Energy metabolism of the renal medulla.
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Biomedical subjects
Publications and source records attributed to R E DAVIES.
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Potassium accumulation occurred in leached slices of the inner medulla of the dog kidney incubated under anaerobic conditions at 37 degrees C with glucose as substrate, but was blocked by inhibitors of glycolysis. Aerobically, only slight accumulation occurred with succinate as substrate at 37 degrees C, and this was blocked by dinitrophenol. These findings were in contrast to those obtained with kidney cortex slices under the same conditions.
Kidney cortex slices incubated in vitro at 0 degrees C. accumulate radiosulfate from the incubation medium. This process differs from the previously described uptake of radiosulfate by renal tissue incubated at 38 degrees C., for instance, in the lesser sensitivity of the uptake at 0 degrees C. to differential effects of Na(+) as compared with K(+) ions, and of sucrose as compared with glucose. Phlorizin inhibits radiosulfate accumulation at 0 degrees C., whereas it enhances the uptake at 38 degrees C. Effects of the cations K(+) and Na(+) and of phlorizin at temperatures intermediate between 0 degrees and 38 degrees C. have been studied. Parallels have been noted between the accumulative processes for radiosulfate of kidney slices maintained at 0 degrees C. and of mitochondria isolated from rat liver and kidney cortex. These similarities may be attributed to an important role of radiosulfate uptake by mitochondria in slice accumulation of radiosulfate in the cold.
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