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Biomedical subjects

R Eggers

Publications and source records attributed to R Eggers.

10 recordsLinked to original sources
PubMed1990

Triple staining of brain tissue for neuronal classification.

The described triple staining (PAP, aldehydefuchsin, and cresyl violet) enables the distinction of different neuronal types with regard to their content of neurotransmitters or neuropeptides and their lipofuscin pigmentation respectively. The use of filters improves the contrast of immunohistochemical and lipofuscin staining, and facilitates the differentiation of neurons under the microscope. An additional important point is the advantage that perikaria can be measured in the usual morphometric manner.

Brain↗
PubMed1989

Is there a correlation between morphological and cytogenetic findings in placental tissue from early missed abortions?

A retrospective study of 200 missed abortions was performed to determine whether morphological criteria alone are sufficient to ascertain a chromosomal aetiology. Placental changes were classified into five morphological and four morphometric groups, according to the severity of alterations, and were then correlated with the cytogenetic data. The rate of chromosome anomalies was approximately 50% and was thus not significantly different within the groups II-V, but it was 80% in group I, which covered the most severe placental alterations, namely the partial hydatidiform moles. There was a high incidence of triploidies in group I, trisomies with obligatory early lethality in groups II and III, and X-monosomies in group III. Our findings do not support previous evidence regarding the specificity of certain villous alterations in association with chromosome aberrations. Indeed, they indicate that the placental villi may react similarly to chromosomal and non-chromosomal disturbances and that placental morphology depends on the severity and the temporal onset of the underlying disorder rather than on its type. With respect to chorionic villus samplings (CVS), this would mean that an abnormal villous structure may be suggestive for a chromosome anomaly but does not exclude a normal karyotype.

Abortion, Missed↗
PubMed1988

Increased systemic availability of albendazole when taken with a fatty meal.

We have studied the systemic availability of oral albendazole in 6 patients with echinococcosis either fasting or with breakfast. Albendazole sulphoxide, the pharmacologically active principle, was assayed by HPLC. Mean plasma concentrations and AUCs were 4.5 times higher when albendazole was given with breakfast than when administered in the fasting state. We conclude that therapy of echinococcosis with albendazole requires the drug to be taken with meals and that administration on an empty stomach might be more appropriate when intraluminal effects are desired, e.g. for intestinal parasites.

Adult↗
PubMed1988

Steady-state extrarenal sorbitol clearance as a measure of hepatic plasma flow.

Hepatic plasma flow was assessed with sorbitol (hepatic extraction = 0.96) at steady state. After infusion of 50 mg/min for 3 h, total and renal sorbitol clearances were calculated, and the extrarenal clearance was obtained by taking the difference between the two. In normal volunteers, the mean (+/- SD) extrarenal sorbitol clearance was 10.6 +/- 2.1 ml/min.kg. In patients with various liver diseases, it was correlated more closely to the fractional clearance of indocyanine green (r = 0.83, n = 57) than the galactose elimination capacity (r = 0.66, n = 55). Hepatic vein catheterization showed that the hepatic extraction of sorbitol was always much higher than the extraction of indocyanine green; there was no evidence for extrahepatic, extrarenal sorbitol elimination. On the basis of these findings, sorbitol is kinetically superior to indocyanine green and, although the noninvasively determined extrarenal sorbitol clearance at steady state may not be equal to total hepatic plasma flow, it may at least be regarded as a measure of parenchymal liver plasma flow.

Adult↗
PubMed1985

Thiamine deficiency and nervous system function disturbances.

Thiamine is important for oxidative metabolism, and B1 deficiency is thought to give rise to polyneuropathies. A group of male Wistar rats (n = 15) received a vitamin B1 deficient diet (group-a), and the pair fed control group (n = 20, group-b) received a normal diet with no vitamin deficiency. A second control group (group-c) was fed unrestrictedly with a standard diet (n = 19). All animals were examined for 25 weeks. The sensory nerve conduction velocity, the compound radicular, spinal and brain stem responses and the SEP were derived for tail and hind paw stimulation. The examination was repeated at 6-week intervals. There was no difference in nerve conduction between group-a and -b, but for both groups the conduction velocity was significantly slower than in group-c. The SEP latencies were significantly increased in group-a compared with group-b and also with group-c. The spinal and cerebral latencies were delayed in group-a. The diameters of myelinated nerve fibres were decreased in group-a compared with group-b, and in group-b compared with group-c. The results indicate that a specific polyneuropathy exists as a result of B1 deficiency, and that the sequelae of the lack of thiamine are pronounced in the CNS.

Afferent Pathways↗
PubMed1985

Ethanol and polyneuropathy.

Two groups of alcoholics (30 patients each)--identified by the MALT score--were examined. Clinical and laboratory investigations showed no connection between thiamine, riboflavin, or Vitamin B6 deficiency and development of the polyneuropathy. Neither the polyneuropathy nor the diminished sensory conduction velocity were related to malnutrition. The relation between the duration of alcoholism and symptoms of polyneuropathy was highly significant in one group. The neurotoxicity of ethanol was confirmed in an experiment with rats.

Adult↗
PubMedDate not supplied

Morphometry of the human cortex cerebri and corpus striatum during aging.

The commentary is based on our results of biological aging research with the following findings: a loss of neurons cannot be found, the size of neurons and the number of synapses decrease with age, the various regions of brain react quite differently. Altogether, these results speak for "use it or lose it." However, the genetically determined decreased brain function during aging can only be inhibited, not permanently preserved.

Aged↗
PubMedDate not supplied

[Morphometric studies of biological changes in synapses of the human caudate nucleus].

In the adult the caudate nucleus decreases with increasing age. Light microscopic measurements result in smaller sized neurons and a reduced neuropil in the aged. Comparable to a sintering, nerve cells move nearer, and in consequence, their density increases. The total number of nerve cells, however, does not change remarkably. Using electronmicroscopic methods the aging of synapses was morphometrically examined: Blocks of the caudate nucleus were cut out of formalin-fixed brains from 43 males who had died between the 22nd and 102nd year of age without any neurological symptoms. The blocks were contrasted with EPTA (ethanolic phosphotungstic acid) and embedded in araldite. Though the whole tissue was autolytic, presynaptic and postsynaptic densities were well-preserved and clearly visible. Their numbers and sizes were measured with semiautomatic procedures. The regression analysis shows a significant decrease of the density of synapses with increasing age. The aging of the human caudate nucleus is characterized by a decrease of its volume, by a reduction of the size of neuronal perikarya, and a diminution of the number of synapses per neuron. But the total number of nerve cells remains nearly constant.

Adult↗
PubMedDate not supplied

Establishing a nursing informatics program.

Nursing informatics is the synthesis of nursing science, information management science, and computer science to enhance the input, retrieval, manipulation, and/or distribution of nursing data. The literature abounds with articles stressing the unmet computer needs of nurses. Nurses must go beyond knowing computer terms and following basic program commands; they must be informatics competent. This article proposes five courses in nursing informatics to enhance all areas of nursing: practice, administration, education, and research.

Certification↗