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Biomedical subjects

R E Toya

Publications and source records attributed to R E Toya.

15 recordsLinked to original sources
PubMed1977

Attempts to purify hemopoietic stem cell enrichment in bone marrow by use of glass wool filtration.

This study is about enrichment of hemopoietic stem cell concentration in mouse bone marrow by use of a glass wool filtration technique. Maximum stem cell concentration was accomplished when filtration conditions were as follows: (1) support medium supplemented to 15% with fresh serum from syngeneic donors; (2) no incubation time allowed for cells on the columns; and (3) an operational temperature of 24 degrees C. With these conditions, stem cell concentrations were increased 5,4-fold relative to the concentration in untreated marrow.

Animals↗
PubMed1975

Specific and nonspecific stimulation of resistance to the growth and metastasis of the line 1 lung carcinoma.

The effects of specific dead tumor cell immunization and nonspecific immunostimulation with Corynebacterium parvum on the s.c. growth of the line 1 carcinoma in syngeneic BALB/c mice have been studied. Injection of heavily irradiated line ) carcinoma cells did not inhibit the transplantability or growth of the line 1 carcinoma, and in certain cases these treatments actually prolonged the period of rapid growth. This latter observation was traced to a mild inhibition of metastatic spread, which itself can slow the s.c. tumor growth. Treatment of the mice with 0.25 mg of C. parvum 7 days prior to transplant of the tumor had no effect on its growth by itself; but in combination with i.v.-injected tumor cells, which themselves had no effect on tumor growth, a 45% inhibition of tumor growth was induced. These data demonstrate that, in the weekly immunogenic line 1 carcinoma system, inhibition of s.c. transplants requires not only exposure to tumor antigens but also stimulation of the immunological reactivity.

Animals↗
PubMed1968

Oncogenicity by methyl methanesulfonate in male RF mice.

The incidences of lung tumors and thymic lymphomas were increased in young adult male RF mice receiving 30 milligrams of methyl meth anesulfonate per kilogram of body weight daily in the drinking water throughout life. Differences in oncogenicity between treatment with methyl methanesulfonate and with dimethylnitrosamine or diethylnitrosamine suggest a qualitative difference between the site (or sites) of alkylation by methyl methanesulfonate and by dimethylnitrosamine or diethylnitrosamine within the nucleic acids.

Alkylation↗