Biosynthesis of 6-beta-galactinol by beta-galactosidase from rat mammary gland and E. coli.
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Biomedical subjects
Publications and source records attributed to R E Ray.
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A disaccharide was isolated from rat mammary tissue and determined to be 6-O-beta-galactopyranosyl myo-inositol (6-beta-galactinol) on the basis of combined gas-liquid chromatography-mass spectrometry of the permethylated and peracetylated derivatives as well as previously reported chemical and enzymatic evidence. 6-beta-Galactinol, also found in rat milk, increased during lactation, and on the 18th day represented approximately 17% of the total non-lipid neutral myo-inositol. The sugar was absent in all other rat tissues examined, suggesting a unique association with the process of lactation. This novel galactoside of one human subject on the basis of paper and gas-liquid chromatography.
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