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R E Domen

Publications and source records attributed to R E Domen.

16 recordsLinked to original sources
PubMed1990

Zeta potential and vancomycin-red blood cell interactions.

Vancomycin hydrochloride is a polycationic antibiotic that we have previously reported to cause spontaneous aggregation of red blood cells (RBCs) in vitro at concentrations of more than 3.0 mg/mL, and false-positive direct antiglobulin tests at concentrations of 2.0 and 2.5 mg/mL. These latter reactions were abolished when the RBCs were pretreated with the proteolytic enzyme ficin suggesting an interaction with sialoglycoproteins. In this study we show that prior addition of vancomycin did not block binding of antibodies to several blood group antigens, nor does vancomycin precipitate in an Ouchterlony assay with any of several sialic acid moieties. Finally, we show that vancomycin possesses fluorescent properties but fails to be detected on the RBC surface by flow cytometry. These data indicate that vancomycin may be acting through its polycationic properties. Our findings do not support protein binding of vancomycin to the RBC surface, but underline the importance of the zeta potential in RBC serologic reactions.

Erythrocyte Membrane↗
PubMed1989

Vancomycin-induced red cell aggregation.

Vancomycin, an antibiotic similar in structure to ristocetin, is used to treat staphylococcal infections. However, vancomycin-induced hemagglutination complicated red cell (RBC) serologic testing in the blood bank. At concentrations greater than 3.0 mg per ml, vancomycin caused spontaneous macroscopic red cell (RBC) aggregation; concentrations of 2.0 and 2.5 mg per ml were associated with weakly positive aggregation with anti-IgG and polyspecific antiglobulin reagents negative with anti-complement; and concentrations less than 1.5 mg per ml had no apparent effect. Ficin-treated RBCs demonstrated negative reactions with the antiglobulin reagents. Vancomycin-induced aggregation was reversed partially with 0.2 M trisodium citrate, and supernatant transfer studies showed that normal RBCs retained a significantly (p less than 0.025) greater percentage of vancomycin than did ficin-treated RBCs. Vancomycin causes the aggregation of RBCs, which can be a source of confusion in the blood bank. The mechanism(s) through which vancomycin enhances aggregation may be related to its polycationic properties and to its direct protein binding to the RBC membrane, although other nonimmunologic mechanisms may be operative.

Citrates↗
PubMed1988

Red cell alloimmunization in chronic renal failure patients undergoing hemodialysis.

Posttransfusion alloimmunization to red cell antigens was studied in a group of 98 chronic renal disease patients treated solely with hemodialysis. Clinically significant red cell antibodies were formed in only 6.1% of transfused patients, and only 1 patient formed more than one antibody. We do not recommend extended red cell phenotyping, beyond ABO and Rh(D), in patients with end-stage renal disease who may require transfusion.

Adult↗
PubMed1986

Efficacy of performing red-cell antibody elutions in patients with a positive direct antiglobulin test.

Red blood cell antibody elutions are often routinely performed whenever a positive direct antiglobulin test (DAT) is encountered. To evaluate the efficacy of performing routine red-cell elutions we reviewed our antibody elution data. Of 122 eluates, 83 were negative, 35 were warm panagglutinins, 2 were felt to be transfusion-induced alloantibodies, 1 was passively acquired anti-A, and 1 was inconclusive. One of the eluted alloantibodies was not demonstrable in the serum. Thus, only 1 (0.8%) of the eluates provided important information not readily available through serum testing alone. We conclude that extensive serologic evaluation of a positive DAT should be reserved for those patients who have been recently transfused or are suspected of having immune hemolysis.

Agglutinins↗
PubMed1986

Coagulation and immunology.

One of the most vital functions of the vertebrates is to maintain hemostasis through coagulation and fibrinolysis. This is achieved by the interaction of vascular walls with blood components such as cells and proteins (coagulation factors, immunoglobulins, and complement). Deficiency of coagulation factors results in bleeding disorders. The immune system, in response to foreign proteins or defective self proteins, produces antibodies triggering an Ag-Ab interaction with the related host of different reactions including abnormal coagulation leading to hemorrhagic diathesis.

Antigen-Antibody Complex↗
PubMed1979

Granular cell myoblastoma of the esophagus after irradiation for carcinoma.

Granular cell myoblastoma is an uncommon, usually benign tumor. Only 20 cases are reported in the esophagus. We describe a 65-year-old woman who developed a granular cell myoblastoma of the postericoid esophagus in the area of a squamous cell carcinoma successfully treated with irradiation. To our knowledge, this is the 21st reported case, and the only case occurring in the esophagus after irradiation for primary squamous cell carcinoma.

Aged↗