Selective medium for MRSA.
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Bacteriological Techniques↗
Biomedical subjects
R Eggington
Publications and source records attributed to R Eggington.
Recovery of Mycoplasma hominis from blood culture media.
Although several workers have demonstrated that Mycoplasma hominis is responsible for 2 to 20% of cases of septicaemia, reports from Britain do not support this premise--possibly because many of the blood culture systems used fail to sustain growth of this organism. Previous work suggests that this is probably due to the inhibitory effects of sodium polyanethol sulphonate (Liquoid) on M. hominis. In an extension of an in-vitro evaluation of blood culture systems, gelatin was shown to give some protection against the inhibitory effect of polyanethol sulphonate on M. hominis. Of 20 systems evaluated, Becton Dickinson's Supplemented Peptone Broth in their Vacutainer collection system was the most successful at recovering M. hominis.
Blood↗
Evaluation of Malthus 128H microbiological growth analyser for detecting significant bacteriuria.
In a pilot study a prototype Malthus 128H microbiological growth analyser was used to study the growth of six commonly encountered urinary pathogens in a range of five different laboratory media. No single medium had been able to show acceptable conductance changes for all bacteria within five hours. Subsequently, the Malthus system, with fastidious anaerobe broth as the culture medium, was used to screen 500 consecutive clinical specimens of urine for significant bacteriuria. After two and a half hours the Malthus system detected 32 out of the 40 true positives and eight of the 44 contaminated urines. Increasing the detection time to five hours permitted the recognition of 39 true positives and 37 contaminated urines.
Bacteriological Techniques↗