[Cytotoxic effect of viscumin on hybridoma cells, synthesizing an antibody against the viscumin A-subunit].
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Animals↗
Biomedical subjects
R Eifler
Publications and source records attributed to R Eifler.
Differences in toxicity and antigenicity between mistletoe lectin I and viscotoxin A 3.
In contrast to mistletoe lectin I (ML I), viscotoxin A 3 does not inhibit protein synthesis in cell-free systems. From the immunological studies it is concluded that ML I and viscotoxin do not share identical structural domains.
Animals↗
[Incomplete spontaneous esophageal rupture - a variant of the Mallory-Weiss and Boerhaave syndrome?].
Belching and severe vomiting may lead in association with gastro-oesophageal prolapse to a variety of lesions within the gastro-oesophageal junction. Incarceration of the prolapse, diffuse hemorrhage from the prolapsed mucosa, longitudinal lacerations (Mallory-Weiss syndrome), intramural hematoma and spontaneous rupture (Boerhaave syndrome) are the most common complications. Based on 4 cases of incomplete rupture of the oesophagus we would like to draw attention to a disorder with mediastinal emphysema or pneumopericardium following severe vomiting. It is postulated that air escapes from a mucosal tear in the terminal oesophagus into the mediastinum. Conservative treatment seems justified.
Adolescent↗
The lectins from Agaricus edulis. Isolation and characterization.
2 lectins from the mushroom Agaricus edulis were isolated, after heating the crude extract at 75 degrees C, by ion exchange chromatography and gel chromatography using QAE-Sephadex A-50 and Sephadex G75. Some hemagglutinating and physicochemical properties of the agglutinins are reported.
Agaricales↗
Chemical modification studies on the D-galactopyranosyl binding lectin from the mistletoe Viscum album L.
The role of amino, sulfhydryl, disulfide, carboxyl, phenolic, imidazole and indole groups on the agglutination of human erythrocytes by the lectin from Viscum album has been determined using specific chemical modification techniques. The results indicate that tyrosine residues participate in the hemagglutination reaction. Subunits of the lectin possess only reduced hemagglutinating ability.
Borohydrides↗
On the origin of "isozymes" of inorganic pyrophosphatase obtained from baker's yeast.
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Electrophoresis, Disc↗
The subunit structure of inorganic pyrophosphatase from baker's yeast.
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Chromatography, DEAE-Cellulose↗
[Study of plant lectins from Viscum album using monoclonal antibodies].
Monoclonal antibodies (monAT) against both native (TA5, TB12) and denatured (TB33, TB35) plant toxin ML1 from Viscum album have been obtained. The interaction of monAT against native toxin with its isoforms ML2 and ML3 was investigated. It was shown that monAT TA5 to A-chain of ML1 toxin cross-reacted with ML2 and ML3 isoforms. TA5 did not inhibit enzyme activity of A-chain in cell-free rabbit reticulocyte system. It was shown that monAT TB12 reacted with galactose-binding site of B-subunit. Both monAT had no cross-reactions with plant toxin ricin. The binding constants for TA5 with ML1, ML2, ML3 respectively were 4.3.10(7) M-1, 1.2.10(7) M-1, and 0.3.10(7) M-1. The binding constants for TB12 were 2.10(7) M-1 with ML1 toxin, and more than 10(6) M-1 with ML2 and ML3. The nature of heterogeneity in ML toxin family is discussed. Test-systems for ML1 determination in different V. album extracts are suggested.
Animals↗