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Biomedical subjects

R E Jordon

Publications and source records attributed to R E Jordon.

At least 109 records · Page 6Linked to original sources
PubMed1975

The complement system in bullous pemphigoid. III. Fixation of C1q and C4 by pemphigoid antibody.

By in vitro complement immunofluorescent staining methods, 46 serum samples from bullous pemphigoid patients with titers of antibasement-membrane-zone antibodies of 320 or greater were tested for their ability to fix C1q, C4, and C3. All three staining reactions were positive with 23 of the samples; 21 samples yielded negative reactions. With one sample, the C1q and C3 staining reactions were positive but the C4 reaction was negative; one other sample gave a positive C3 staining reaction only. Absorption of anti-C1q, anti-C4, and anti-C3 with C1q, C4, and C3, respectively, blocked the specific in vitro complement staining. Therefore, bullous pemphigoid antibodies of the IgG type appear to be capable of activating the "classical" complement pathway.

Autoantibodies↗
PubMed1975

[Chemotactic activity in the fluid of bullous pemphigoid blisters].

By a modified Boyden technique, chemotactic activity was present in bullous pemphigoid blister fluids but was also present in the corresponding sera. Heat inactivation (56 degrees C for 30 minutes) only partially reduced the blister fluid chemotactic activity, but almost completely inhibited the activity present in pemphigoid sera. Control blister fluids exhibited some chemotactic activity, but in contrast to pemphigoid blister fluids, this activity was almost entirely abolished by heat inactivation. The chemotactic activity remaining in heat inactivated pemphigoid blister fluid was inhibited by N-CBZ-alpha-glutamyl-L-tyrosine and by antiserum to C5 but not with antiserum to C3. Our studies suggest that complement-dependent chemotactic activity is present in bullous pemphigoid blister fluids, findings which further implicate complement activation in the pathogenesis of this disease.

Blister↗
PubMed1973

The complement system in bullous pemphigoid. I. Complement and component levels in sera and blister fluids.

Compared with other serum and blister fluid proteins, total hemolytic complement was reduced in the blister fluid of six serologically positive bullous pemphigold patients while four serologically negative cases had blister fluid complement levels closely approaching the serum levels. Except for pemphigus vulgaris blisters. blister fluids from most patients with other bullous diseases and experimentally induced blisters had blister fluid complement levels more closely approaching the serum levels. With the exception of the two terminal components. C8 and C9, individual components of the complement sequence were also reduced in the blister fluids of the six bullous pemphigold patients with circulating basement membrane zone antibodies. On the other hand, transferrin and IgG levels of these same six serologically positive blister fluids closely approached the corresponding serum levels. Conversion of C3 proactivator was also demonstrable in the serologically positive bullous pemphigoid blister fluids, but not in the corresponding sera. Our studies, therefore, are suggestive of local activation of the complement sequence, by both the classical and alternate pathways, in blisters of serologically positive bullous pemphigold patients.

Basement Membrane↗