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Biomedical subjects

R E Callard

Publications and source records attributed to R E Callard.

82 records · Page 5Linked to original sources
PubMed1981

Specific in vitro antibody response to varicella zoster.

Peripheral blood mononuclear cells from normal donors were stimulated in vitro by varicella zoster (VZ) antigen and antibody measured by an ELISA technique. Antibody was produced in 14 of 19 individuals. This antibody was specific for VZ with no binding to herpes simplex virus (HSV), even when the mononuclear cells were taken from individuals who had circulating antibody to VZ and HSV. The failure of antibody production in mononuclear cell cultures from some normals, all of whom had serum antibody, was analysed using reciprocal combinations of E+ and E- cells from producers and non-producers. E+ cells from producers could help E- producer cells make antibody, but E+ cells from non-producers could not help E- cells from non-producers to do so. This result suggests a deficiency in circulating E- cells in non-producers. Four of four patients with untreated Hodgkin's disease were non-producers using blood mononuclear cell cultures, but their spleen cells produced antibody readily. This suggests that failure of in vitro antibody production in immune individuals may be due to sequestration of responsive B cells in spleen and other sites. This technique allows further study of the mechanisms of immunity to VZ in normal and immune-compromised individuals.

Adult↗
PubMed1981

Unusual phenotype and function of an expanded subpopulation of T cells in patients with haemopoietic disorders.

We have studied two patients, one with red cell aplasia and the other with neutropenia. Both showed lymphocytosis. In both cases, 90-100% of E rosetting cells were T cells as defined by the monoclonal antibodies UCHT1 and OKT3. The majority of these cells also carried the OKT8 suppressor/cytotoxic marker and were HLA-DR- and Fc gamma R-positive. In spite of the similarity of this phenotype to that reported for suppressor cells, these cells failed to suppress pokeweed mitogen-induced polyclonal Ig synthesis. Cells from both patients also failed to respond significantly to Con A and PHA. They were, however, unable to suppress the Con A responses of normal donors although cells from one patient were able to suppress completely a normal PHA response. These results demonstrate the existence of a genuine subset of T cells with Fc gamma receptors but suggest that not all such cells have typical suppressor function.

Agranulocytosis↗
PubMed1979

Specific in vitro antibody response to influenza virus by human blood lymphocytes.

Advances in understanding of human immune responses depend, for obvious reasons, on the use of in vitro techniques for culture of peripheral blood lymphocytes (PBL). We report here that specific antibody responses to influenza virus can readily be obtained using simple, reproducible methods. The system will be useful for the analysis of the cellular requirements for antibody production, the genetics of immune responsiveness and in clinical studies of immunosuppressed and immunodeficient patients.

Antibodies, Viral↗