PubMed Health⌕ Search

Biomedical subjects

R Edelman

Publications and source records attributed to R Edelman.

89 records · Page 5Linked to original sources
PubMed1968

Specific role of each human leukocyte type in viral infections. II. Phytohemagglutinin-treated lymphocytes as host cells for vesicular stomatitis virus replication in vitro.

The mitogenic agent, phytohemagglutinin (PHA), added to human mixed leukocyte cultures and to lymphocyte cultures converted small lymphocytes into lymphoblasts and increased lymphocyte susceptibility to vesicular stomatitis virus (VSV). Maximum virus yields were 30- to 1,000-fold higher in PHA-treated than in control cultures. VSV replicated to peak titers before lymphocytes were morphologically transformed by PHA, and virus titers fell as lymphoblast destruction began. PHA neither induced significant VSV replication in polymorphonuclear leukocyte cultures, nor increased the large virus yields in monocyte cultures. The treatment of PHA with heat, digestive enzymes, rabbit anti-PHA serum and serial dilutions failed to dissociate that portion of the PHA extract responsible for the conversion of lymphocytes into virus-susceptible cells from those components responsible for leukoagglutination or lymphocyte transformation.

Culture Techniques↗
PubMed1967

Specific role of each human leukocyte type in viral infections. I. Monocyte as host cell for vesicular stomatitis virus replication in vitro.

Each major leukocyte type of the peripheral blood of healthy donors was studied in vitro for its ability to support vesicular stomatitis virus (VSV) replication. Purified cultures of each white blood cell type were prepared by the selective adsorption and elution of cells from silicone-treated glass beads. It was found that monocytes and macrophages (derived from the rapid transformation of monocytes in vitro) were the principal host cells for VSV replication. Interferon added to mixed leukocyte cultures, prior to virus inoculation, reduced virus yields and prevented destruction of macrophages. Cultures of small lymphocytes, containing no detectable monocytes or macrophages, produced amounts of virus equivalent to 1% of that produced in leukocyte cultures which contained 7% monocytes. Small lymphocytes did not undergo demonstrable cytopathic alterations in virus-infected cultures. VSV neither replicated nor produced cytopathic effects in polymorphonuclear leukocytes.

Culture Techniques↗
PubMed1966

Vesicular stomatitis virus replication in human leukocyte cultures: enhancement by phytohemagglutinin.

The replication of vesicular stomatitis virus in human leukocyte cultures shows that virus yields can be enhanced 6-to 180-fold by treating the leukocyte cultures with phytohemagglutinin prior to virus inoculation. Data suggest that a substance is produced in phytohemagglutinin-treated leukocyte cultures which is capable, on transfer to fresh leukocytes, of inducing blast cell formation and enhancing virus replication.

Adult↗
PubMedDate not supplied

Urinary neopterin in volunteers experimentally infected with Plasmodium falciparum.

To investigate the kinetics of monocyte/macrophage activation in falciparum malaria we determined urinary neopterin values serially in experimentally infected volunteers. Three subjects who had been immunized with irradiated sporozoites via mosquito bites served as controls. These individuals remained aparasitaemic, afebrile and without a rise in neopterin after challenge by infective mosquitoes. Four non-immune subjects developed Plasmodium falciparum parasitaemia, fever (3 of 4) and sharp rises in neopterin. Parasite densities reached 10-100 parasitized erythrocytes per microliter before elevations in temperature or neopterin levels were detected. Onset of fever preceded the rise in neopterin excretion by one day. Prompt chemotherapy was associated with the clearance of parasites from the blood and the return of temperature and neopterin levels to normal.

Biopterins↗
PubMedDate not supplied

Fibular regeneration.

Total regeneration of an osteotomized fibula is rarely reported in the literature. An extensive literature review failed to identify a publication addressing this specific topic. The following manuscript describes such an event where the fibula was used as a source of autogenous bone graft. A case report with long-term follow-up is also presented.

Bone Cysts↗
PubMedDate not supplied

Congenital absence of the tibial sesamoid: a report of two cases.

Congenital absence of the tibial sesamoid is a rare condition that has been infrequently reported in the podiatric literature. Two cases of congenital absence of the tibial sesamoid are presented with an associated hallux abducto valgus deformity. The role of a weakened medial flexor hallucis brevis tendon as a predisposing factor leading to a hallux abducto valgus deformity is discussed.

Adult↗
PubMedDate not supplied

A new technique for tenodesis of the tibialis posterior in the Kidner procedure.

When performing the Kidner procedure, the tibialis posterior tendon is advanced and reattached to the navicular. The authors present a new technique utilizing a cancellous screw and polyacetal resin washer to attach the tendon to the navicular. A case presentation and discussion of the procedure is presented.

Adult↗
PubMedDate not supplied

The Hohmann bunionectomy utilizing A-O screw fixation: a preliminary report.

A 14-month retrospective study was performed on 11 patients (15 feet) who had corrective bunion surgery utilizing a modified A-O Hohmann bunionectomy. The study involved radiographic evaluation, range of motion measurements, and a subjective questionnaire. The results of the study suggest that the procedure is very effective in correcting mild to moderate bunion deformities.

Adult↗