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R E REEVES

Publications and source records attributed to R E REEVES.

18 recordsLinked to original sources
PubMed1963

PANTETHINE-REQUIRING BACTEROIDES.

Reeves, Richard E. (Louisiana State University School of Medicine, New Orleans). Pantethine-requiring Bacteroides. J. Bacteriol. 85:1197-1201. 1963.-Growth of a culture of gram-negative streptobacilli, provisionally designated Bacteroides symbiosus, required a preformed source of pantetheine (pantethine or coenzyme A). Five types of organisms were isolated from the parent culture, and all exhibited a pantethine requirement. Pantothenate neither replaced nor spared the pantethine requirement of these organisms. Of the five isolated bacterial types, three were characterized by producing low, medium, and high optical densities, respectively, in a thiomalate medium. A fourth type was distinguished by cells which would not pack upon centrifugation, and a fifth by its high content of stored carbohydrate. Two of the five types seem well-suited to the growth of Entamoeba histolytica in the MS-F ameba-culture system. In addition to pantethine, these organisms required biotin, folic acid, pyridoxine, thiamine, and choline. A minimal defined medium was elaborated for one of the isolated bacterial types. B. symbiosus ATCC 12829 is proposed as a test organism for pantetheine in the presence of pantothenate.

Bacteroides↗
PubMed1962

Studies on the carbohydrate metabolism of a gram-negative anaerobe (Bacteroides symbiosus) used in the culture of Entamoeba histolytica.

Bragg, P. D. (Louisiana State University, New Orleans) and R. E. Reeves. Studies on the carbohydrate metabolism of a gram-negative anaerobe (Bacteroides symbiosus) used in the culture of Entamoeba histolytica. J. Bacteriol. 83:76-84. 1962-Resting cells of Bacteroides symbiosus have been shown to utilize glucose and several other monosaccharides. The fermentation of the sugars is mediated by demonstrable kinases except in the case of mannitol. The main end products of metabolism of glucose are CO(2), H(2), ethanol, and acetic, butyric, succinic, and lactic acids. Changes in the thiol used in the growth media produce different enzyme complements in the cells. Thus, cells grown with cysteine as the thiol are unable to metabolize glucosamine, whereas those grown with thiomalate rapidly degrade the amino sugar. The results of the enzyme assay and the results from experiments with C(14)-labelled glucose suggest that glucose is metabolized by resting cells mainly by the Embden-Myerhof pathway.

Bacteroides↗