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Biomedical subjects

R E Billingham

Publications and source records attributed to R E Billingham.

At least 19 recordsLinked to original sources

Parental divorce during childhood and use of violence in dating relationships.

355 women and 173 men university students between the ages of 17 and 23 yr. completed a questionnaire which included Straus's Conflict Tactic Scales, and from which whether the respondents had experienced the divorce of their parents could be assessed. Two hypotheses were tested. First, individuals from divorced families would be more likely to report that violence existed in their current relationship than would individuals from intact families. Second, in those relationships which included violence, individuals from divorced families would report higher levels of violence than would individuals from intact families. Analyses indicated that individuals from divorced families were no more likely to report themselves as experiencing violence in their current relationship than were their cohorts from intact families. However, when the conflict tactic scores for men from divorced, men from intact, women from divorced, and women from intact families were compared, differences were found. Men from divorced families reported levels of violence for themselves that approached significance. Also, men from divorced families reported that their partners' verbal aggression approached significance and that their partners' use of violent behavior reached significance when compared to those of the other groups. These results indicate that having experienced a divorce in one's family may have a greater effect on later relationships for men than for women.

Adolescent

Relationship between egocentrism, alcohol consumption and reasons given for drinking.

University students, 86 men and 141 women, completed a questionnaire from which their level of egocentrism, amount of alcohol consumed, and number of reasons for drinking could be estimated. Analysis supported earlier findings that men consume significantly more alcohol than do women. Both men and women who were rated as having high egocentrism scores gave significantly more reasons for drinking than did the women scoring low on egocentrism. Also, there was a significant correlation between egocentrism and reasons for drinking but not between egocentrism and amount consumed. The correlation was higher between the number of reasons given for drinking and the amount of alcohol consumed. Since men scoring high on egocentrism report the highest consumption, while high-scoring women report the lowest consumption, it is concluded that egocentrism plays an indirect role in the consumption of alcohol and the role it plays is different for men and women.

Adult

The effect of chronological and theoretical birth order on sexual attitudes and behaviors.

A survey designed to evaluate the impact that both chronological and theoretical birth order may have on sexual attitudes and behaviors was completed by 441 respondents (221 male, 220 female). The attitudes and behaviors investigated were aggressive sexual behavior, social relationship attitudes, emotional relationship attitudes, total heterosexual behavior, and total orgasmic behavior. The data revealed no difference between male and female in terms of total sexual behavior. However, males had significantly higher sexual aggression, social attitudes, emotional attitudes, and total orgasm scores. These results suggest that while there may be equity between the sexes in terms of heterosexual behavior, there are still significant differences between the sexes in terms of specific behaviors and attitudes. No differences were found on any of the scales based on either chronological or theoretical birth order. These results suggest that sexual behavior and attitudes may be influenced to a greater degree by biological and cohort factors than by sibling position.

Adolescent

Courtship violence.

Historically, the courtship relationship is thought to be one of peace and tranquility. However, recent research has demonstrated that many dating relationships include violence as a means to resolve conflicts. While differences have been found in the prevalence of violence between high school and college students, both groups report similar locations, causes, and consequences for the behavior. Peers are cited most often as confidants for students who have experienced the violence. Unfortunately, almost 25% of those who experience violence report it to no one. This paper also describes means school health personnel can use to discuss courtship violence with students. By demonstrating awareness of and sensitivity to the problem, school health personnel may be able to counter use of violence in dating relationships.

Adolescent

Sexual orientation and boyhood gender conformity: development of the Boyhood Gender Conformity Scale (BGCS)

Two hundred twenty-five [corrected] respondents (109 [corrected] heterosexuals and 116 [corrected] homosexuals) completed a survey containing a 20-item Boyhood Gender Conformity Scale (BGCS). This scale was largely composed of edited and abridged gender items from Part A of Freund et al.'s Feminine Gender Identity Scale (FGIS-A) and Whitam's "childhood indicators." The combined scale was developed in an attempt to obtain a reliable, valid, and potent discriminating instrument for accurately classifying adult male respondents for sexual orientation on the basis of their reported boyhood gender conformity or nonconforming behavior and identity. In addition, 33% of these respondents were administered the original FGIS-A and Whitam inventory during a 2-week test-retest analysis conducted to determine the validity and reliability of the new instrument. All the original items significantly discriminated between heterosexual and homosexual respondents. From these a 13-item function and a 5-item function proved to be the most powerful discriminators between the two groups. Significant correlations between each of the three scales and a very high test-retest correlation coefficient supported the reliability and validity assumption for the BGCS. The conclusion was made that the five-item function (playing with boys, preferring [corrected] boys' games, imagining self as sports figure, reading adventure and sports stories, considered a "sissy") was the most potent and parsimonious discriminator among adult males for sexual orientation. It was similarly noted that the absence of masculine behaviors and traits appeared to be a more powerful predictor of later homosexual orientation than the traditionally feminine or cross-sexed traits and behaviors.

Adolescent

Activation of rabbit keratinocyte fibronectin receptor function in vivo during wound healing.

Freshly isolated rabbit keratinocytes expressed low fibronectin (pFN) receptor function as shown by their poor ability to attach and spread on pFN-coated substrata or to bind and ingest pFN-coated beads. Following in vitro culture of these cells, however, pFN receptor function was activated. The cultured cells appeared to be normal, based on their ability to reepithelialize rapidly full-thickness cutaneous wound beds. Freshly isolated keratinocytes that had low pFN receptor function were autotransplanted onto full-thickness wound beds. Two days after transplantation, keratinocytes recovered from these wounds were observed to express increased pFN receptor function. This activity was maximal in keratinocytes isolated 3 days after transplantation and declined in keratinocytes isolated at later times. By 10 days after transplantation, the transplanted cells had formed a multilayered hyperplastic epidermis and reconstituted their laminin and type IV collagen-containing basement membrane. It is proposed that initiation of pFN receptor function in keratinocytes is a crucial mechanism necessary for them to attach to and migrate through the pFN-rich wound bed comprised of granulation tissue. After reepithelialization is complete, and the basement membrane re-forms, pFN receptor function declines markedly because it is no longer essential to the cells.

Animals

Concerning the immunology of the uterus.

As a mucosal organ, the uterus is unique in being subject to exposure to three types of antigen: chronically to micro-organisms, episodically to sperm bearing both auto- and alloantigens as well as soluble antigens in seminal plasma, and to conceptuses--nature's allografts--expressing transplantation antigens. That the uterus is effective in handling pathogens is evidenced by the rarity of infections. Local macrophages, immunocytes, and a secretory immune system, most evident in the cervix, probably underlie this attribute. Mammals' reproductive success hints that the uterus is immunologically discriminating toward the nonharmful but biologically important antigens of classes 2 and 3. Why sperm are not normally destroyed immunologically in the female prior to fertilization and why conceptuses are not rejected are central unresolved questions in reproductive immunology. Undoubtedly, important immunoregulatory principles are operating in this remarkable organ and its decidua. Better to understand antigenic exposure in the rat uterus, we have reinvestigated its lymphatic drainage using labeled cell injection and microanatomical procedures. Despite abundant lymphatics in the myometrium, cells placed in the uterine lumen did not readily gain access to the lymphatic system. There was a paucity of lymphatics in the endometrium, but Ia-positive dendritic cells were abundant. Soluble antigen exposure via the uterus failed to evoke a primary antibody response yet did prime the host for a secondary response. This restriction of systemic antigen presentation from the uterus, in conjunction with both cellular and noncellular immunoregulatory principles acting locally, may be important to ensure maternal hyporesponsiveness to paternal alloantigens.

Animals

Recipient treatment to overcome the allograft reaction, with special reference to nature's own solution.

The 6th decade of this century was particularly important for transplantation immunology. The universality of allograft rejection by normal hosts had won general acceptance and, experimentally, several means of abrogating host reactivity to allografts were discovered. These included sub-lethal whole body irradiation, administration of certain corticosteroid hormones and inoculation of very young animals with living cellular inocula from the future graft donor--i.e., classic, neonatal tolerance. The latter was particularly important since it indicated the feasibility of a specific, permanent solution to the clinical allograft problem. Radiation and drug-induced tolerance in adult subjects came along and chemical immunosuppressants, which led to successful clinical use of azathioprine. The important rediscovery of ALS pointed towards the development and clinical application of monoclonal antibodies many years later. With the development of immunogenetics and transplantation biology came recognition that the conceptus is a highly successful allograft, raising the question of how it is able to withstand rejection by its immunocompetent mother for the duration of pregnancy. Hopefully, knowledge of the principle(s) involved when they are finally elucidated will be applicable to clinical allograft recipients. Although functional hypoantigenicity of the syncytial trophoblast probably plays a major role in protecting the allogeneic conceptus, a strong case now exists that local, cell-based, immunosuppressive and immunoprotective activity within the placenta and decidua, mediated by suppressor and other cells, is important.

Animals

Premarital contraceptive use: a discriminant analysis approach.

The ability of seven independent variables to predict accurately, from a sample of unmarried, sexually active students, which ones use reliable contraceptives and which ones use unreliable contraceptives, was investigated. Using discriminant analysis, the seven independent variables were age individuals started engaging in coitus, frequency of coitus, frequency of dating, length of time sex partners knew each other, number of sex partners, having anticipated coitus to occur, and the number of close friends who were thought to use birth control. The results indicated that these variables were accurate in predicting which students were users of contraceptives, with more than 80% of males and females being correctly classified. The number of close friends thought to use contraceptives was the most influential variable for both sexes, followed by length of time the partners knew each other.

Adult

Maternal--fetal immune interactions and the maintenance of major histocompatibility complex polymorphism in the rat.

In several different strain combinations of rats, an R2 backcross was used to assess the relative fitness of progeny MHC-incompatible with their mothers as compared to that of MHC-compatible progeny. None of the various treatments used to alter maternal immunoregulatory processes, namely splenectomy, active or adoptive immunization, or the induction of transplantation tolerance, was able to perturb consistently the population distribution of RT1 antigens. Though the extreme variability of RT1 heterozygote excess remains poorly understood, studies of parity-associated changes in the population distribution of RT1 heterozygotes: RT1 homozygotes nonetheless do support the hypothesis that the spleen is important to the immunoregulatory control of these phenomena.

Animals

Immune privilege in the testis. II. Evaluation of potential local factors.

The significance of several local factors in prolonging allograft survival in the rat testis has been investigated. The lower temperature of this organ was shown to have no effect, because parathyroid gland allografts implanted s.c. in the ear, which is similarly hypothermic, were rejected as promptly as in conventional sites. Conversely, testes secured in the abdominal cavity afforded privilege to grafts just as well as normal testes. With indirect immunofluorescence using a monoclonal antibody, it was shown that the testis' interstitial tissue is well-endowed with cells bearing class II histocompatibility antigens, a prerequisite for antigen-processing capability. When intratesticular allograft recipients were pretreated with estrogen to suppress Leydig cell synthesis of testosterone, most grafts were rejected promptly, suggesting that local steroid secretion is important in testicular immune privilege. Both testes and s.c. sites supported inflammatory reactions to cotton pledgets, indicating that there is no interference with the nonspecific inflammatory process necessary for graft rejection, but more likely with antigen processing itself.

Animals

H-Y antigen and immunity: a means for controlling the secondary sex ratio?

C57BL/6J female mice were splenectomized and then sensitized to the H-Y antigen by means of grafts of H-Y incompatible skin and injections of spleen cells from syngeneic males. When subsequently mated to syngeneic males the secondary sex ratio of progeny born was significantly greater than that of controls. However, the effect was manifest only among the progeny of later litters (i.e., 3, 4, 5) suggesting that repeated exposure to the H-Y antigen during the course of pregnancy was essential to bring about demonstrable immunoregulatory changes.

Animals

Reconsideration of the lymphatic drainage of the rat testis.

An extensive investigation of the lymphatic drainage from the rat testis was carried out in an effort to detect any characteristics which might be important in the immunological privilege the testis extends to allografts. Three different methods revealed a consistent and very efficient lymphatic drainage involving primarily the iliac and renal lymph nodes and to a lesser extent the external lumbar, para-aortic, and posterior gastric nodes. Within minutes, dye or labeled cells injected into the testis could be found within regional nodes. Node hypertrophy was induced by injecting the testes of F1 hybrids with parental strain lymphoid cells (a regional graft-versus-host reaction). Besides indicating that efficient filtration occurs in these nodes, this also established that they are a hospitable environment for cellular immune reactions. Similarly, the capacity of allogeneic cells injected into the testis to induce production of humoral alloantibodies by recipients confirms that exposure to antigens via this route does not in itself suppress immune responsiveness.

Animals

Immune privilege in the testis. I. Basic parameters of allograft survival.

The fate of solid tissue allografts--skin or parathyroid glands--implanted in the interstitial tissue of the testis was investigated using inbred rats. The results affirm that the testis is an immunologically privileged site despite its efficient lymphatic drainage. Skin allografts survived at least several days longer than orthotopic grafts of similar size, whether major histocompatibility complex (MHC)-compatible or MHC-incompatible, and failed to induce an alloantibody response in most recipients or to prime for secondary antibody responses on rechallenge. Further assessment employed parathyroid grafts that allowed appraisal of their function and its duration, by monitoring serum calcium levels. Most intratesticular MHC-incompatible parathyroids survived for at least twice as long as control grafts in nonprivileged sites, with a median survival time (MST) of 41 days--and one-third of the grafts functioned at 100 days. MHC-compatible grafts fared even better (MST of 60 days), some surviving more than 400 days. Most F1 hybrid grafts survived virtually indefinitely. Splenectomy 5-23 days prior to implantation had a beneficial rather than a detrimental effect on the privilege afforded intratesticular parathyroid allografts. Allograft rejection was accompanied by antibody production in only one-half the animals. Grafts that had undergone functional rejection at the time of recovery usually had an intense mononuclear cell infiltrate, and long-term surviving grafts displayed varying degrees of cellular infiltration among cords of healthy, functional chief cells. Accepted parathyroids were destroyed by active immunization of the host but were unaffected by passively administered alloantibodies. The possible mechanisms controlling graft rejection in this unique privileged site are discussed.

Animals

The H-Y antigen and its role in natural transplantation.

A concise overview of the transplantation biology of the H-Y antigen is presented with particular reference to: its prototypic behavior as a weak transplantation antigen; the facility with which mice of certain inbred strains can be rendered tolerant of H-Y incompatible skin grafts; its capacity to instigate graft-versus-host reactions; its significance in clinical transplantation; and finally, H-2 control of anti-H-Y immune responses. The role of the H-Y antigen in natural transplantation, i.e. pregnancy, is then reviewed. Evidence is presented to support the hypothesis that in certain cases maternal immune responses directed specifically to the H-Y antigen can exert selective pressures on male zygotes, producing deviant sex ratios in certain experimental and clinical situations.

Animals