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Biomedical subjects

R E Banks

Publications and source records attributed to R E Banks.

62 records · Page 4Linked to original sources
PubMed1989

Antigen delivery to gut associated lymphoid tissue of rabbits.

A rabbit model was developed so that antigens, microbes or potential mucosal vaccines could be delivered in multiple doses directly to the luminal surface of the gut associated lymphoid tissue (GALT) located in the cecal appendix. A polyethylene catheter was implanted surgically into the tip of the appendix. It exited the body wall and progressed subcutaneously around the thorax to the dorsocervical region where it was attached to a subcutaneous obturator. The catheters were flushed every day with sterile saline. To assess patency of the system, radiographic contrast media was injected and the animals were radiographed. Rabbits were necropsied at 10, 30 and 60 days and tissues evaluated by histological, immunohistochemical and electron microscopic means. The microbial flora was evaluated also. All tissues examined were free of significant abnormalities. The advantage of this delivery system is its ability to provide multiple doses of immunogenic agents directly to a physiologically normal appendix over an extended period.

Animals↗
PubMed1988

A paracostal approach for splenectomy in the sheep.

A modified left paracostal approach was developed to splenectomize sheep. This approach was found to be superior to the described approaches for removal of the spleen by rib resection. Since the diaphragm is in close approximation to the last rib, resection of the last rib can result in entry of the thoracic cavity, which will complicate the surgical procedure. Furthermore, rib resection necessitates the use of analgesics, due to the pain commonly associated with any orthopedic procedure, and thereby extends postoperative recovery time. The paracostal abdominal approach provides excellent exposure, ease of manipulation, reduced anesthetic time, and fewer related problems such as postoperative pain control and thoracic evacuation. A major benefit of the paracostal approach is the minimal need for analgesic support as evidenced by the animals' rapid return to normal function. This bypasses the need for extended recovery, as required in the rib resection approach, and allows earlier utilization of the animal for research.

Animals↗
PubMed1988

Ameloblastic odontoma in a cynomolgus monkey (Macaca fascicularis).

An ameloblastic odontoma occurred in an adult cynomolgus monkey (Macaca fascicularis). The neoplasm involved the upper left maxilla as a disfiguring, fleshy growth. The tissue and cellular changes were consistent with an ameloblastic odontoma which is rare in man, nonhuman primates (NHP), and other vertebrate animals. The monkey was one of 50 adult, single-caged housed cynomolgus monkeys. No additional clinical signs of disease were present. Hematology and serum chemistries were within normal limits. There have been three reports in the literature of ameloblastic odontomas in NHP; however, this is the first reported case of an ameloblastic odontoma in a cynomolgus monkey.

Animals↗
PubMed1988

Surgical excision of an ameloblastic odontoma in a cynomolgus monkey (Macaca fascicularis).

A surgical approach used for removal of maxillary tumors in humans was modified to excise a maxillary tumor in a cynomolgus monkey (Macaca fascicularis). This is an effective technique for excising tumors, recovering fractured canine teeth, or other health problems of the maxillary region in Macaca sp. The described approach limits facial disfigurement and enhances the probability of return to required function for future research utilization. This approach minimizes the extensive degree of post-operative intensive care.

Animals↗
PubMedDate not supplied

The potential use of laser capture microdissection to selectively obtain distinct populations of cells for proteomic analysis--preliminary findings.

Proteomics-based studies offer a powerful complementary approach to DNA/RNA-based investigations and are now being applied to investigate aspects of many diseases including cancer. However, the heterogeneous nature of tissue samples often makes interpretation difficult. We have undertaken a study into the potential use of a novel laser capture microdissection (LCM) system to isolate cells of interest for subsequent proteomic analysis. Retrieval of selected cells is achieved by activation of a transfer film placed in contact with a tissue section, by a laser beam (30 or 60 microm diameter) which is focused on a selected area of tissue using an inverted microscope. The precise area of film targeted by the laser bonds to the tissue beneath it and these cells are then lifted free of surrounding tissue. Although the technique has been shown to be readily compatible with subsequent analysis of nucleic acids, little information is yet available regarding the application of protein-based analyses to the captured tissue. We report here preliminary data regarding the potential use of the LCM system in combination with two-dimensional electrophoresis to examine protein profiles of selected tissue areas. Electrophoretic profiles of proteins from normal and malignant renal tissue samples showed little change following LCM, nine selected proteins showed identical mass spectrometric sequencing profiles, and two selected proteins retained antigenicity. Dissection of epithelial tissue from a sample of normal human cervix resulted in enrichment of some proteins compared with analysis of the whole tissue. LCM will be a valuable adjunct to proteomic studies although further detailed validation is necessary.

Amino Acid Sequence↗
PubMedDate not supplied

Cation transport in lymphoblastoid cell lines established from bipolar manic-depressive patients.

Lymphoblastoid cell lines established from patients suffering from bipolar manic depression have been used to study the possible involvement of cation transport in the aetiology of this illness. No significant difference was found in the K+ fluxes mediated by the ouabain-sensitive sodium pump, the diuretic-sensitive cotransport system and the passive leak pathway of cell lines established from either control or bipolar subjects. The mean value for the specific binding of 3H-ouabain (sodium pump site number) was significantly higher in the bipolar group (approximately 30%) than in the control group.

Adult↗
PubMedDate not supplied

Analysis of vocal fold function in the miniswine model.

Correlation of the vocal fold vibratory pattern with the postoperative surgical wound should demonstrate to the laryngologist the critical depth of injury at which the functional voice would be adversely affected. An animal model was selected to study these changes. Twelve adult miniswine were operated on in this prospective study. The right true vocal fold was surgically altered; the left true vocal fold was the control. An interval of 6 weeks was allowed for healing, and then changes in vocal fold function were studied by stroboscopy, photography, voice recording, and electroglottography. Laryngectomy provided tissue for histopathological correlation. The results showed that injury at the junction of the lamina propria and vocalis muscle consistently caused significant vibratory dysfunction resulting in poor phonation.

Animals↗