with polidocanol because of esophageal varices were examined endoscopically at six-month intervals, and brushing samples were taken. The ages of the patients ranged between 35 and 81 years, and all had portal hypertension due to cirrhosis; 10 patients with the same disease but without bleeding varices were also examined. The interval between sclerotherapy and the first cytologic follow-up examination averaged 34 months, while the interval to the last follow-up examination averaged 40 months. One patient was examined four times (1.4%), 10 three times (14.7%), 39 twice (57.4%) and 18 only once (26.5%). Two cases were interpreted as nuclear hyperplasia associated with inflammation and were found to have regressed at a subsequent examination; all the other cases were reported as negative although sometimes associated with inflammation. None of the controls showed any abnormalities. While the occurrence of esophageal cancer after sclerotherapy might be associated with other risk factors, such as alcohol intake and smoking, esophageal brushing cytology can successfully monitor these patients and detect early stages of neoplasia.

Adult↗
PubMedDate not supplied

Identification of granulocyte-macrophage colony stimulating factor receptor mRNA by non-isotopic in situ hybridization in bone marrow biopsies.

BACKGROUND: In the last few years many studies have been published on GM-CSF receptors, focusing on molecular structure, function and distribution. Nevertheless, protocols for detecting GM-CSF receptors on formalin-fixed paraffin-embedded histological sections, to our knowledge, have not been described. METHODS: A method based on non-isotopic in situ hybridization (ISH) using a 21-base antisense DNA oligoprobe whose 3'-end was labeled with digoxigenin 11-dUTP was devised. The probe was applied on 20 routinely processed bone marrow trephine biopsies which were considered as positive controls. RESULTS: The hybridization signal was seen in myeloid cells, erythroid progenitors and rare megakaryocytes. CONCLUSIONS: Non-isotopic ISH represents an alternative to current methodologies for the assessment of GM-CSF receptor expression; since it is suitable for routinely processed samples, it can be regarded as a helpful tool for diagnostic determination of GM-CSF receptors in tumors from patients receiving GM-CSF and for retrospective studies on archival material.

Base Sequence↗